Sterol-Response Pathways Mediate Alkaline Survival in Diverse Fungi

The work described here further elucidates how microorganisms sense and adapt to changes in their environment to establish infections in the human host. Specifically, we uncover a novel mechanism by which an opportunistic human fungal pathogen, Cryptococcus neoformans, responds to increases in extracellular pH in order to survive and thrive within the relatively alkaline environment of the human lung. This mechanism, which is intimately linked with fungal membrane sterol homeostasis, is independent of the previously well-studied alkaline response Rim pathway. Furthermore, this ergosterol-dependent alkaline pH response is present in Candida albicans, indicating that this mechanism spans diverse fungal species. These results are also relevant for novel antimicrobial drug development as we show that currently used ergosterol-targeting antifungals are more active in alkaline environments.

exposed to wide fluctuations in pH as they move between various microenvironments in the human host. Among these, fungi that cause invasive fungal infections (IFIs) have acquired the ability to rapidly adapt to changes in extracellular pH to promote their survival during an infection. The shift of a fungal pathogen from an acidic external environment to the neutral/alkaline pH of the mammalian host is associated with the activation of the fungus-specific Rim/Pal signaling pathway, triggering cellular changes important for survival under these new conditions. These changes include alterations in the cell wall, often accompanied by larger morphological transitions that promote host colonization. In the common fungal pathogen Candida albicans, pH-directed cellular responses include the ability to transition between yeast-like growth and invasive hyphal forms (1)(2)(3). The opportunistic human fungal pathogen and basidiomycete yeast Cryptococcus neoformans similarly activates Rim signaling to respond to changes in pH. Because C. neoformans initially colonizes the human lung, which is often relatively more alkaline than its natural environmental reservoirs, this signaling pathway is activated in the setting of infection. In fact, the C. neoformans Rim101 transcription factor, the terminal component of the Rim pathway, is among the most highly induced transcripts in vivo (4).
Given its pH-dependent activation, as well as its important role in the adaptation of fungal cells to elevated pH, the Rim signaling cascade is often considered to be the major alkaline pH response pathway in fungi. However, other cellular processes and pathways are required for fungal growth under conditions of extreme pH (both acidic and alkaline). These processes include the production of glycosphingolipids (GSLs) that associate with proteins in the outer leaflet of fungal plasma membranes to form lipid rafts and maintain membrane fluidity and organization (5)(6)(7). Recent studies have demonstrated that mutations resulting in reduced or absent GSLs render fungi such as Kluyveromyces lactis, Neurospora crassa, and C. neoformans unable to grow in alkaline environments (8)(9)(10)(11). The connection between membrane composition and the ability for fungal cells to grow in alkaline environments has been associated with defects in cytokinesis and altered activity of plasma membrane proton pumps, as well as an altered lipid profile (10). Furthermore, reduced ergosterol content in membranes has been linked to salt stress sensitivity in Saccharomyces cerevisiae (12,13) and to aberrant V-ATPase regulation of pH gradients in Candida albicans (13,14).
Recent observations from our genetic screen suggest that C. neoformans sterol homeostasis might also be required for growth at elevated pH (15). The sterol homeostasis pathway (SREBP pathway) has been extensively studied in both mammalian and fungal cells (16)(17)(18)(19)(20). Proteins in this pathway regulate the production and delivery of sterols to the plasma membrane to maintain appropriate cell homeostasis (17,21,22). In several fungal species, including C. neoformans, the Sre1 transcription factor (the terminal transcription factor in this sterol homeostasis pathway) is activated in response to low-oxygen conditions (21,(23)(24)(25)(26). In addition to hypoxia, the C. neoformans Sre1 transcriptional response is necessary for tolerance to low iron and to antifungals that target sterols in the membrane (21). Upon activation of the C. neoformans sterol homeostasis pathway, the basidiomycete-specific Stp1 protease cleaves Sre1, freeing its N terminus to release from the membrane of the endoplasmic reticulum and translocate to the nucleus (22). This cleavage is induced in an O 2 -dependent manner and is important for the transcription of many ergosterol biosynthesis genes (23,25). However, the association between the sterol homeostasis pathway and pH adaptation has not yet been explored.
Here, we define potential interactions among fungal sterol homeostasis, alkaline pH tolerance, and Rim pathway activation. We find that the sterol homeostasis pathway is indeed necessary for growth in an alkaline environment and that an elevated pH is sufficient to induce Sre1 cleavage and activation. This pH-mediated activation of the Sre1 transcription factor is not dependent on Rim pathway signaling, suggesting that these two pathways are responding to alkaline pH independently. Furthermore, we demonstrate that Sre1-mediated ergosterol biosynthesis is linked to the response to alkaline pH and relevant in biologically diverse fungi. Finally, we discover that C.
neoformans is more susceptible to membrane-targeting antifungals under alkaline conditions, highlighting the impact of microenvironmental pH on the treatment of this invasive fungal infection. Together, these findings connect a highly conserved pathway involved in membrane homeostasis and sterol maintenance to the adaptive response to changes in extracellular pH. extract-peptone-dextrose [YPD] medium). These two mutants also displayed similar growth defects as the wild type on growth medium buffered to a pH greater than 7 (Fig. 1A). Importantly, the sre1Δ alkaline pH-sensitive mutant phenotype was rescued by the reintroduction of the wild-type SRE1 allele (see Fig. S1A in the supplemental material).
To account for a potential confounding effect on growth by exogenous lipids in the yeast extract-rich medium, we also assessed the ability for these mutant strains to grow on a minimal medium (yeast nitrogen base [YNB]) buffered to a range of pH values. The sre1Δ and rim101Δ mutants were able to grow on YNB medium buffered to pH 4 through pH 7. At more alkaline pH, the growth defect of the sre1⌬ mutant strain was more severe than that of the rim101Δ mutant, with the sre1Δ mutant unable to grow at pH 8 and the rim101Δ strain displaying complete growth inhibition only at pH Ͼ8 (Fig. 1B).
Given the established role of Sre1 in mediating growth in hypoxia, we compared growth rates of these mutant strains in the presence of cobalt chloride (CoCl 2 ), an agent that disrupts many biochemical pathways, including the ergosterol biosynthesis pathway and cellular respiration (27)(28)(29)(30). Consistent with previous reports (21,24,25,31), the sre1Δ mutant is unable to grow under these conditions ( Fig. 1D and Fig. S1A). The rim101Δ mutant did not share this growth defect and was not sensitive to CoCl 2 (Fig. 1D). We also compared growth rates of all mutant strains in a microaerophilic chamber to more directly test phenotypes in response to reduced oxygen. The sterol homeostasis pathway mutants displayed a lower growth rate under conditions of reduced oxygen concentration (Fig. S1B). The rim101Δ mutant grew to similar levels as the wild type (Fig. S1B). Therefore, although sharing a similar alkaline growth defect, the rim101Δ and sre1Δ mutants display distinct growth patterns under hypoxia-like conditions.
We also tested the sensitivity of the sre1Δ and rim101Δ mutant strains to cell wall stressors such as Congo red (interferes with beta glucan-chitin linkages), caffeine (affects cell wall integrity), high salt (osmotically stresses the cell wall), and SDS (stresses the cell membrane) (32,33). Similarly to alkaline pH, high salt resulted in complete growth inhibition for both mutant strains (Fig. 1C). In contrast, caffeine did not affect the growth of either mutant (Fig. 1C). The sre1Δ mutant strain was unable to grow in the presence of Congo red, whereas the rim101Δ mutant strain showed only a subtle growth defect due to this chitin polymer inhibitor (Fig. 1C). Also, SDS completely inhibited growth of the sre1Δ strain, whereas the rim101Δ strain appeared to be hyperresistant to the membrane-targeting effects of SDS, as evident in the more robust growth of this strain than of the wild type (32,34) (Fig. 1C).
Sensitivities of mutant strains to cell surface stressors can indicate alterations in the cell wall structure and/or integrity. In addition to providing a protective barrier for the cell, the cell wall serves as an anchor for the attachment of the polysaccharide capsule that can further protect the fungal cells during a human infection (35). The rim101Δ mutant strain is known to have a disorganized cell wall and thus a decrease in attached capsule (36,37). In contrast, the sre1Δ mutant strain revealed intact capsule formation (38) (Fig. 1E). Overall, these phenotypic comparisons distinguish the rim101Δ mutant from the sre1Δ mutant in the distinct responses of these strains to cell wall and membrane stress.
Independent signaling of the Rim and sterol homeostasis pathways. To determine whether the C. neoformans sterol homeostasis pathway is specifically activated in response to alkaline pH, we assessed the pH dependence of the proteolytic cleavage of the Sre1 transcription factor, a marker of pathway activation (21,(23)(24)(25)38). At pH 5.5, the green fluorescent protein (GFP)-Sre1 fusion protein remains uncleaved in a 140-kDa form ( Fig. 2A). In contrast, incubating this strain in the same growth medium buffered to pH 8 results in GFP-Sre1 protein cleavage to a 90-kDa form, similar to its proteolytic activation in response to hypoxia ( Fig. 2A) (21). There was no defect in Sre1 cleavage in the rim101⌬ mutant strain background (Fig. 2B). Therefore, the C. neoformans sterol homeostasis pathway is specifically activated by an alkaline pH signal and in a manner that is independent of the Rim alkaline response pathway.
To further define the interaction between the Sre1 and Rim101 signaling pathways, we assessed whether the Sre1 transcription factor is necessary for activation of the Rim pathway as measured by the pH-dependent proteolytic processing and subcellular localization of the Rim101 transcription factor (39). In both the wild-type and sre1Δ mutant strains, we observed intact Rim101 processing and cleavage at elevated pH (Fig. 2C). Similarly, GFP-Rim101 nuclear localization was enhanced at activating pH in both strain backgrounds (Fig. 2D). In contrast, we confirmed both defective protein cleavage and impaired nuclear localization of the Rim101 transcription factor in the rim20Δ mutant, a strain lacking a known upstream Rim signaling component ( Fig. 2C and D). These data indicate that the sterol homeostasis pathway is not required for Rim pathway activation.
The cell wall organization of the sre1⌬ mutant and its in vitro immune phenotypes. The sre1Δ mutant strain is avirulent in a mouse model of C. neoformans infection (25,38), whereas the rim101Δ mutant strain and other Rim pathway mutants have paradoxical hypervirulent phenotypes in the same model (36). In previous work, processing under low-pH and high-pH growth conditions. The GFP-Rim101 fusion protein is cleaved from its 140-kDa form to its active 100-kDa form at pH 8. Similarly, the GFP-Sre1 fusion protein is proteolytically processed from 146 kDa to approximately 90 kDa in response to alkaline pH. Indicated strains were incubated for 60 min in either pH 5.5 or pH 8 YPD medium prior to lysing. Rim101 and Sre1 protein processing was determined using a GFP-trap pulldown and Western blotting using an anti-GFP antibody. Protein levels were normalized prior to loading. (B) Western blot analysis of the Sre1 protein in different genetic backgrounds revealed the cleavage and processing (from 110 kDa to approximately 90 kDa) of the Sre1 transcription factor in the WT and rim101Δ mutant backgrounds. Indicated strains were incubated for 60 min in either pH 4 or pH 8 YPD medium prior to lysing. Protein processing was determined through protein A pulldown and Western blotting using a polyclonal anti-Sre1 antibody. Total protein levels are represented by a PSTAIR loading control. (C) The Sre1 protein is cleaved in response to alkaline pH. The eGFP-RIM101 allele was expressed in the WT, rim20Δ mutant, and two independent sre1Δ mutant strains (sre1Δ #1 and sre1Δ #2). The untagged WT strain and the eGFP-Rim101-expressing strains were incubated in YPD medium pH 4 or pH 8 for 60 min. Rim101 processing was assessed using a GFP-trap pulldown and Western blotting using an anti-GFP antibody. Protein levels were normalized prior to loading. (D) The indicated strains (the same as in panel C) were incubated in synthetic complete medium buffered to pH 4 or pH 8 for 60 min. Rim101 localization was assessed by epifluorescence microscopy, and alkaline-induced nuclear localization was compared to the eGFP-Rim101 positive control. White scale bars indicate 5 m.
Sterols and the Fungal Response to Alkaline Stress ® we demonstrated by transmission electron microscopy that the rim101Δ mutant has an aberrant, thick, and disorganized cell wall in comparison to wild-type cells (36). We probed the rim101Δ and sre1Δ mutant strains with calcofluor white (CFW) and wheat germ agglutinin (WGA) to assess total and exposed levels of chitin, respectively. In both mutant strains, we noted similar increases in cell wall chitin levels as measured by CFW staining. However, the level of exposed chitin (WGA) was increased only in the rim101⌬ strain. The intensity of the WGA fluorescence was quantified by measuring brightness intensity (Fiji) in photomicrographs (Fig. 3A) as well as by flow cytometry (Fig. S2). The observed increase in total chitin levels can be a nonspecific response to cell stress (40). However, increased chitin exposure, as assessed by intensity of WGA staining, has been previously demonstrated to correlate with the degree of macrophage activation in vitro (36,41). Together, these cell wall analyses suggest that the Rim pathway and sterol homeostasis pathway induce distinct microbial physiological responses to host-like conditions. Specifically, the Sre1-mediated response to host stress does not include increased exposure of chitin.
To further define the extent to which these cell wall epitopes may affect virulence, we assessed macrophage interactions with the sre1Δ mutant compared to the rim101Δ mutant strain. Macrophages are among the first immune cells encountered by this pathogen when infecting its host in the human lung. We therefore quantified fungal survival after coculturing stimulated J774A.1 murine macrophage-like cells with the wild-type, rim101Δ, and sre1Δ strains. Following coincubation with macrophages, the rim101Δ mutant strain displayed increased survival compared to wild type, as has been shown previously (37) (Fig. 3B). The sre1Δ mutant strain displayed a moderate, reproducible reduction in viability in the presence of macrophages compared to the wildtype strain. This result was consistent with the previously reported attenuated virulence of the sre1Δ mutant strain in animal models of infection (25,38). The significantly different patterns of macrophage interaction of the sre1Δ and rim101Δ mutant strains further suggest that distinct downstream cellular processes are controlled by these alkaline-responsive pathways (Fig. 3B).
Ergosterol biosynthesis is required for growth at alkaline pH in C. neoformans and other fungal pathogens. Our data support that the Rim and sterol homeostasis pathways are independent cell signaling pathways that each mediate adaptive responses to alkaline stress. Given the established role of fungal Sre1 orthologs in the regulation of membrane sterol content, we hypothesized that alterations in minor membrane lipids, especially ergosterol, might be involved in the adaptive response to alkaline pH. Previous work in C. neoformans sterol homeostasis documented decreased ergosterol levels in the sre1Δ mutant strain (22,25). The sre1Δ alkaline pH sensitivity was rescued by the addition of exogenous ergosterol to the growth medium in a dose-dependent manner (Fig. 4A). Importantly, addition of exogenous sterols did not affect the pH of the growth medium. This observation is similar to prior investigations showing growth rescue of various S. cerevisiae ergosterol biosynthesis mutants by supplementation with exogenous ergosterol (42). These data suggest that intact ergosterol induction and homeostasis are specifically required for fungal adaptation to alkaline pH.
To further explore the role of ergosterol biosynthesis in the alkaline pH response, we tested three C. neoformans ergosterol-related mutants for growth at pH 8, and all shared an alkaline pH growth defect (Fig. 4B). Many steps in ergosterol biosynthesis are essential for growth under routine conditions, limiting the availability of ERG gene mutants. The nonessential ERG4 and ERG6 genes encode terminal enzymes in the ergosterol biosynthesis pathway (22,43). Compared to wild type, the erg4Δ and erg6Δ mutants displayed a specific growth defect at alkaline pH ( Fig. 4B and C) (15). Similarly, the CNAG_00490 locus encodes a putative acetyl coenzyme A (acetyl-CoA) acetyltransferase, as does the ERG10 (CNAG_02918) gene. The loss-of-function cnag_00490⌬ mutant also displays alkaline pH sensitivity (Fig. 4B). The pH sensitivity of the CNAG_00490 mutant as well as the predictive function of its gene product suggests that and wheat germ agglutinin (WGA). Cells were incubated in CO 2 -independent medium for 18 h at 37°C. Cells were stained with FITC-conjugated WGA and CFW and incubated in the dark for 35 min and 10 min, respectively. Mean fluorescence intensity was quantified for each strain and each condition. Two-way ANOVA and Tukey's multiple-comparison test were run to determine statistical significance. White scale bars indicate 5 m. ****, P value Ͻ 0.0001. DIC, differential inference contrast. (B) When grown in the presence of J774A.1 macrophages, the rim101Δ mutant strain can survive significantly better than both the wild-type and the sre1Δ mutant strain. Indicated strains were coincubated with macrophages for 24 h, and survival was determined by quantitative cultures. One-way ANOVA and Tukey's multiplecomparison tests were run to assess statistical significance between fungal cell survival percentages. Six biological replicates of each strain were analyzed. **, P value Ͻ 0.003; ***, P value Ͻ 0.0002. it might participate in the conversion of acetyl-CoA to squalene, an early step in sterol synthesis.
Ergosterol is a major component of most fungal membranes, including those of distantly related fungal pathogens in the ascomycete phylum. To further explore the The reduced growth rate of the sre1Δ mutant strain in liquid growth medium at pH 8 can be rescued through the addition of exogenous ergosterol in a dose-dependent manner. Growth rate of indicated strains was assessed by changes in OD 595 in biological triplicate every 10 min for 42 h at 30°C. Ergosterol was added as indicated. One-way ANOVA and Dunnett's multiple-comparison test were run on the last time point under each condition compared to the pH 8-alone condition to determine statistical significance. **, P value Ͻ 0.003; ***, P value Ͻ 0.0005; ns, not significant. (B) Other sterol-related mutants exhibit alkaline pH sensitivity. Two deletion mutants related to ergosterol biosynthesis in C. neoformans (erg4⌬ and cnag_00490Δ) display a pH sensitivity when grown on pH 8 growth medium. Indicated strains were serially diluted onto YPD medium and YPD-150 mM HEPES pH 8. Growth was compared to WT and assessed after 3 days. (C) erg6Δ C. neoformans mutant also exhibits alkaline pH sensitivity when grown on pH 8 medium. Indicated strains were serially diluted onto YPD medium and YPD-150 mM HEPES pH 8. Growth was compared to WT and reconstituted strains and assessed after 3 days. (D) Candida species ergosterol mutants reveal similar pH-sensitive phenotypes. C. albicans and C. lusitaniae wild-type strains and strains with mutations in various components of ergosterol biosynthesis were serially diluted onto YPD medium and YPD-150 mM HEPES pH 8 and 8.5 as well as YES medium with 7 mM CoCl 2 . Growth was compared to WT and assessed after 2 days. association between sterol homeostasis and alkaline pH response, we tested alkaline pH survival for ergosterol biosynthesis mutants in two Candida species, C. albicans and C. lusitaniae (Fig. 4D). The homozygous diploid C. albicans erg6/erg6 and erg24/erg24 mutants displayed severe growth defects at high pH that were not evident under more acidic conditions (Fig. 4D). Similarly, the haploid C. lusitaniae erg6 mutant had impaired growth compared to wild type under alkaline conditions (Fig. 4D). These results suggest a conserved requirement for efficient sterol maintenance in the adaptation to alkaline pH among highly divergent fungal species.
Sre1 regulates membrane-associated transcripts under alkaline growth conditions. The C. neoformans SRE1-dependent transcriptome has been defined in the context of the cellular response to low oxygen (21,22,38). These prior studies revealed that Sre1 is required for the induction of genes involved in ergosterol homeostasis in an oxygen-dependent manner. However, given the novel role for Sre1 pathway activation at alkaline pH, we defined the pH-responsive Sre1-regulated transcriptional response. Comparison of the transcriptomes of the sre1Δ mutant and wild type after 1.5 h of growth in alkaline pH revealed 2,655 transcripts that were differentially regulated in a statistically significant manner (adjusted P value of Ͻ0.05) ( Fig. S3A and Table S1). This represents approximately one-quarter of the C. neoformans genome, indicating that Sre1 has a major impact on the cell in response to pH stress. Similar to the transcriptome studies in hypoxia, transcript abundance of the majority of the ERG genes (13/18) and the STP1 activating protease was differentially regulated at alkaline pH ( Fig. 5A and B). The stp1Δ mutant strain displays a pH-sensitive mutant phenotype similar to the sre1Δ mutant strain (15). Importantly, ERG3 transcript levels had the highest relative fold change in the sre1Δ mutant at high pH compared to wild type ( Fig. 5A and B). ERG3 encodes a component of the ergosterol biosynthesis pathway and displays similar Sre1-dependent expression under low-oxygen conditions (22).
Due to the large number of differentially expressed transcripts identified in this analysis, we performed a modified Gene Ontology (GO)-term analysis using FungiDB on genes with a 2-fold or greater change in transcript abundance in the sre1Δ mutant compared to wild type (44). Genes repressed in the sre1Δ mutant at high pH are enriched for biological processes such as aldehyde synthesis, cellular respiration/ oxidoreduction, membrane composition, phosphorylation regulation, and transmembrane transport. Genes that are induced in this mutant background under alkaline conditions are involved in cellular respiration/oxidoreduction, membrane composition, sulfur metabolism, and transmembrane transport ( Fig. 5C and Table S1). Interestingly, although some of these GO terms are shared with the previously published SRE1 transcriptome under 3% oxygen conditions, the majority of the Sre1-dependent transcripts differ between the two experimental inducing conditions: hypoxia versus alkaline pH (22) (Fig. S4). Using the same fold change values to compare these transcript data sets, only nine genes are induced under both conditions, the majority of which are related to ergosterol biosynthesis: SRE1, ERG3, ERG11, ERG6, ERG4, and ERG13 (Fig. S4). This transcriptome analysis supports the central role for ergosterol biosynthesis genes as potential Sre1-dependent effectors of both hypoxia and the response to alkaline pH. We also documented that different inducing conditions mediate distinct Sre1dependent transcriptional responses.
We were also able to define groups of genes in the wild-type strain that are either induced or repressed following the shift from low to high pH. These groups include a significant portion of membrane-associated transcripts, including integral membrane components, composition regulators, and membrane transporters ( Fig. S3C and D and Table S1). Transcripts with increased abundance in response to alkaline pH include many of the known Rim pathway regulators (RIM101 and RIM23) and pathway outputs (ENA1, CIG1, and SKN1) ( Fig. S3C and Table S1). Consistent with prior reports of the involvement of Sre1 in iron homeostasis (21,45), we also identified an iron transporter (CNAG_00815), suggesting a conserved role for iron regulation to adapt to changes in extracellular pH (Table S1). Furthermore, many genes involved in membrane composition, glucose/complex carbohydrate metabolism, and regulation of protein phosphor-ylation were induced under alkaline conditions (Fig. S3C). Complex carbohydrates are major components of the fungal cell wall, supporting previous findings that the Rim-mediated pH response is linked to the reorganization of the cell wall (36). GO-term analysis of transcripts with reduced abundance at high pH revealed genes involved in membrane transport, potentially in an effort to regulate import of extracellular ions into the cell (Fig. S3D). This analysis revealed no clear repression of membrane composition transcripts at high pH ( Fig. S3D and Table S1).
pH affects efficacy of membrane-targeting antifungals. Given our observation of a correlation between fungal sterols and growth at alkaline pH, we tested the pHdependent efficacy of antifungal agents targeting different aspects of membrane ergosterol homeostasis. Amphotericin B (AMB) is a polyene antifungal that removes ergosterol from fungal membranes (46). We observed a dramatic reduction in the AMB MIC for wild-type C. neoformans cells grown on YPD pH 8 (0.25 g/ml) compared to YPD pH 5.5 (2 g/ml) (Fig. 6A). Furthermore, the time-dependent killing of fungal cells by AMB increased in a pH-dependent manner, further supporting that this drug has a higher efficacy under alkaline growth conditions (Fig. 6B). We also found that AMB was significantly more efficacious against the sre1Δ strain (MIC ϭ 0.00125 M) than the wild FIG 5 Transcriptomic analysis of the sre1Δ and wild-type strains in response to alkaline pH. WT and sre1Δ cells were incubated in YPD medium pH 4 or pH 8 for 90 min. This experiment was conducted with six biological replicates for each strain and condition. Total RNA was extracted, mRNA was isolated, and libraries were prepared and finally sequenced using an Illumina NextSeq 500 sequencer. GO-term analysis was performed using FungiDB. (A) The majority of the known genes in C. neoformans ergosterol biosynthesis were significantly differentially expressed in the sre1Δ versus wild-type transcriptome at pH 8. ERG genes that were significantly differentially expressed have an adjusted P value of Ͻ0.016 (teal, repressed in the sre1Δ mutant compared to wild type; gray, induced in the sre1Δ mutant compared to wild type). (B) Volcano plot displaying the significantly regulated transcripts in the sre1Δ versus wild-type transcriptome at pH 8 (adjusted P value of Ͻ0.05) (teal, repressed in the sre1Δ mutant compared to wild type; gray, induced in the sre1Δ mutant compared to wild type). The full volcano plot (zoomed out) is shown in Fig. S2. (C) GO-term analysis of the sre1Δ versus wild-type differentially expressed genes following a 90-min shift from YPD pH 4 to YPD pH 8. These transcripts were selected based on a strict cutoff of log 2 fold change of Ϯ1. Teal, biological processes repressed in sre1Δ mutant compared to wild type at high pH; gray, biological processes induced in sre1Δ mutant compared to wild type. type when the cells were grown at low pH (pH 4 to 6) (Fig. 6B). The significant increase in AMB activity against this mutant strain with reduced ergosterol content is consistent with our model that disruption in fungal sterols leads to pH sensitivity. Furthermore, in a drug disc diffusion assay using pyrifenox, a drug used to treat phytopathogens through inhibition of ergosterol biosynthesis (47), there was a significantly greater zone of clearance and inhibition of growth of wild-type C. neoformans cells when grown on medium buffered to pH 8 compared to pH 5.5 (Fig. 6A).
Fluconazole is an antifungal that inhibits the activity of Erg11, an important component of the ergosterol biosynthesis pathway. We hypothesized that removing ergosterol from the cell membrane in this way would cause a similar sensitivity to alkaline pH as we observed with the ergosterol mutant strains in various fungal pathogens (Fig. 4). In contrast to the major pH-dependent activity of AMB and pyrifenox, we observed a reproducible but more subtle effect of pH on fluconazole efficacy. The fluconazole MIC was 2-fold lower for wild type at pH 8 (16 M) compared to YPD pH 5.5 (32 M) (Fig. 6A). The azoles and polyenes have been shown in other organisms, such as Aspergillus fumigatus, to have variable activity against invasive fungal infections depending on the pH of the growth environment (48). Similar to the findings in A. fumigatus, these data support that increases in alkalinity allow for higher efficacy of specific polyenes and azoles against C. neoformans. Our data reveal that reduction of ergosterol, either genetically or biochemically using known antifungals, leads to reduced growth in alkaline environments. Altogether, these results further inform the Sterols and the Fungal Response to Alkaline Stress ® connection between fungal plasma membrane homeostasis, the molecular interactions that drive environment sensing, and the ability for a biologically diverse group of fungi to grow in increasingly alkaline environments, including their human host.

DISCUSSION
Novel, Rim-independent pH-sensing pathway in C. neoformans. These experiments support a model in which several cell processes and signaling pathways work together to allow microbial growth under stress conditions such as elevated pH. The Rim signaling pathway has been identified in multiple fungal species including C. neoformans, C. albicans, and S. cerevisiae as a major signaling response to increases in extracellular pH (1-3, 15, 36, 37, 39, 49-53) (Fig. 7). Its primary function appears to be translating extracellular alkaline pH signals to control adaptive changes in the fungal cell wall (36) (Fig. 7B). Data presented in this study identified the sterol homeostasis pathway as a unique mechanism that responds to alkaline pH in a Rim-independent way (Fig. 7).
The sterol homeostasis pathway has been implicated in the response to alterations in oxygen availability, membrane ergosterol levels, and various stressors in diverse fungal species. In the fission yeast, Schizosaccharomyces pombe, the induction of ergosterol biosynthesis genes by the Sre1 transcription factor and its chaperone proteins (Scp1 and Ins1) has been well characterized in response to hypoxia (18,20,54). C. neoformans, similarly to S. pombe, has a well characterized Sre1-mediated response to hypoxia that results in the induction of ergosterol biosynthesis genes to maintain membrane homeostasis (21,25,27,34,38). However, in C. neoformans, a basidiomycete-specific protease has been identified that specifically activates Sre1 in response to hypoxia (Fig. 7A) (22, 38). Elements of this pathway have also been The activating sensor for the sterol homeostasis pathway is unknown and could be linked to alkaline pH-induced reductions in ergosterol/ membrane alterations or bioavailable iron. In response to alkaline pH, the Sre1 transcription factor is cleaved, activated, and localized to the nucleus to aid in the transcription of many genes involved in ergosterol biosynthesis and membrane homeostasis. This cleavage and activation are dependent on both the conserved transmembrane protein Scp1 and the basidiomycete-specific protease Stp1. (B) The Rim alkaline response pathway is signaled through the transmembrane pH sensor Rra1 and its interaction with the plasma membrane. At elevated pH levels, Rra1 is endocytosed, allowing it to interact with the downstream components of the pathway and propagate the signal to the endosomal membrane complex (ESCRT components, Rim23, and Rim20) and activate the Rim13 protease. This protease cleaves the Rim101 transcription factor, allowing it to translocate to the nucleus and induce the expression of genes involved in cell wall and surface remodeling. identified in the filamentous fungal pathogen A. fumigatus. The Sre1 homolog, SrbA, is essential for the ability of this pathogen to grow in environments with limited oxygen or low iron or in the presence of membrane-targeting antifungals (19,23,45,55,56). This hypoxic response is required for survival in the infected host in which hypoxic microenvironments exist, especially in poorly viable tissue such as necrotic tumors and wounds (57). The dimorphic fungal pathogen Histoplasma capsulatum also contains a homolog of Sre1 (Srb1) that is essential for the response to hypoxia as well as for virulence (58)(59)(60). Other yeasts such as C. albicans and S. cerevisiae do not contain genes in their sequenced genomes encoding obvious SREBP homologs. Instead, these species respond to hypoxic stress through the activation of a different transcription factor, Upc2, which directs the induction of ergosterol biosynthesis genes (61,62). However, the C. albicans Cph2 protein binds SRE1-like elements in the genome, and it may therefore be a functional ortholog of Sre1 (63).
The identification of a new role for the sterol homeostasis pathway is informative to better conceptualize and target fungal pathogenesis in general and cryptococcal pathogenesis in particular for several reasons. First, the sterol pathway in C. neoformans has a basidiomycete-specific Stp1 protease that is required for cleavage and activation of Sre1 (22,25,38). Genes encoding a similar protease are found in the genomes of other basidiomycete fungi such as Cryptococcus gattii, Malassezia globosa, and Mucor circinelloides (44) and not in those of more distantly related fungi or higher eukaryotes. This fungal specificity and distinction from the mammalian sterol homeostasis pathway (16,17,64) may provide an interesting future target for novel antifungals. Second, understanding the extracellular cues that activate this pathway may elucidate more detailed signaling mechanisms controlling sterol homeostasis, potentially revealing some currently unknown upstream components. Presently, it is not known if a common signal in hypoxia or alkaline pH initiates Sre1 signaling, or if multiple upstream Sre1 activators are present (Fig. 7A). The C. neoformans sterol homeostasis pathway is lacking an obvious INSIG homolog as well as a site-1 protease (24). Elucidating the Sre1mediated response to alkaline pH through further analysis of our forward genetic screen may uncover either functional orthologs of these proteins or novel pathway components that mediate specific stress responses in C. neoformans.
The transcriptional analysis of the sre1Δ mutant strain at high pH provided further support for the distinct activation of the Sre1 transcription factor in response to increases in extracellular pH. This type of analysis has been conducted for the C. neoformans sre1Δ mutant strain previously but with conditions of low and high oxygen availability (22). When comparing our transcriptomics data to this previously published microarray analysis, the majority of the transcripts were nonoverlapping, suggesting independent downstream effectors of Sre1 in response to specific stress (see Fig. S4 in the supplemental material). Furthermore, there was no overlap between the Sre1associated transcriptome at high pH and the previously published Rim101-associated transcriptome at a similar pH, further supporting the distinct nature of these two pH response mechanisms and the specificity of the Sre1-mediated response to alkaline pH stress (data not shown and Fig. 7

) (15).
Ergosterol biosynthesis is essential for the ability of fungal pathogens to grow in an alkaline environment. The generation of ergosterol for overall fungal membrane integrity has been well studied in the response to extracellular stresses such as hypoxia and low iron (23,24,26,38,45,55,56). Ergosterol controls the fluidity and structure of fungal cells (65), and it is needed for the formation of microdomains within the membrane containing ion pumps and transmembrane proteins necessary for cellular growth and signaling (12,13,(65)(66)(67). In this study, we have demonstrated that supplementing pH-sensitive mutant strains with ergosterol can rescue the pH-sensitive mutant phenotype, suggesting that the sre1Δ mutant pH sensitivity is specifically linked to its ergosterol deficiency.
Our studies further supported this link between ergosterol and the pH response through analysis of the effects of alkaline pH on the biosynthesis of ergosterol at the transcriptional level. In response to a shift in pH, the majority of the known C. neoformans ergosterol biosynthesis genes were differentially regulated in the sre1Δ strain compared to wild type. These results support our model and implicate Sre1mediated membrane homeostasis as a direct response to alkaline stress (Fig. 7A). Furthermore, C. neoformans and C. albicans strains with mutations in known and predicted ergosterol synthetic processes were unable to grow at alkaline pH. These results indicate that ergosterol levels and membrane homeostasis are important in the pH response mechanisms of many fungal species. This broadens these findings from Sre1-specific regulation of ergosterol affecting pH growth of a basidiomycete fungal pathogen to general ergosterol maintenance affecting the pH response in many different fungal pathogens across phyla.
In addition to establishing a link between alkaline pH and membrane sterols, our data also support emerging data on the interplay between the pH of the external environment and iron homeostasis (Fig. 7A). In divergent cell types, bioavailable iron concentrations are often reduced at alkaline pH (68). Our data demonstrate the induction of an iron transporter transcript in response to alkaline pH (Table S1), further suggesting that the cell is responding to reduced iron availability under this condition. In A. fumigatus, supplementing ⌬srbA mutants with exogenous iron rescues growth defects in low oxygen and during azole treatment (45). Also, in the dimorphic fungal pathogen H. capsulatum, Sre1 signaling mediates the ability of this fungus to survive under hypoxic conditions as well as to control iron regulation. Each of these processes may mediate separate roles in fungal virulence (59, 60) Furthermore, prior investigations have also demonstrated that the C. neoformans Sre1-mediated stress response is linked to iron availability (21). Future studies will determine if exogenous iron will fully or partially suppress the C. neoformans sre1Δ mutant pH growth defects in a similar manner as exogenous sterols.
Ergosterol-depleting antifungals render cryptococcal cells sensitive to alkaline pH. Our results have shown not only that genetic manipulation of fungal membrane homeostasis and ergosterol biosynthesis can increase the sensitivity of C. neoformans to alkaline pH but also that biochemical and pharmaceutical interventions have the same effect. We tested relevant antifungals that prevent sterol production or directly deplete sterols from fungal membranes and demonstrated that the activity of these drugs improves in neutral/alkaline environments. AMB, an antifungal that directly disrupts the plasma membrane through sequestration of ergosterol (46), was significantly more potent with increases in the pH of the growth environment. Similarly, fluconazole and pyrifenox, drugs that inhibit the ergosterol biosynthesis pathway (47,69), were also more effective at alkaline pH. These results reflect similar findings in Aspergillus species treated with itraconazole and AMB (48). Similar studies using ketoconazole, AMB, and flucytosine (5-FC) against Candida species showed that the in vitro drug activity increases as a function of pH (70,71). Interestingly, there has also been one study demonstrating increased efficacy of 5-FC against C. neoformans at higher pH (72). The fact that flucytosine does not directly target the cell membrane, together with the subtle alterations in fluconazole activity as a function of pH, suggests that multiple factors control this phenomenon. However, our findings that known ergosteroltargeting antifungals render diverse fungi more vulnerable to growth environments with increasing pH further support our leading hypothesis that ergosterol homeostasis is a central contributor to the alkaline pH response of many fungal pathogens.
Translating basic investigations in the role of pH modulation in human disease into potential clinical applications has precedent in cancer biology. In mammalian cells, studies of pH regulation in tumor metastasis demonstrated an association between the pH within a tumor and the degree of tumor cell apoptosis, survival, and proliferation (73). The preference among certain malignant cells for more acidic external environments has prompted the exploration of "buffer therapy," in which site-directed pH modulation is used as an adjunctive therapy to limit tumor growth (74). This type of therapy is also effective against microbial infections that colonize the airways and intestines, such as Pseudomonas aeruginosa and Escherichia coli, respectively (75-77). If these interventions can be used against bacterial infections, one might imagine how similar pH modulation could be specifically applied to combat the acidic, necrotic core of many established invasive fungal infections, including cryptococcal lesions (57,78,79). Understanding pH-mediated microbial changes in various host microniches will allow for the development of optimized antifungal activity at the site of infection.

MATERIALS AND METHODS
Strains, media, and growth conditions. Strains generated and/or utilized in this study are shown in Table 1. Each mutant, reconstituted strain, and fluorescent strain were generated in the C. neoformans H99 MAT␣ genetic background and incubated in either yeast-peptone-dextrose medium (YPD) (1% yeast extract, 2% peptone, and 2% dextrose) or yeast nitrogen base medium (YNB). The pH 4, 5, 5.5, 6, 7, and 8 media were made by adding 150 mM HEPES buffer to YPD or YNB medium, adjusting the pH with concentrated HCl (for pH Ͻ5.5) or NaOH (for pH Ͼ5.5), prior to autoclaving. Medium was supplemented with 20% glucose following autoclaving unless otherwise noted. Cell wall stress phenotypes were assessed by growth on various stress medium agar plates as previously described (32). Congo red (0.5%) and NaCl (1.5 M) were added to YPD medium prior to autoclaving. Caffeine (1 mg/ml) and SDS (0.03%) were filter sterilized and added to YPD medium following autoclaving. Cobalt chloride plates were made by adding 7 mM (90.89 mg/liter) CoCl 2 solution to autoclaved YES medium (glucose, yeast extract, adenine, uracil, histidine, leucine, lysine, and agar) (80,81). Capsule induction and analyses were completed as previously described (32). Briefly, strains were incubated overnight in YPD medium and then diluted in tissue culture medium (CO 2 -independent tissue culture medium [TC]; Gibco) for 72 h with shaking at 37°C and then counterstained with India ink. The microaerophilic conditions were generated using a sealed chamber (BD GasPak) and two activated packs of GasPak EZ Campy container system (containing campylobacter) to reduce oxygen levels. YPD plates with serial dilutions of normally grown strains were placed in the chamber for 24 h at 37°C (microaerophilic) or outside the chamber for 24 h at 37°C (ambient air).
The ergosterol supplementation and growth curve analysis were conducted in a 96-well plate. Strains were incubated overnight (ϳ18 h) at 30°C with 150-rpm shaking. Cells were then pelleted and resuspended in either pH 4 or pH 8 synthetic complete medium buffered with McIlvaine's buffer (39). Resuspended strains were added to wells containing the same-pH synthetic complete medium with either 2 g/ml or 0.02 g/ml of ergosterol (Sigma)-Tween 80-ethanol (2-mg/ml stock as previously described in reference 82). Growth was then measured at an absorbance of 595 nm every 10 min for 42 h with shaking between readings and incubation at 30°C. Control wells containing vehicle alone (ethanol and Tween) were also measured in order to ensure that any growth rate change detected was due to the addition of ergosterol. One-way analysis of variance (ANOVA) and Dunnett's multiple-comparison test were run on the last time point under each condition compared to the pH 8-alone condition to determine statistical significance. The pH of the medium in the wells was tested following the experiment to ensure that the medium remained buffered. To generate the sre1Δ deletion and eGFP-Rim101 ϩ sre1Δ deletion and tagged deletion constructs, respectively, we performed the previously described double-joint PCR with split drug resistance marker method to make targeted gene deletions (15,83). In brief, we generated the following two PCR products: 5= flanking region of the target locus (1,000 bp) with a truncated drug resistance cassette and the remainder of the drug resistance cassette with the 3= flanking region of the target locus (1,000 bp). We then used biolistics to transform these two amplicons into either the wild-type C. neoformans strain (H99) or the C. neoformans strain that contains endogenously expressed GFP-Rim101 (84). Transformants were selected for the presence of the construct on YPD medium plus neomycin (NEO). To generate the fluorescently tagged His-GFP-Sre1 strain, we used In-Fusion (Clontech) to clone the SRE1 gene and terminator into the HGNAT (pCN19) plasmid, containing the GFP sequence and the nourseothricin (NAT) resistance marker (85). This plasmid was then biolistically transformed into the H99, wild-type (WT) strain. To generate the SRE1 reconstituted strain, we cloned the SRE1 gene and terminator into the pCH233 plasmid, containing the nourseothricin (NAT) resistance marker (86). This plasmid was then biolistically transformed into the sre1Δ (HEB5) strain. The primers used to generate each strain are listed in Table 2. Primers used to validate all sre1Δ mutants through Southern analysis (data not shown) are also listed in Table 2. Transformants were selected on YPD medium containing NAT (fluorescent strain) or NAT/NEO (reconstituted strain). Plasmids used in this study to amplify markers and clone new plasmids are listed in Table 3.
Microscopy. To analyze GFP-Rim101 localization in the WT, rim20Δ, and sre1Δ backgrounds, strains were incubated overnight (ϳ18 h) at 30°C with 150-rpm shaking. Cells were then pelleted and resuspended in either pH 4 or pH 8 synthetic complete medium buffered with McIlvaine's buffer. Strains were shaken at 150 rpm and 30°C for 60 min as this has been shown to be sufficient time to observe the nuclear localization of Rim101 in WT cells (15). Fluorescent images were captured using a Zeiss Axio Imager A1 fluorescence microscope equipped with an Axio-Cam MRM digital camera. Images were created using ImageJ software (Fiji) (87).
Protein extraction, immunoprecipitation, and Western blotting. Protein extracts were prepared in a similar manner to what was previously described (15). Briefly, strains were incubated for ϳ18 h at 30°C with 150-rpm shaking in YPD medium buffered to pH 4 or 5.5 with HEPES and HCl. Cells were then pelleted and resuspended in YPD medium buffered to pH 8 with HEPES and NaOH. These cells were incubated for 60 min and immediately pelleted and flash frozen on dry ice. Lysis was performed by bead beating (0.   Histone H3 promoter; GFP; SRE1 including terminator pCN19 This study pelleted through centrifugation at 15,000 rpm, 4°C, for 5 min, and the supernatant (cell lysate) was transferred (ϳ1 ml) to a new tube. For Western blotting assays assessing the presence of protein by probing for GFP, 50 l of lysate was saved as whole lysate and 25 l of GFP-trap (Chromotek) resin (equilibrated and resuspended in lysis buffer) was added to the remaining lysate. The lysates containing GFP-trap were incubated at 4°C for 2 h, rotating. Following incubation, lysates were spun down (2,500 ϫ g for 2 min at 4°C) and washed 3 times with detergent-free buffer containing 2ϫ protease inhibitors (Complete, Mini, EDTA-free; Roche), 1ϫ phosphatase inhibitors (PhosStop; Roche), and 1 mM phenylmethanesulfonyl fluoride (PMSF). GFP-trap resin was resuspended in 4ϫ NuPage lithium dodecyl sulfate (LDS) loading buffer and 10ϫ NuPage reducing agent. Western blotting assays were performed using a 4% to 12% NuPage BisTris gel. To probe and detect GFP-Rim101 and GFP-Sre1, immunoblots were incubated with an anti-GFP primary antibody (using a 1/10,000 dilution; Roche), followed by a secondary anti-mouse peroxidase-conjugated secondary antibody (using a 1/25,000 dilution; Jackson Laboratory). Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent; GE Healthcare). For Western blotting assays assessing the presence of cleaved and uncleaved Sre1 using the polyclonal anti-Sre1, lysates were prepared in the same way as previously described. Following lysis and initial centrifugation of the crude pellet, 500 l of lysates was precleared with 30 l protein A-agarose (Sigma) and rotated for 1 h at 4°C. Lysates were incubated with 5 l of anti-Sre1 polyclonal antibody (generously given to us by the Espenshade lab [21]) for 1 h. Protein A (60 l/sample) was washed twice in lysis buffer and resuspended in equal volumes. Equilibrated protein A was then added to each lysate and incubated at 4°C for 1 h, rotating. Following incubation, lysates were spun down (2,500 ϫ g for 2 min at 4°C) and washed twice with lysis buffer, once with lysis buffer plus 1 M NaCl, and twice with lysis buffer. Protein A resin was then resuspended in 4ϫ NuPage lithium dodecyl sulfate (LDS) loading buffer and 10ϫ NuPage reducing agent. Western blot assays were performed using a 3% to 8% NuPage Tris-acetate gel, with Tris-acetate running buffer. To probe for and detect Sre1, immunoblots were incubated in anti-Sre1 primary antibody (using a 1/200 dilution [21]) and then in anti-rabbit peroxidase-conjugated secondary antibody (using a 1/50,000 dilution; Jackson Laboratory). Proteins were detected in the same way as described above.
Cell wall staining and flow cytometry. For chitin and exposed chitin detection, cell wall staining with wheat germ agglutinin (WGA) and calcofluor white (CFW) was assessed as previously described (32). Briefly, overnight YPD cultures were diluted 1:10 in CO 2 -independent liquid medium and incubated (ϳ18 h) at 37°C with 150-rpm shaking. Cells were stained with 100 g/ml of fluorescein isothiocyanate (FITC)-conjugated WGA and 25 g/ml CFW and incubated in the dark for 35 min and 10 min, respectively. Quantitative analysis using ImageJ software was performed as previously described (32,41).
For flow cytometry analysis, cells were incubated similarly as described above and fixed with 3.7% formaldehyde for 5 min at room temperature. Cells were then slowly pelleted and washed twice with phosphate-buffered saline (PBS). Cells were stained with 100 g/ml FITC-conjugated wheat germ agglutinin (WGA; Molecular Probes). Cells stained with WGA were incubated in the dark at room temperature for 35 min. Cells were then slowly pelleted and washed twice with PBS. Cells from each strain were stained and resuspended in PBS at a concentration of 10 7 cells/ml. Cells at 10 6 /ml were submitted to the Duke Cancer Institute Flow Cytometry Shared Resource for analysis using a BD FACSCanto II flow cytometer. Data were analyzed by FlowJo v10.6.1 software (FlowJo, LLC). Unstained cells were used as negative controls, and positive events were gated in the forward scatter/side scatterplots and represented as histograms. Geometric means were calculated based on the mean fluorescence intensity (x axis of histogram) of all cells quantified for each strain (y axis of histogram).
Macrophage survival assay. J774A.1 cells were incubated in a humidified 37°C incubator with 5% CO 2 , passaged twice weekly, and kept in tissue culture flasks in 20 to 25  . Survival of C. neoformans strains within alveolar macrophage-like J744A.1 cells was assessed by aliquoting 100 l of 10 5 viable cells into a 96-well plate, avoiding edges as previously described (88). The plates were incubated overnight in a 37°C incubator with 5% CO 2 . Macrophages were then activated with 10 nM phorbol myristate acetate (PMA) and incubated at 37°C, 5% CO 2 , for 1 h. Fungal cells were incubated overnight (ϳ18 h) at 30°C with 150-rpm shaking. Cells were then pelleted, washed twice in PBS, and resuspended in macrophage medium. Fungal cells (10 6 cells/ml) were opsonized with monoclonal antibody (Mab) 18B7 (1 g/ml) for 1 h at 37°C. Cell concentrations were verified with quantitative culture. Macrophage medium was removed from the 96-well plate, and 100 l of opsonized fungal cells was added to each well. The cocultures were incubated for 1 h at 37°C incubator with 5% CO 2 . Each well was then washed 3 times with PBS to remove extracellular yeast. One hundred microliters of macrophage medium was added to each well and incubated for 24 h at 37°C with 5% CO 2 . Following incubation, macrophage killing was determined by adding 200 l sterile distilled water (dH 2 O) to each well, incubating at room temperature for 5 min, and assessing by quantitative cultures. One-way ANOVA and Tukey's multiple-comparison tests were run to assess statistical significance between fungal cell survival percentages. Six biological replicates of each strain were analyzed.
RNA-sequencing preparation and analyses. WT and sre1Δ cells were incubated at 30°C with 150-rpm shaking in YPD medium to mid-logarithmic phase. Approximately 1 ϫ 10 9 cells from each strain were pelleted and resuspended in YPD medium buffered to pH 4 or pH 8 and incubated at 30°C for 90 min with 150-rpm shaking. All cells were pelleted, flash frozen on dry ice, and lyophilized overnight. This experiment was conducted with six biological replicates for the WT strain and the sre1Δ strain under both pH 4 and pH 8 conditions (24 samples total). RNA was isolated using the Qiagen RNeasy Plant minikit with optional on-column DNase digestion (Qiagen, Valencia, CA). RNA quantity and quality were measured using the Agilent 2100 Bioanalyzer. The NEBNext poly(A) mRNA magnetic isolation module was used to enrich for mRNA, and the NEBNext Ultra II directional RNA library prep kit for Illumina was used to prepare libraries (New England Biolabs, Ipswich, MA). Libraries were submitted to the Duke Sequencing and Genomic Technologies Shared Resource for sequencing on the Illumina NextSeq 500 with 75-bp, single-end reads.
Reads were mapped to the C. neoformans H99 reference genome (obtained from NCBI, accessed July 2019) using STAR alignment software (89). Differential expression analyses were performed in R using an RNA᎑Seq Bioconductor workflow (90,91) followed by the DESeq2 package with a false-discovery rate (FDR) of 5% (92). Genes were considered statistically differentially expressed if they had an adjusted P value of Ͻ0.05.
A modified Gene Ontology-term (GO-term) analysis using the FungiDB database was performed to identify genes that were significantly regulated in a given process as previously reported (15,93). The differentially expressed genes in each category were determined based on two criteria: P value of Ͻ0.05 and base mean value of Ͼ20. Further differentiation was made based on the log 2 fold change values. For the sre1Δ versus wild-type data set, we used a log 2 fold change of Ϯ1. For the positively regulated genes in the wild-type pH 4 versus pH 8 data set, we used a log 2 fold change of 1, and for the negatively regulated genes in the wild-type data set, we used a log 2 fold change of Ϫ3 due to the large amount of genes in this set. Fold change graphs were generated in GraphPad Prism (GraphPad Prism version 8.00 for Mac, GraphPad Software, San Diego, CA, USA), and Seaborn was used to visualize the DESeq2 results in a volcano plot (94). A complete list of the transcriptome sequencing (RNA-seq) data sets containing differentially expressed genes in each strain and associated with the appropriate GO-term category can be found in Table S1 in the supplemental material.
Antifungal susceptibility tests. For fluconazole and amphotericin B (AMB) Etest assays and pyrifenox disc diffusion, fungal cells were incubated overnight (ϳ18 h) at 30°C with 150-rpm shaking in YPD. Cells were normalized to an optical density at 600 nm (OD 600 ) of 0.6 and diluted 1:10 in PBS, and 100 l was plated to either YPD pH 5.5 or YPD pH 8 agarose plates. For the fluconazole and AMB Etest assay, an Etest strip (bioMérieux) containing a gradient of drug concentrations was placed on top of the plated fungal lawn. Plates were then incubated at 30°C for 72 (AMB) and 120 (fluconazole) h. Pyrifenox susceptibility was assessed by standard disc diffusion assays using 5 l pyrifenox (Sigma-Aldrich; CAS number 88283-41-4; final concentration of 1.2 g/ml). Plates were then incubated at 30°C for 72 h. Zones of inhibition were determined as a surrogate of antifungal activity.
MIC testing of AMB against a pH gradient was performed by broth microdilution. AMB resuspended in dimethyl sulfoxide (DMSO) was serially diluted in synthetic complete medium buffered to pH 4, 5, 6, 7, or 8 with McIlvaine's buffer in a 96-well plate with the highest concentration being 3.2 g/ml. Fungal cells were incubated overnight (ϳ18 h) at 30°C with 150-rpm shaking in YPD. Cells were then normalized and diluted in synthetic complete medium buffered to pH 4, 5, 6, 7, or 8 with McIlvaine's buffer and added to the corresponding pH well containing AMB. Plates were incubated at 30°C for 48 h, and the MIC was determined to be the lowest concentration of drug that led to no fungal cell growth.

SUPPLEMENTAL MATERIAL
Supplemental material is available online only.

ACKNOWLEDGMENTS
We acknowledge the Duke Sequencing and Genomic Technologies Shared Resource for their assistance with the various projects in this study. We thank Josh Granek and the other staff of the High Throughput Sequencing Course held through the Department of Biostatistics and Bioinformatics at Duke University for their assistance with our RNA sequencing library prep and analysis. We thank the Espenshade Laboratory for their generous gifting of the anti-Sre1 polyclonal antibody. We also thank the Madhani Laboratory for the erg4Δ and cnag_00490Δ deletion strains available through the Fungal Genetics Stock Center. We thank Max Moskovitz for his assistance with phenotyping sterol-related deletion strains. We thank the Heitman Laboratory for sharing the various Candida deletion strains.
We acknowledge our own NIH funding (R01 AI074677, 1F31A140427-01A1, P01 AI104533) that made these studies possible. We also thank the NIH funding