Genome Sequence of Porphyromonas gingivalis Strain 381

ABSTRACT Porphyromonas gingivalis is associated with both oral and systemic diseases. Strain-specific P. gingivalis invasion phenotypes do not reliably predict disease presentation during in vivo studies. Here, we present the genome sequence of 381, a common laboratory strain, with a single contig of 2,378,872 bp and a G+C content of 48.36%.

P. gingivalis strain 381 was obtained from F. Macrina (Virginia Commonwealth University) and grown as previously described (21). Genomic DNA was obtained using the Wizard gDNA purification kit (Promega) and processed to generate shotgun and 3-kb paired-end libraries, which were sequenced using the 454 Life Sciences GS-20 instrument (22) (Roche). 806,578 reads of 123,742,668 bp, with an average read length of 153 bp, were generated.
The annotated P. gingivalis 381 genome was compared to P. gingivalis strains W83, ATCC 33277, and TDC60 using RAST (27) and IMG-ER (28). All-to-all BLASTp comparisons of predicted protein sequences showed that 381 possesses 64 strain-specific CDSs, all annotated as hypothetical proteins. Of note, 381 is a close relative of ATCC 33277 based on genome clustering analysis, and the gene order is nearly identical between 381 and ATCC 33277, except three minor differences due to inversion, duplication, or deletion of transposable elements.
The availability of the 381 genome enables exploration of how genomic differences among P. gingivalis strains offer widely different in vitro phenotypes, but may not confer competitive advantage in an animal model of infection.
Accession number(s). This genome sequencing project was deposited in GenBank under accession number CP012889. The version described is the first version.

ACKNOWLEDGMENTS
This study was supported by a University of Florida College of Dentistry Multi-Investigator Pilot Program Project Grant (to A.P.F.), as well as National Institute for Dental and Craniofacial Research grant DE013545-07S1 (to A.P.F.) and contract Y1-DE-6006-02 (to Los Alamos National Laboratory).
We thank the staff of the University of Florida Interdisciplinary Center for Biotechnology Research, especially Regina Shaw, for excellent technical assistance. We declare no conflict of interest.