Draft Genome Sequence of Streptomyces sp. Strain JV178, a Producer of Clifednamide-Type Polycyclic Tetramate Macrolactams

ABSTRACT Here, we report the draft genome sequence of Streptomyces sp. JV178, a strain originating from Connecticut (USA) garden soil. This strain produces the polycyclic tetramate macrolactam compounds clifednamides A and B. The draft genome contains 10.65 Mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.

M embers of the Streptomyces genus of Gram-positive filamentous bacteria produce diverse secondary metabolites, including two-thirds of clinical antibiotics (1). Streptomyces genomes have been shown to contain a rich repertoire of small-moleculeencoding biosynthetic loci (2). A high proportion of sequenced Streptomyces strains encode the biosynthesis of polycyclic tetramate macrolactams (PTMs) (3). Streptomyces sp. strain JV178 was isolated from a Connecticut (USA) garden soil sample during a screen to discover new PTM compounds (4). We have sequenced its genome to further understand clifednamide-type PTM biogenesis.
Strain JV178 was grown in Trypticase soy broth supplemented with 0.6% glycine at 28°C. Genomic DNA was extracted by phenol-chloroform extraction as described elsewhere (5). Sequencing was performed on a MiSeq platform (2 ϫ 301-bp reads [Illumina, Inc., San Diego, CA, USA]) on a paired-end library prepared using a highthroughput library preparation kit (Kapa Biosystems). Adapter sequences and lowquality reads (quality score Ͻ 0.05) were removed using CLC Genomics Workbench (CLC Bio-Qiagen, Aarhus, Denmark). The trimmed reads totaled 3,064 Mb, corresponding to approximately 287-fold coverage. De novo genome assembly was performed in CLC Genomics Workbench, and resulting contigs having less than 200 bp or 3ϫ average coverage were discarded. The resulting draft genome contains 10,650,097 bp in 759 contigs (N 50 , 540,451 bp), with a GϩC content of 71.0%. Gene prediction and annotation by the Rapid Annotations using Subsystems Technology version 2.0 pipeline (6, 7) predicted 9,045 protein coding sequences and 126 RNA genes.
Accession number(s). The draft genome sequence of Streptomyces sp. strain JV178 was deposited in DDBJ/ENA/GenBank under the accession number PEKU00000000. The version described in this paper is the first version, PEKU01000000.

ACKNOWLEDGMENTS
The genome of Streptomyces sp. strain JV178 was sequenced with the help of Chris Shaffer and the McDonnell Genome Institute at Washington University in St. Louis. We also thank Michael Guzman (WUSTL) for assistance and advice with the assembly.
This study received no specific grant from any funding agency in the public, commercial, or not-for-profit sector.