Draft Genome Sequence of an Obligate and Moderately Halophilic Bacterium, Thalassobacillus devorans Strain MSP14, the First Draft Genome of the Genus Thalassobacillus

We report the 3.93-Mbp first draft genome sequence of a species of the genus Thalassobacillus, Thalassobacillus devorans strain MSP14, a moderate but obligate halophile, isolated from a salt crystallizer of the Little Rann of Kutch, India. Exploring the genome of this organism will facilitate understanding the mechanism(s) of its obligate halophilism.

salt crystallizers of the Rann of Kutch, Gujarat, India, have been sequenced with a view to understanding the mechanism(s) of osmotolerance (1-4). Thalassobacillus devorans strain MSP14 (16S rRNA, GenBank accession no. JX518269), an obligate but moderately halophilic bacterium, was isolated from a salt crystallizer of the Little Rann of Kutch, India. It grows optimally at a concentration of 7.5% NaCl (range, 5 to 15%) in medium at 37°C and pH 7.5. The present genome of MSP14 was sequenced to understand the mechanism(s) of obligate, but moderate, halophilism.
By use of the Roche 454 genome sequencer (GS FLX), the genome of Thalassobacillus devorans strain MSP14 (GϩC content of 42.97%) was sequenced at Macrogen, Inc., South Korea, through Sequencher Tech Pvt., Ltd., Ahmedabad, India. Both shotgun and 3-kb mate-paired library sequencing were performed. Whereas sequencing of shotgun libraries generated 787,155 reads of 439,717,712 bases (average read length of 558 bp), mate-paired libraries generated 140,683 and 131,751 reads of 62,893,040 and 57,724,648 bases, respectively, with average read lengths of 447 and 438 bp, respectively.
De novo assembly of the reads using GS De Novo Assembler v 2.6 (5) gave approximately 138-fold coverage with 9 scaffolds of 3,935,133 bp and 32 contigs of 3,923,021 bp with average lengths of 437,237 bp and 122,594 bp, respectively. An N 50 scaffold length of 1,214,989 bp (smallest, 2,007 bp and largest, 1,848,831 bp) and an N 50 contig length of 260,182 bp (smallest, 1,596 bp and largest 467,343 bp) were obtained. All assembly data were deposited in the DDBJ/EMBL/GenBank nucleotide sequence database.
We are exploring the genome of Thalassobacillus devorans MSP14 further to unravel the mechanism(s) of its obligate halophilism.
Nucleotide sequence accession numbers. This whole genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number AWXW00000000. The version described in this paper is version AWXW01000000.

ACKNOWLEDGMENTS
The work was carried out in the subproject "Diversity analysis of Bacillus and other predominant genera in extreme environments and its utilization in agriculture" of the National Agricultural Innovation Project (NAIP) and "Exploring bacterial and archaebacterial diversity in the