Complete Genome Sequence of Bacillus megaterium Podophage Pavlov

Bacillus megaterium is a large spore-forming bacterium found widely in the environment. Phages infecting B. megaterium can be used as genetic tools to expand the array of uses of B. megaterium in research and industry. Here, we present the complete genome of Pavlov, a podophage infecting B. megaterium.

field of microbiology. Due to its large size, secretion ability, and stable plasmid replication system, it has become a workhorse for recombinant protein production in the biotechnology industry (1). Recently, the systems metabolic engineering of B. megaterium has gained interest (2). Bacteriophages have a broad array of applications (3) and may play a role in developing the full potential of industrial B. megaterium strains. To that end, here we describe the novel B. megaterium podophage Pavlov.
Bacteriophage Pavlov was isolated from a soil sample collected in College Station, Texas, USA, based on its ability to grow on the asporogenic strain B. megaterium KM (ATCC 13632). Phage DNA was sequenced in an Illumina MiSeq 250-bp paired-end run with a 550-bp insert library at the Genomic Sequencing and Analysis Facility at the University of Texas (Austin, TX, USA). Qualitycontrolled, trimmed reads were assembled to a single contig of circular assembly at 25.4-fold coverage using SPAdes version 3.5.0. The contig was confirmed to be complete by PCR using primers that face the upstream and downstream ends of the contig. Products from the PCR amplification of the junctions of concatemeric molecules were sequenced by Sanger sequencing (Eton Bioscience, San Diego, CA, USA). Genes were predicted using GeneMarkS (4) and corrected using software tools available on the Center for Phage Technology (CPT) Galaxy instance (https://cpt .tamu.edu/galaxy-pub/). The morphology of Pavlov was determined using transmission electron microscopy performed at the Texas A&M University Microscopy and Imaging Center.
Replication, biosynthesis, packaging, transcriptional regulation, morphogenesis, lysis genes, and an HNH-homing endonuclease were identified in Pavlov. Three DNA-binding proteins, presumably transcriptional regulators, were found to contain lambda Cro/CI-type helix-turn-helix domains. Additionally, the presence of a plasmid replication/relaxation protein suggests that Pavlov may be a temperate phage. Like other phages of this group, Pavlov encodes an FtsK/SpoIIIE homolog. In Gram-positive bacteria, SpoIIIE is an ATPase that translocates DNA across the septal membrane of the sporulating mother cell into the forespore, although its role in the phage life cycle is unknown (14). Pavlov encodes a SigF-like sporulation-related sigma factor, indicating that the phage might influence the vegetative growth and sporulation of the host (15).
Nucleotide sequence accession number. The genome sequence of phage Pavlov was contributed to GenBank under the accession number KT001911.

ACKNOWLEDGMENTS
This work was supported primarily by funding from award number EF-0949351, "Whole Phage Genomics: A Student-Based Approach," from the National Science Foundation. Additional support came from the Center for Phage Technology, an Initial University Multidisciplinary Research Initiative supported by Texas A&M University and Texas AgriLife, and from the Department of Biochemistry and Biophysics.
We are grateful for the advice and support of the CPT staff. This announcement was prepared in partial fulfillment of the requirements for BICH464 Phage Genomics, an undergraduate course at Texas A&M University.