Complete Genome Sequence of Spiroplasma floricola 23-6T (ATCC 29989), a Bacterium Isolated from a Tulip Tree (Liriodendron tulipifera L.)

ABSTRACT Spiroplasma floricola 23-6T (ATCC 29989) was isolated from the flower surface of a tulip tree (Liriodendron tulipifera L.). Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology and the comparative genomics among Spiroplasma species.

T he strain Spiroplasma floricola 23-6 T was isolated from a tulip tree (Liriodendron tulipifera L.) flower collected in the state of Maryland in the United States (1)(2)(3). This bacterium was shown to be pathogenic to larvae of the greater wax moth (Galleria mellonella) in an artificial infection experiment (4). To facilitate future investigation of the biology of this bacterium, as well as to improve the taxon sampling of available Spiroplasma sequences for comparative genomics and evolutionary studies (5), we determined its complete genome sequence.
The strain was acquired from the American Type Culture Collection (catalog number ATCC 29989). The freeze-dried sample was processed according to the manufacturer's instruction and cultured in M1D medium (6) prior to DNA extraction using the Wizard Genomic DNA purification kit (Promega, USA). PCR and Sanger sequencing were performed to verify that the 16S rRNA gene sequence matched the reference record (GenBank accession number AY189131) (7).
The complete genome sequence of Spiroplasma floricola 23-6 T consists of one circular chromosome that is 1,284,130 bp in size with 24.3% GϩC content; no plasmid was found. The first version of annotation includes one set of 16S-23S-5S rRNA genes, 29 tRNA genes (covering all 20 amino acids), 1,122 protein-coding genes, 8 pseudo-genes, and 1 CRISPR locus (chromosomal positions 565,140 to 565,547, containing five spacers).
Accession number(s). The complete genome sequence of Spiroplasma floricola 23-6 T has been deposited at DDBJ/EMBL/GenBank under the accession number CP025057.

ACKNOWLEDGMENTS
The Illumina paired-end sequencing service was provided by Yourgene Bioscience (New Taipei City, Taiwan). The Illumina mate-pair sequencing service was provided by the Genomic Technology Core Facility (Institute of Plant and Microbial Biology) and the Genomics Core Facility (Institute of Molecular Biology) at Academia Sinica (Taipei, Taiwan).
The funding for this project was provided by the Institute of Plant and Microbial Biology at Academia Sinica and the Ministry of Science and Technology of Taiwan (NSC 101-2621-B-001-004-MY3 and MOST 104-2311-B-001-019) to C.-H.K. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.