Complete Draft Genome Sequence of Escherichia coli JF733

Escherichia coli JF733 is a strain with a long history in research on membrane proteins and processes. However, tracing back the strain development raises some questions concerning the correct genotype of JF733. Here, we present the complete draft genome of E. coli JF733 in order to resolve any remaining uncertainties.

coli Genetic Stock Center (CGSC, New Haven, CT, USA). The strain has a long history, and CGSC tries to collect and update all information about their available strains and their characteristics. Sequencing of E. coli JF733 enables the clarification of remaining uncertainties and gives new insight into the genetic background of E. coli JF733.
Initial studies using E. coli JF733 were focused on functional studies of outer membrane proteins and porins (3)(4)(5) as well as on their contribution to bacteriophage and colicin sensitivity (2, 6). Furthermore, production and secretion of recombinant proteins like anti-␣TF using JF733 were described in 2008 (7).
To resolve remaining uncertainties concerning the JF733 genotype for further research, the draft genome sequence of E. coli JF733 was established on the Illumina MiSeq system as recently described for other microorganisms (8)(9)(10). A paired-end sequencing run (2 ϫ 300-bp) yielded 2,266,996 reads with a total size of 646.20 Mb. Assembly using the GS de novo assembler version 2.8 resulted in 107 contigs and 58 scaffolds for the JF733 draft genome. Annotation of the genome was accomplished within the GenDB platform (11). The chromosome has a size of 4,518,620 bp with a GϩC content of 50.78%. In total, 4,218 coding sequences, 70 tRNA genes, and 3 species of rRNA genes were identified by the gene and RNA prediction tools.
Sequencing of the E. coli JF733 genome and comparison to E. coli W3110 (GenBank: AP009048.1) confirmed most of the indicated mutations and resulted in a specification of three affected genes, namely, hisD, ilvD, and xylR. No mutations in ompC, metB (and metC), and cycB were detected. Whereas the latter is not annotated for E. coli W3110, a potential deletion within tsx could not be verified with certainty using the obtained data. Although no mutation within ompC was detected, a base-pair substitution located in the Ϫ10 promoter region 90 bp upstream of ompC (12) was found, which could explain the described lack of protein Ib (2).
Nucleotide sequence accession numbers. The E. coli JF733 draft genome sequence was deposited in the EMBL database under the accession numbers FBSE01000001 to FBSE01000058.

ACKNOWLEDGMENTS
The bioinformatics support of the BMBF-funded project "Bielefeld-Gießen Center for Microbial Bioinformatics-BiGi" (grant number 031A533) within the German Network for Bioinformatics Infrastructure (de.NBI) is gratefully acknowledged.
We acknowledge support for the Article Processing Charge by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.