Draft Genome Sequence of Marinomonas fungiae Strain AN44T (JCM 18476T), Isolated from the Coral Fungia echinata from the Andaman Sea

ABSTRACT Marinomonas fungiae strain AN44T was isolated from mucus of the coral Fungia echinata. Optimum growth occurs at 3 to 5% NaCl. The draft genome is 4.2 Mb, with 3,776 protein-coding genes. It harbors genes for the degradation of aromatic compounds, such as quinate, ferulate, p-coumarate, protocatechuate, and p-hydroxyphenylacetate.

M arinomonas fungiae strain AN44 T (JCM 18476 T ) is an aerobic, Gram-negative, motile, and rod-shaped bacterium belonging to the class Gammaproteobacteria and was isolated from mucus of the coral Fungia echinata from the Andaman Sea (1). The draft genome sequence will help in understanding the plasticity and diversity of metabolic pathways for the degradation of aromatic compounds/pollutants in marine bacteria. Further, the strain harbors two unique mobile genetic elements belonging to the SXT integrating conjugative element (ICE) family (2).
The draft genome sequence of M. fungiae strain AN44 T was generated at the DOE Joint Genome Institute (JGI) using the Illumina HiSeq 2000 platform (3). An Illumina standard shotgun library was constructed and sequenced using the Illumina HiSeq 2000 platform, which generated 10,961,766 reads totaling 1,655.2 Mb. The filtered Illumina reads were assembled using the Velvet (4), wgsim (https://github.com/lh3/ wgsim), and AllPaths-LG (5) tools. The final draft assembly contains 42 contigs in 36 scaffolds, totaling 4.2 Mb, with an input read coverage of 268.9-fold. The largest and N 50 contigs are 554.8 kb and 268.5 kb, respectively, with a GϩC content of 46.2%.
Further, putative genes or gene clusters for peripheral aromatic compound degradation pathways of quinate, ferulate, and p-coumarate, along with a single operonic gene cluster for central aromatic compound degradation via the protocatechuate meta-cleavage pathway, were found in the genome. Additionally, a single operonic gene cluster for the degradation of p-hydroxyphenylacetate was found.
Accession number(s). This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LIQF00000000. The version described in this paper is the first version, LIQF01000000, and consists of sequences LIQF01000001 to LIQF01000036.

ACKNOWLEDGMENTS
This work was supported in part by the funding received by S.K.D. from the Ministry of Earth Sciences, Government of India (MoES/11-MRDF/1/59/P/08). P.K. and J.B. acknowledge the University Grant Commission and the Department of Biotechnology (DBT), Government of India, New Delhi, respectively, for providing research fellowships. This work was also supported by the Office of Science of the U.S. Department of Energy under contract DE-AC02-05CH11231.