Draft Genome Sequence of Mesorhizobium sp. UFLA 01-765, a Multitolerant, Efficient Symbiont and Plant Growth-Promoting Strain Isolated from Zn-Mining Soil Using Leucaena leucocephala as a Trap Plant.

We report the 7.4-Mb draft genome sequence of Mesorhizobium sp. strain UFLA 01-765, a Gram-negative bacterium of the Phyllobacteriaceae isolated from Zn-mining soil in Minas Gerais, Brazil. This strain promotes plant growth, efficiently fixes N2 in symbiosis with Leucaena leucocephala on multicontaminated soil, and has potential for application in bioremediation of marginal lands.

ising candidates for use in reclamation, particularly in phytoremediation.
We isolated an efficient N 2 -fixing and plant growth-promoting bacterium from Zn-contaminated mining soil in Minas Gerais, Brazil. Identified as a Mesorhizobium sp. by partial 16S rRNA gene sequencing (99% identity to Mesorhizobium sp. strain CCANP87, Genbank accession no. HF931067) (1), strain UFLA 01-765 was selected for genome sequencing.
Genomic DNA was isolated from stationary-phase cells using a DNeasy blood and tissue kit (Qiagen, Venlo, the Netherlands), treated with RNase I and purified by phenol:chloroform extraction. A library was constructed according to Thijs et al. (2) prior to sequencing on an Ion Torrent PGM (Life Technologies Inc., Carlsbad, CA).
Mesorhizobium sp. UFLA 01-765 is a promising inoculant for L. leucocephala to stimulate revegetation of Zn-and Cd-contaminated sites, and it is a candidate as a type strain for the genus in studies of chemoautotrophic growth in rhizobia.
Nucleotide sequence accession numbers. This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank un-der the accession no. LPWA00000000. The version described in this paper is version LPWA01000000.

ACKNOWLEDGMENTS
This work was supported by a PhD grant to Wesley Melo Rangel for a doctoral training sandwich abroad (BEX: 13079/2013-01) from the Brazilian Commission for Improvement of Higher Education Staff (CAPES), and financial resources from the National Council for the Scientific and Technological Development (CNPq), the Commission for Improvement of Higher Education Staff (CAPES) and the Foundation for Research of the State of Minas Gerais (FAPEMIG). This work has also been financially supported by the Hasselt University Methusalem project 08M03VGRJ. We gratefully acknowledge funding support from the Rede de Pesquisas em Áreas Afetadas por Mineração (RECUPERAMINA) through its coordinator Luiz R. G. Guilherme.

FUNDING INFORMATION
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.