Genome Sequence of Porphyromonas gingivalis Strain A7A1-28

ABSTRACT Porphyromonas gingivalis is an oral opportunistic pathogen. Sequenced P. gingivalis laboratory strains display limited diversity in antigens that modulate host responses. Here, we present the genome sequence of A7A1-28, a strain possessing atypical fimbrillin and capsule types, with a single contig of 2,249,024 bp and a G+C content of 48.58%.

The genome of P. gingivalis A7A1-28 has approximately 94-fold coverage and contains a single contig of 2,249,024 bp (GϩC content of 48.58%). A total of 1,982 genes were annotated, which included 1,915 predicted coding sequences (CDSs), 53 tRNAs, 12 rRNAs, and one tmRNA. There are 229 subsystems in the genome. Further, 191 protein metabolism, 128 cofactors, vitamins, prosthetic groups, and pigments, 65 RNA metabolism, 94 DNA metabolism, 99 carbohydrate, and 17 membrane transport subsystem features were observed.
The annotated P. gingivalis A7A1-28 genome was compared to P. gingivalis strains W83, ATCC 33277, and TDC60 using RAST (37) and IMG-ER (38). All-to-all BLASTP comparisons of predicted protein sequences showed that A7A1-28 possesses 119 strain-specific CDSs, of which 98 are annotated as hypothetical proteins. Further, A7A1-28 contains a variety of mobile genetic elements, including seven Bacteroides conjugative transposons absent from W83, ATCC 33277, and TDC60. Genome synteny analysis revealed that the gene order in A7A1-28 resembles that of A7436 and AJW4, suggesting that local mutations may generate unique phenotypes observed in A7A1-28.
The availability of the A7A1-28 genome aids investigators in efforts to decipher interactions between P. gingivalis and host tissue, which are critical to homeostasis in the subgingival microbiome.
Accession number(s). This genome sequencing project was deposited in GenBank under the accession number CP013131. The version described is the first version.

ACKNOWLEDGMENTS
This study was supported by a University of Florida College of Dentistry Multi-Investigator Pilot Program project grant (to A.P.F.), as well as National Institute for Dental and Craniofacial Research grant DE013545-07S1 (to A.P.F.), and contract Y1-DE-6006-02 (to Los Alamos National Laboratory). We thank the staff of the University of Florida Interdisciplinary Center for Biotechnology Research, especially Regina Shaw, for excellent technical assistance.