Development of the Embryotoxicity Test using the Hybrid 3D Cultivation Technique of Human Hepatocytes and a Mouse ES cells - Use of Novel Feeder

Koichi IMAI; Kazuaki NAKAMURA; Akito TANOUE; Kazuhiko SUESE; Yoshitomo HONDA; Fumiya OGAWA; Chiaki WATANABE; Hideo OHMUKAI; Hiromasa TAKASHIMA

doi:10.11223/jarde.11.51

ORIGINAL ARTICLES

Development of the Embryotoxicity Test using the Hybrid 3D Cultivation Technique of Human Hepatocytes and a Mouse ES cells - Use of Novel Feeder

Koichi IMAI, Kazuaki NAKAMURA, Akito TANOUE, Kazuhiko SUESE, Yoshitomo HONDA, Fumiya OGAWA, Chiaki WATANABE, Hideo OHMUKAI, Hiromasa TAKASHIMA

Author information

Koichi IMAI
Department of Biomaterials, Osaka Dental University, Osaka, Japan
Kazuaki NAKAMURA
Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
Akito TANOUE
Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
Kazuhiko SUESE
Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
Yoshitomo HONDA
Institute of Dental Research,
Fumiya OGAWA
Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
Chiaki WATANABE
Hatano Research. Institute, FDSC, Kanagawa, Japan
Hideo OHMUKAI
Hatano Research. Institute, FDSC, Kanagawa, Japan
Hiromasa TAKASHIMA
Ina Research Inc., Nagano, Japan

Keywords: human hepatocytes, uterus cells, oviduct cells, EL M3 cells, feeder cells, MEF

JOURNAL FREE ACCESS

2013 Volume 11 Issue 1 Pages 51-56

DOI https://doi.org/10.11223/jarde.11.51

Details

Abstract

The teratogenicity of thalidomide was demonstrated by a modified Embryonic Stem Cell (EST) method using a hybrid culture with human hepatocytes. Six kinds of primary cells derived from the mouse uterus or oviduct were used as feeder layers. As a result, the beating rate due to EL-M3 cell differentiation was slightly lower for some feeder layers after the addition of thalidomide. However, there was no significant difference due to the addition of thalidomide. This may be explained by the presence of atypical cells in the feeder cells. The results will be further analyzed.

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