Exploring cytoplasmic dynamics in zebrafish yolk cells by single particle tracking of fluorescent nanodiamonds

Cheng-Chun Chang; Bailin Zhang; Che-Yu Li; Chih-Chien Hsieh; Guillaume Duclos; François Treussart; Huan-Cheng Chang

doi:10.1117/12.907181

24 February 2012 Exploring cytoplasmic dynamics in zebrafish yolk cells by single particle tracking of fluorescent nanodiamonds

Cheng-Chun Chang, Bailin Zhang, Che-Yu Li, Chih-Chien Hsieh, Guillaume Duclos, François Treussart, Huan-Cheng Chang

Author Affiliations +

Proceedings Volume 8272, Advances in Photonics of Quantum Computing, Memory, and Communication V; 827205 (2012) https://doi.org/10.1117/12.907181
Event: SPIE OPTO, 2012, San Francisco, California, United States

Abstract

Fluorescent nanodiamonds (FNDs) have recently developed into an exciting new tool for bioimaging applications. The material possesses several unique features including high biocompatibility, easy bioconjugation, and perfect photostability, making it a promising optical nanoprobe in vitro as well as in vivo. This work explores the potential application of this novel nanomaterial as a photostable, nontoxic tracer in vivo using zebrafish as a model organism. We introduced FNDs into the yolk of a zebrafish embryo by microinjection at the 1-cell stage. Movements of the injected particles were investigated by using single particle tracking techniques. We observed unidirectional and stop-and-go traffic as part of the intricate cytoplasmic movements in the yolk cell. We determined a velocity in the range of 0.19 - 0.40 μm/s for 40 particles moving along with the axial streaming in the early developmental stage (1 to 2 hours post fertilization) of the zebrafish embryos.

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Cheng-Chun Chang, Bailin Zhang, Che-Yu Li, Chih-Chien Hsieh, Guillaume Duclos, François Treussart, and Huan-Cheng Chang "Exploring cytoplasmic dynamics in zebrafish yolk cells by single particle tracking of fluorescent nanodiamonds", Proc. SPIE 8272, Advances in Photonics of Quantum Computing, Memory, and Communication V, 827205 (24 February 2012); https://doi.org/10.1117/12.907181

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