PharmaSat: drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite

Antonio J. Ricco; Macarena Parra; David Niesel; Matthew Piccini; Diana Ly; Michael McGinnis; Andrzej Kudlicki; John W. Hines; Linda Timucin; Chris Beasley; Robert Ricks; Michael McIntyre; Charlie Friedericks; Michael Henschke; Ricky Leung; Millan Diaz-Aguado; Christopher Kitts; Ignacio Mas; Mike Rasay; Elwood Agasid; Ed Luzzi; Karolyn Ronzano; David Squires; Bruce Yost

doi:10.1117/12.881082

14 February 2011 PharmaSat: drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite

Antonio J. Ricco, Macarena Parra, David Niesel, Matthew Piccini, Diana Ly, Michael McGinnis, Andrzej Kudlicki, John W. Hines, Linda Timucin, Chris Beasley, Robert Ricks, Michael McIntyre, Charlie Friedericks, Michael Henschke, Ricky Leung, Millan Diaz-Aguado, Christopher Kitts, Ignacio Mas, Mike Rasay, Elwood Agasid, Ed Luzzi, Karolyn Ronzano, David Squires, Bruce Yost

Author Affiliations +

Proceedings Volume 7929, Microfluidics, BioMEMS, and Medical Microsystems IX; 79290T (2011) https://doi.org/10.1117/12.881082
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

We designed, built, tested, space-qualified, launched, and collected telemetered data from low Earth orbit from Pharma- Sat, a 5.1-kg free flying "nanosatellite" that supported microbial growth in 48 microfluidic wells, dosed microbes with multiple concentrations of a pharmaceutical agent, and monitored microbial growth and metabolic activity using a dedicated 3-color optical absorbance system at each microwell. The PharmaSat nanosatellite comprised a structure approximately 10 x 10 x 35 cm, including triple-junction solar cells, bidirectional communications, power-generation and energy- storage system, and a sealed payload 1.2-L containment vessel that housed the biological organisms along with the fluidic, optical, thermal, sensor, and electronic subsystems. Growth curves for S. cerevisiae (Brewer's yeast) were obtained for multiple concentrations of the antifungal drug voriconazole in the microgravity conditions of low Earth orbit. Corresponding terrestrial control experiments were conducted for comparison.

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Antonio J. Ricco, Macarena Parra, David Niesel, Matthew Piccini, Diana Ly, Michael McGinnis, Andrzej Kudlicki, John W. Hines, Linda Timucin, Chris Beasley, Robert Ricks, Michael McIntyre, Charlie Friedericks, Michael Henschke, Ricky Leung, Millan Diaz-Aguado, Christopher Kitts, Ignacio Mas, Mike Rasay, Elwood Agasid, Ed Luzzi, Karolyn Ronzano, David Squires, and Bruce Yost "PharmaSat: drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite", Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 79290T (14 February 2011); https://doi.org/10.1117/12.881082

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