A digital rat atlas of sectional anatomy

Li Yu; Qian Liu; Xueling Bai; Yinping Liao; Qingming Luo; Hui Gong

doi:10.1117/12.710690

27 October 2006 A digital rat atlas of sectional anatomy

Li Yu, Qian Liu, Xueling Bai, Yinping Liao, Qingming Luo, Hui Gong

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60470Y (2006) https://doi.org/10.1117/12.710690
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

This paper describes a digital rat alias of sectional anatomy made by milling. Two healthy Sprague-Dawley (SD) rat weighing 160-180 g were used for the generation of this atlas. The rats were depilated completely, then euthanized by Co₂. One was via vascular perfusion, the other was directly frozen at -85 °C over 24 hour. After that, the frozen specimens were transferred into iron molds for embedding. A 3% gelatin solution colored blue was used to fill the molds and then frozen at -85 °C for one or two days. The frozen specimen-blocks were subsequently sectioned on the cryosection-milling machine in a plane oriented approximately transverse to the long axis of the body. The surface of specimen-blocks were imaged by a scanner and digitalized into 4,600 x2,580 x 24 bit array through a computer. Finally 9,475 sectional images (arterial vessel were not perfused) and 1,646 sectional images (arterial vessel were perfused) were captured, which made the volume of the digital atlas up to 369.35 Gbyte. This digital rat atlas is aimed at the whole rat and the rat arterial vessels are also presented. We have reconstructed this atlas. The information from the two-dimensional (2-D) images of serial sections and three-dimensional (3-D) surface model all shows that the digital rat atlas we constructed is high quality. This work lays the foundation for a deeper study of digital rat.

Citation Download Citation

Li Yu, Qian Liu, Xueling Bai, Yinping Liao, Qingming Luo, and Hui Gong "A digital rat atlas of sectional anatomy", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60470Y (27 October 2006); https://doi.org/10.1117/12.710690

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KEYWORDS

3D modeling

3D image processing

Image processing

Brain

Scanners

Tissues

Computed tomography

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