Dynamic saturation optical microscopy

Jan Sýkora; Thomas Dertinger; Jörg Enderlein

doi:10.1117/12.647987

27 February 2006 Dynamic optical saturation microscopy

Jan Sýkora, Thomas Dertinger, Jörg Enderlein

Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920E (2006) https://doi.org/10.1117/12.647987
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A new concept of fluorescence microscopy is presented allowing the breaking of the diffraction limit of optical microscopy by a factor of ca. five. It relies on measuring the temporal evolution of fluorescence after sudden switch-on of the light excitation. The observed temporal dynamics of the fluorescence signal can be converted into information about the spatial distribution of fluorophores within the exciting laser focus. The proposed scheme is technically simple and versatile, and allows resolution enhancement in all three dimensions.

Citation Download Citation

Jan Sýkora, Thomas Dertinger, and Jörg Enderlein "Dynamic optical saturation microscopy", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920E (27 February 2006); https://doi.org/10.1117/12.647987

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