Luminescent probe in the study of surfactant-induced structural changes in serum albumin in human blood plasma

A. G. Melnikov; A. B. Pravdin; V. I. Kochubey; G. V. Melnikov

doi:10.1117/12.634650

22 June 2005 Luminescent probe in the study of surfactant-induced structural changes in serum albumin in human blood plasma

A. G. Melnikov, A. B. Pravdin, V. I. Kochubey, G. V. Melnikov

Author Affiliations +

Proceedings Volume 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI; (2005) https://doi.org/10.1117/12.634650
Event: Saratov, Russia, 2004, Saratov, Russian Federation

Abstract

The luminescence-kinetic technique of the monitoring of structural changes in albumins of human blood plasma that uses a luminescent probe-eosin is proposed. Phosphorescence of eosin bound to the globular proteins of blood plasma-albumins was recorded at room temperature. It is found that under the action of sodium dodecylsulfate on the albumins the rate constant of eosin phosphorescence decay grows and the intensity of eosin phosphorescence decreases. It is assumed that these changes are connected with the denaturing of blood plasma albumins by sodium dodecylsulfate.

Citation Download Citation

A. G. Melnikov, A. B. Pravdin, V. I. Kochubey, and G. V. Melnikov "Luminescent probe in the study of surfactant-induced structural changes in serum albumin in human blood plasma", Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005); https://doi.org/10.1117/12.634650

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