Multimodality of phase and fluorescence in digital holography

Osamu Matoba; Xiangyu Quan; Yasuhiro Awatsuji

doi:10.1117/12.2300989

5 March 2018 Multimodality of phase and fluorescence in digital holography

Osamu Matoba, Xiangyu Quan, Yasuhiro Awatsuji

Proceedings Volume 10714, Third International Conference on Photonics Solutions (ICPS2017); 107140X (2018) https://doi.org/10.1117/12.2300989
Event: Third International Conference on Photonic Solutions, 2017, Pattaya, Thailand

Abstract

Multimodal imaging based on digital holography is presented. There are two types of optical microscopes combined together. One is the digital holographic microscope for phase imaging and the other is epifluorescence microscope for fluorescence imaging. The integration of two optical microscopes is done by choosing the appropriate wavelengths and polarization states of light waves. Preliminary experimental results for biological tissue are presented. To realize 3D fluorescence imaging, incoherent digital holography is required. We present a promising technique based on the selfinterference using diffractive optical elements.

Citation Download Citation

Osamu Matoba, Xiangyu Quan, and Yasuhiro Awatsuji "Multimodality of phase and fluorescence in digital holography", Proc. SPIE 10714, Third International Conference on Photonics Solutions (ICPS2017), 107140X (5 March 2018); https://doi.org/10.1117/12.2300989

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available