Spatiotemporal polarization modulation microscopy with a microretarder array

Changqin Ding; James R. W. Ulcickas; Garth J. Simpson

doi:10.1117/12.2291017

23 February 2018 Spatiotemporal polarization modulation microscopy with a microretarder array

Changqin Ding, James R. W. Ulcickas, Garth J. Simpson

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 1049916 (2018) https://doi.org/10.1117/12.2291017
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

A patterned microretarder array positioned in the rear conjugate plane of a microscope enables rapid polarizationdependent nonlinear optical microscopy. The pattern introduced to the array results in periodic modulation of the polarization-state of the incident light as a function of position within the field of view with no moving parts or active control. Introduction of a single stationary optical element and a fixed polarizer into the beam of a nonlinear optical microscope enabled nonlinear optical tensor recovery, which informs on local structure and orientation. Excellent agreement was observed between the measured and predicted second harmonic generation (SHG) of z-cut quartz, selected as a test system with well-established nonlinear optical properties. Subsequent studies of spatially varying samples further support the general applicability of this relatively simple strategy for detailed polarization analysis in both conventional and nonlinear optical imaging of structurally diverse samples.

Conference Presentation

Citation Download Citation

Changqin Ding, James R. W. Ulcickas, and Garth J. Simpson "Spatiotemporal polarization modulation microscopy with a microretarder array", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049916 (23 February 2018); https://doi.org/10.1117/12.2291017

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