A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging

Xiaodong Tao; Ju Lu; Tuwin Lam; Ramiro Rodriguez; Yi Zuo; Joel Kubby

doi:10.1117/12.2253922

8 February 2017 A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging

Xiaodong Tao, Ju Lu, Tuwin Lam, Ramiro Rodriguez, Yi Zuo, Joel Kubby

Author Affiliations +

Proceedings Volume 10051, Neural Imaging and Sensing; 100510R (2017) https://doi.org/10.1117/12.2253922
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We developed a three-photon adaptive optics add-on to a commercial two-photon laser scanning microscope. We demonstrated its capability for structural and functional imaging of neurons labeled with genetically encoded red fluorescent proteins or calcium indicators deep in the living mouse brain with cellular and subcellular resolution.

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Xiaodong Tao, Ju Lu, Tuwin Lam, Ramiro Rodriguez, Yi Zuo, and Joel Kubby "A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging", Proc. SPIE 10051, Neural Imaging and Sensing, 100510R (8 February 2017); https://doi.org/10.1117/12.2253922

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