Monte Carlo simulations of triplet-state photophysics for super-resolution imaging of fluorophore-labeled gold nanorods

Eric J. Titus; Karole L. Blythe; Katherine A. Willets

doi:10.1117/12.2189926

26 August 2015 Monte Carlo simulations of triplet-state photophysics for super-resolution imaging of fluorophore-labeled gold nanorods

Eric J. Titus, Karole L. Blythe, Katherine A. Willets

Author Affiliations +

Proceedings Volume 9554, Nanoimaging and Nanospectroscopy III; 955403 (2015) https://doi.org/10.1117/12.2189926
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Super-resolution imaging has previously been used to identify the position of individual fluorescently-labeled DNA molecules bound to the surface of gold nanorods. In order to isolate and fit emission from individual fluorophores, a stochastic photoswitching technique based on shelving the fluorophores into triplet states is used. However, the reconstructed super-resolution images of the fluorescently-labeled gold nanorods are consistently smaller than the expected size of the gold nanorod supports. Here, Monte Carlo simulations are used to probe how smaller-than-expected reconstructed images may be obtained by simultaneous emission events based on short triplet state lifetimes and/or a high density of fluorescently-labeled DNA on the gold nanorod surface.

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Eric J. Titus, Karole L. Blythe, and Katherine A. Willets "Monte Carlo simulations of triplet-state photophysics for super-resolution imaging of fluorophore-labeled gold nanorods", Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 955403 (26 August 2015); https://doi.org/10.1117/12.2189926

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