Nonnegative matrix factorization: a blind sources separation method to extract content of fluorophores mixture media

Kenneth J. Zhou; Jun Chen

doi:10.1117/12.2035536

4 March 2014 Nonnegative matrix factorization: a blind sources separation method to extract content of fluorophores mixture media

Kenneth J. Zhou, Jun Chen

Author Affiliations +

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89471V (2014) https://doi.org/10.1117/12.2035536
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The fluorophores of malignant human breast cells change their compositions that may be exposed in the fluorescence spectroscopy and blind source separation method. The content of the fluorophores mixture media such as tryptophan, collagen, elastin, NADH, and flavin were varied according to the cancer development. The native fluorescence spectra of these key fluorophores mixture media excited by the selective excitation wavelengths of 300 nm and 340 nm were analyzed using a blind source separation method: Nonnegative Matrix Factorization (NMF). The results show that the contribution from tryptophan, NADH and flavin to the fluorescence spectra of the mixture media is proportional to the content of each fluorophore. These data present a possibility that native fluorescence spectra decomposed by NMF can be used as potential native biomarkers for cancer detection evaluation of the cancer.

Citation Download Citation

Kenneth J. Zhou and Jun Chen "Nonnegative matrix factorization: a blind sources separation method to extract content of fluorophores mixture media", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471V (4 March 2014); https://doi.org/10.1117/12.2035536

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