Time-resolved fluorescence anisotropy studies of mixed lipid bilayers

A. S. Holmes; J. Cooper; David J. S. Birch; Robert E. Imhof

doi:10.1117/12.17745

1 May 1990 Time-resolved fluorescence anisotropy studies of mixed lipid bilayers

A. S. Holmes, J. Cooper, David J. S. Birch, Robert E. Imhof

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17745
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Time-resolved fluorescence anisotropy of diphenylhexatriene (DPH) in various binary lipid mixtures of dimysterolphosphatidycholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), distearoylphosphatidylcholine (DSPC) and dioleoylphosphatidylcholine (DOPC) has been studied. Evidence is given for phase separation in binary mixtures of DMPC/DSPC and DOPC/DSPC which is not found in DMPC/DPPC and DPPC/DSPC mixtures. A rising anisotropy decay has been observed for DPH in binary lipid mixtures containing DSPC and in DSPC liposomes at the phase transition which suggests a heterogeneous environment for the fluorophore at this temperature.

Citation Download Citation

A. S. Holmes, J. Cooper, David J. S. Birch, and Robert E. Imhof "Time-resolved fluorescence anisotropy studies of mixed lipid bilayers", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17745

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