Characterization of rat liver mitochondria using time-resolved spectroscopy

Hsien-Chang Lee; NaiGuang Wang; Britton Chance

doi:10.1117/12.154642

14 September 1993 Characterization of rat liver mitochondria using time-resolved spectroscopy

Hsien-Chang Lee, NaiGuang Wang, Britton Chance

Proceedings Volume 1888, Photon Migration and Imaging in Random Media and Tissues; (1993) https://doi.org/10.1117/12.154642
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In this study, the (mu) _s' (scattering constant) and the (mu) _a (absorption constant) of rat liver mitochondria were measured by time resolved spectroscopy (TRS) with a newly innovated medium matching method. Freshly isolated rat liver mitochondria were placed inside a pouch and submerged in an intralipid emulsion. Two sets of various concentrations of intralipid emulsion, one with different concentrations of intralipid and no ink and another with various concentrations of ink and a fixed intralipid concentration, were scanned for matching the (mu) _s' and the (mu) _a of the mitochondria. Two states of the mitochondria were scanned: the resting state (orthodox state, or state 4) and the active state (condensed state, or state 3). During the active state of the mitochondria, the active respiration of the mitochondria was initiated by the addition of either calcium chloride or ADP/succinate/P_i. Comparisons of the (mu) _s' and the (mu) _a were made of mitochondria at different states.

Citation Download Citation

Hsien-Chang Lee, NaiGuang Wang, and Britton Chance "Characterization of rat liver mitochondria using time-resolved spectroscopy", Proc. SPIE 1888, Photon Migration and Imaging in Random Media and Tissues, (14 September 1993); https://doi.org/10.1117/12.154642

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