Polarized light scattering as a means of detecting heat and UV-induced changes in bacteria and bacterial spores

Willem Pieter Van De Merwe; Burt V. Bronk

doi:10.1117/12.148345

21 July 1993 Polarized light scattering as a means of detecting heat and UV-induced changes in bacteria and bacterial spores

Willem Pieter Van De Merwe, Burt V. Bronk

Author Affiliations +

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148345
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In the present paper we report on initial attempts to correlate changes in polarized light scattering signals from bacteria and bacterial spores with changes in physical parameters as a result of heat and UV exposure. Clear reproducible changes can be observed, but quantitative correlation such as measuring the degree of germination or survivability needs further study. Average cell size changes can be measured quickly in bacteria such as after UV exposure or during the growth cycle, making the used technique very valuable to monitor changes. Computer modelling is needed to distinguish effects caused by size changes and by changes in index of refraction.

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Willem Pieter Van De Merwe and Burt V. Bronk "Polarized light scattering as a means of detecting heat and UV-induced changes in bacteria and bacterial spores", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148345

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