Analysis of relevant parameters for adapting total internal reflection fluorescence (TIRF) immunosensors to seawater monitoring

Michele S. Celebre; Kayo Umezawa; Maurilio Carra; Claudio Domenici; Danilo De Rossi

doi:10.1117/12.143516

30 April 1993 Analysis of relevant parameters for adapting total internal reflection fluorescence (TIRF) immunosensors to seawater monitoring

Michele S. Celebre, Kayo Umezawa, Maurilio Carra, Claudio Domenici, Danilo De Rossi

Author Affiliations +

Proceedings Volume 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV; (1993) https://doi.org/10.1117/12.143516
Event: Fibers '92, 1992, Boston, MA, United States

Abstract

The conception and implementation of biosensors for monitoring of sea and river water quality are nowadays important topics in environmental research, and the monitoring of water for the presence of toxic substances, even in trace quantities, is foreseen to reach widespread use in the 90s. Because of their high sensitivity and ability to recognize a wide range of substances, immunosensors are particularly suited to this type of measurement. Optical immunosensors based on the total internal reflection fluorescence (TIRF) technique couple the selectivity of the antigen-antibody reaction to the spatial selectivity of the evanescent wave at a refractive boundary. The configuration for the TIRF sensor we have developed is based on a quartz optical fiber with a high numerical aperture.

Citation Download Citation

Michele S. Celebre, Kayo Umezawa, Maurilio Carra, Claudio Domenici, and Danilo De Rossi "Analysis of relevant parameters for adapting total internal reflection fluorescence (TIRF) immunosensors to seawater monitoring", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); https://doi.org/10.1117/12.143516

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