Fabrication of a hybrid plastic-silicon microfluidic device for high-throughput genotyping

Isabelle Chartier; J. Sudor; Yves Fouillet; N. Sarrut; C. Bory; A. Gruss

doi:10.1117/12.478146

17 January 2003 Fabrication of a hybrid plastic-silicon microfluidic device for high-throughput genotyping

Isabelle Chartier, J. Sudor, Yves Fouillet, N. Sarrut, C. Bory, A. Gruss

Proceedings Volume 4982, Microfluidics, BioMEMS, and Medical Microsystems; (2003) https://doi.org/10.1117/12.478146
Event: Micromachining and Microfabrication, 2003, San Jose, CA, United States

Abstract

The lab-on-a-chip approach has been increasingly present in biological research over the last ten years, high-throughput analyses being one of the promising utilization. The work presented here has consisted in developing an automated genotyping system based on a continuous flow analysis which integrates all the steps of the genotyping process (PCR, purification and sequencing). The genotyping device consists of a disposable hybrid silicon-plastic microfluidic chip, equipped with a permanent external, heating/cooling system, syringe-pumps based injection systems and on-line fluorescence detection. High throughput is obtained by performing the reaction in a continuous flow (1 reaction every 6min per channel) and in parallel (48 channels). We are presenting here the technical solutions developed to fabricate the hybrid silicon-plastic microfluidic device. It includes a polycarbonate substrate having 48 parallel grooves sealed by film lamination techniques to create the channels. Two different solutions for the sealing of the channels are compared in relation to their biocompatibility, fluidic behavior and fabrication process yield. Surface roughness of the surface of the channels is the key point of this step. Silicon fluidic chips are used for thermo-cycled reactions. A specific bonding technique has been developed to bond silicon chips onto the plastic part which ensures alignment and hermetic fluidic connexion. Surface coatings are studied to enhance the PCR biocompatibility and fluidic behavior of the two-phase liquid flow. We then demonstrate continuous operation over more than 20 hours of the component and validate PCR protocol on microliter samples in a continuous flow reaction.

Citation Download Citation

Isabelle Chartier, J. Sudor, Yves Fouillet, N. Sarrut, C. Bory, and A. Gruss "Fabrication of a hybrid plastic-silicon microfluidic device for high-throughput genotyping", Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); https://doi.org/10.1117/12.478146

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