Abstracts

s 3 manipulation of tragedy’s articulation of socio-political and ideological problems may in turn illuminate the Aeneid as a national epic and its much-contested relationship with Augustan ideology. More speci cally, I argue that issues of identity and moral action explored in Sophocles’ Ajax are crucial in the cases of Dido and Turnus, who similarly  nd themselves in con ict with and unable to adapt to the new social and political structure of Aeneas’ new order. Like Ajax, Dido and Turnus de ne themselves through constant reference to their relationship with their people. All three, however, engage in action which pits them against the interests of their communities and which results in their complete isolation. Unable to adjust their behavioral code to ensure their survival, Dido and Turnus embody a heroic ideal which, though laudable, can have no place in Aeneas’ Roman future. Vergil thus mobilizes a tragic allusive register in order to illustrate the tension between the celebration of this ideal and the realization that social change has rendered it obsolete. At the same time, the loss of the ideals that Dido and Turnus represent necessitates the articulation of a comparable, if not superior, ethical code which Aeneas is called on to embody. Allusive evidence linking Aeneas to the tragic Ajax, however, seems to indicate Aeneas’ failure to emerge as a superior moral force in the poem. Anastasia-Erasmia Peponi, “Mixed Pleasures, Blended Discourses: Poetry, Medicine, and the Body in Plato’s Philebus 46-47c” In Plato’s Philebus the last section of the discussion on the falseness of pleasure is dedicated to those pleasures intrinsically mixed with pain. This paper focuses speci cally on bodily mixed pleasures, an analysis that extends from 44d to 47c, while its focal point is 46-47c. By adopting the anti-hedonists’ methodology, Socrates cunningly transforms his entire analysis of bodily mixed pleasures into a discourse on human disease, in which medical terminology prevails. Two major points are made in the reading suggested here. (a) Despite Socrates’ quasi-medical language, a substratum of poetic discourse is underlying his analysis. Thus, a network of poetic associations—probably promptly recalled by Plato’s audience—not only reveals the intertextual encounter between medical, philosophic and poetic discourses but also contributes to the interpretation of Socrates’ analysis. Hence, the pathology of love as expressed through poetry illuminates the meaning, questionable in straightforward medical terms, of passage 46c6-d2, while it also reveals the unfolding unity underlying Socrates’ analysis, otherwise thought to comprise three distinct medical cases. (b) Ancient medical lore is important for the understanding of passage 46d7-47a1 on the aZiction of knêsis and psôra, not only because it con rms the analogy between the physiology of knêsis and that of sexual arousal and climax, but also because it illuminates the speci c medical treatment Socrates is describing. Thus, in the crucial debate about whether Plato uses the term aporiais or pyriais in 46e2, medical evidence seems to support the latter. The reading pyriais makes this complicated paragraph more comprehensible in terms of meaning and syntax, while stressing the importance of heat in the processes of both the curing of knêsis and of human orgasm. Besides, it further con rms the intertextual encounter between the Phaedrus and the Philebus. D ow naded rom http://online.ucpress.edudf/21/1/1/80926/ca_2002_21_1_1.pdf by gest on 11 June 2020

METHODS: We reviewed 635 Filipino HIV cases from 2004 to 2016, using multivariate regression to determine risk factors for hyperlipidemia and hyperglycemia before and after antiretroviral (ARV) use. RESULTS: In ARV-na€ ıve patients, male sex, hyperglycemia, and ARV delay>320 days from diagnosis were risk factors for hyperlipidemia, while hyperlipidemia was a risk factor for hyperglycemia. After ARV initiation, hyperglycemia, ARV use>36 months, WHO Stage 4 HIV, and an efavirenz-based regimen were risk factors for hyperlipidemia, while age>30, hyperlipidemia, overweight BMI, and a zidovudine-based regimen were risk factors for hyperglycemia. CONCLUSION: Various factors contribute to hyperlipidemia and hyperglycemia among Filipino HIV cases.

AII-5-2
Effectiveness assessment for the treatment and management platform of obesity in Korean child and youth We aim to assess the effectiveness of the improvement in BMI percentile by applying integrated platform developed to treat and manage of childhood obesity in local community. This study was performed over 12-months as a randomized clinical trial. A total of 154 subjects randomly assigned to two groups at baseline. Of those, 71 study subjects followed up were included for analysis. A 2 by 2 mixed model ANOVA was done to compare the change of BMI percentile. There was a significant interaction between group and time (P = 0.045). In intervention group, BMI percentile significantly decreased from 93.1 to 90.9. These findings show that effective management and treatment of childhood obesity will be available by using platform in local community.

AII-5-3
Mobile based nutritional management tool for pregnant women with diabetes mellitus in Korea The aim of this study was to develop the mobile based nutritional management tool can be designed for supporting self-nutritional management for pregnant women with diabetes and evaluate feasibility. Mobile application as management tool of subject's diet has four main functions which are as follows.
(1) Assessment appropriate maternal weight gain for gestational week, (2) Prescription total calories considering pre-pregnancy weight and gestational week. And Suggestion of a recommended diet, (3) Providing nutritional information (4) Expert's evaluation of dietary records, user-entered. This application was meaningful in that it was easy and convenient for using and helpful for self-nutritional management of pregnant women with DM.

AII-5-4 Outcomes after hospitalization of diabetes in older patients received influenza vaccination
We investigated the association between severe hypoglycemia and risk of dementia in person with DM. A total of 67,485 people with DM include in the analysis. During a median of 10.5 years of follow-up (interquartile range, 10.0-11.2), 8,951 people had a first diagnosis of dementia. Those who experienced a hypoglycemic event had a 2-fold increased risk for developing dementia compared with those who did not have a hypoglycemic event (OR, 1.88; 95% CI, 1.65-After adjusted confounding variables, the association between severe hypoglycemia and risk for dementia remained significant (OR, 1.18; 95% CI, 1.02-1.36). Our results provide evidence for an association between severe hypoglycemic events and dementia in person with diabetes.
Glycemic variability (GV) affects the outcome of critically ill patients. To determine the effect of GV on inpatient mortality in the elderly, we did a retrospective cohort study with patients ≥65y/o admitted to ICU. GV indices were higher among those who died but there was no significant association. There was a trend towards greater inpatient mortality with increasing GV with mean glu-cose>180 mg/dL. Hence, GV is not a significant prognostic factor in critically elderly patients.

AII-6-7
Association study of 14 single nucleotide polymorphisms and insulin resistance in a Chinese population We found that the expression of Follistatin like-3 (FSTL3) in subcutaneous and visceral fat was increased at the very early stage of obesity and correlated with BMI.
The expression of FSTL3 was upregulated in fat samples of db/db mice. Overexpressing of FSTL3 exhibited impaired glucose metabolism and downregulation of FSTL3 improved insulin sensitivity.
FSTL3 is known to bind to Activin, and we found that expression level of G6Pase and PEPCK, key regulators of gluconeogenesis, was suppressed by treatment with recombinant Activin in primary hepatocyte.
We found that the effect of impaired glucose metabolism of FSTL3 appears to be though inhibition of Activin which exhibited lower glucose levels by suppression of gluconeogenesis.

AII-6-9
Serum aryl-hydrocarbon receptor transactivation assay reveals persistent nature of diabetogenic chemicals Serum AhR transactivation activity and mitochondrial inhibitor activity are predictive of future development of T2D among Koreans, suggesting that persistent organic pollutants is involved in the pathogenesis. Further analysis of Korean Genome Epidemiology study samples showed smokers had higher AHRTA (RR 12.14, 95%CI 2.766-53.13, P < 0.001) and ex-smoker still had higher AHRTA (RR 3.2, 95%CI, 1.14-8.99, P < 0.027) than non-smokers. Serum AHRTA and MIA reflected previous diabetic state; those subjects who had diabetes in 2002 had higher AHRTA and MIA and more diabetes had developed during 2002-2008 period among the subjects belonging to higher AHRTA and MIA quartiles. These observations suggest diabetogenic chemicals persist in human body.

Department of Medicine and Molecular Science
Metabolic stress is associated with dysfunctions of AMP-dependent protein kinase (AMPK). Additionally pro-inflammatory cytokines such as TNFa contributes to insulin resistance. Previously we reported that Fyn regulates AMPK activity by direct phosphorylation of Y426 of AMPKa. TNFa increased Fyn kinase activity and siRNA knockdown of Fyn prevented TNFa inhibition of AICAR stimulated AMPK activation. TNFa induced inhibition of autophagy was not observed when AMPKa was mutated on Y436. These data demonstrate that Fyn plays an important role of TNFa on autophagy via phosphorylation of AMPK.

AII-6-11
Enhanced expression of Survivin plays distinct roles in adipocyte homeostasis Survivin is a gene that has been found to abundantly expressed in fetal tissues and the most common human tumors, and was considered absent in normal tissues in initial studies.
Here, we report that Survivin can be expressed upon insulin stimulation in vitro and under high fat diet feeding conditions in vivo. The ectopic expression of Survivin in 3T3-L1 cells does not affect adipocyte differentiation, but rather inhibits isoproterenol-stimulated adipocyte lipolysis via the b-Adrenergic/ cAMP pathway. Survivin also attenuates DNA damage-related stress responses, as well as TNFa-induced lipolysis. Taken together, the current findings suggest that Survivin may facilitate adipocyte maintenance in response to obesity-induced adipocyte stress. AII-6-12 SFPQ, a pre-mRNA splicing factor interacts with phosphorylated HELZ2 and plays a novel role in adipocyte differentiation A. T. Katano 1 , T. Satoh 1 , T. Tomaru 1 , S. Yoshino 1 , T. Takamizawa 1 , T. Watanabe 1 , T. Okamura 1 , S. Matsumoto 1 , K. Horiguchi 1 , Y. Nakajima 1 , S. Understanding of molecular mechanism for adipocyte differentiation would contribute to the management of glucose intolerance, an obesity-related disease. We previously identified HELZ2, a coregulator of PPARc. To further elucidate the pathological process of obesity, we here isolated splicing factor proline/glutaminerich (SFPQ), a pre-mRNA splicing factor as a protein that interacts specifically with tyrosine-phosphorylated HELZ2. Knockdown of Sfpq in 3T3-L1 cells resulted in reduced fat accumulation due to aberrant expression of transcription factors that play essential roles in the early stage of adipocyte differentiation and deranged pre-mRNA alternative splicing. SFPQ may be a novel target for the treatment of obesity-related disease.

AII-6-13
Imeglimin increases insulin secretion in response to glucose as a unique mechanism of action depending on NAD synthesis S. Hallakou-Bozec 1 , S. Bolze 1 , M. Kergoat 2 , P. Fouqueray 1 and K. Takeda 1 1 Poxel, 2 M etabrain Research Imeglimin (IME) is a novel glucose-lowering agent improving insulin secretion in response to glucose (GSIS) and insulin sensitivity. IME is currently in phase 2b in Japan. IME increases NAD content involving the NAD synthesis salvage pathway. Its GSIS effect does not involve classical insulin secretion effectors and is synergic with GLP-1. In T2D patients IME increases insulin secretion. IME increases b-cell mass, decreases the number of apoptotic b-cells and increases the proportion of proliferative b-cells.
These results demonstrate that IME-induced GSIS involves the NAD synthesis salvage pathway and may protect b-cell mass.

AII-6-14
Effect of liraglutide and monoclonal FGFR2-IIIc antibody on the development of obesity and type 2 diabetes in mice K. Nonogaki Department of Diabetes Technology, Tohoku University Graduate School of Biomedical Engineering We examined the effect of liraglutide, a glucagon-likepeptide-1 (GLP-1) receptor agonist, and monoclonal fibroblast growth factor receptor 2c (FGFR2c) antibody on the development of obesity and type 2 diabetes in mice. Systemic administration of liraglutide significantly decreased food intake, body weight, blood glucose levels and the expression of FGFR2 in epididymal white adipose tissue at 24 h after its administration while having no significant effects on plasma insulin and glucagon levels in individually housed KKA y mice. Treatment with FGFR2-IIIc antibody significantly suppressed body weight gain and epididymal white adipose tissue weight by increased lipolysis without affecting food intake and blood glucose levels in KKA y mice.

AII-6-15
A limonene-derivative purified from Sudachi peel upregulates sirt1 and improves lipid/glucose metabolism in HFD-fed mice Citrus Sudachi is a small sour typical seasoning in Japanese cuisine. A daily administration of Sudachi peel extended lifespan of ZDF rats. Here we determined active compounds of the sudachi peel. We successfully identified a limonene-derivative as an active molecule by an index of TG-reducing effects in cultured cells. It increased sirt1 expression levels, and the TG-lowering effects was sensitive to nicotinamide, suggesting involvement of sirt1. In HFD-fed mice, the compound improved glucose tolerance, fatty liver, serum TG and cholesterol levels with increase in sirt1 activities. Thus, it should be one of the active components of Sudachi peel regulating metabolism, which is supposed to be mediated by sirt1 upregulation. Haptoglobin (Hp) is an acute-phase protein that is crucial for the neutralization of oxidative damage. We aimed to investigate the relationship between polymorphisms in Hp (Hp1-1, Hp2-1 and Hp2-2) and macroangiopathies in Chinese patients with type 2 diabetes (n = 6355). Hp genotyping was performed using TaqMan-based real-time PCR. Results showed that Hp 2 allele was negatively correlated with carotid diameter (P = 0.0242), carotid intima-media thickness (P = 0.0312) and Hp serum levels (P < 0.0001). After adjusting for variables, we found a significant association with diabetic macroangiopathies (OR = 0.859, 95%CI 0.745-0.990, P = 0.0354). Our data indicate that Hp polymorphisms were associated with diabetic macroangiopathies in a Chinese population.

AI-P-2
GCK-MODY is not rare in a Chinese community population Y. Ma 1 , X. Han 2 , L. Ji 2 , X. Zhou 2 and Y. Li 1 1 Beijing Pinggu Hospital, 2 Peking University People's Hospital The aim of our study is to estimate the prevalence of MODY2 in a Chinese community population. In a Chinese community population including 3345 subjects in Pinggu district, Beijing, China, 252 of 336 patients newly diagnosed with T2DM were screened for mutations in exons of GCK. Three known causative mutations (p.R43H, p.G44S, p.R377L) and two novel deleterious mutations (p.T82P, p.A259S) were identified in five patients, which didn't exist in 300 normal subjects. The proximate prevalence of MODY2 in the Chinese population ranged from 0.09% to 0.15%. Our study indicates MODY2 is not rare in Chinese population.

AI-P-3
Predicting effects of G6PC2 on type 2 diabetes and impaired glucose regulation in a 9-year prospective Chinese cohort The aim of this study was to investigate the predicting effects of common variants of G6PC2 on deterioration of blood glucose and change in glucose metabolism traits in in a community-based 9-year prospective cohort (n = 2,547) of Chinese population. Three SNPs (rs492594, rs2232328 and rs16856187) of G6PC2 were genotyped. Only rs16856187 has predicting effects on incidence of T2D and IGR with trend (P = 0.074) after adjusting for age, sex, BMI at baseline in logistic regression. Moreover, none of the three SNPs show predicting effects on changes in glucose metabolism traits. In this study we did not identify the predicting effects of G6PC2 variants on blood glucose deterioration and change in glucose metabolism traits.

AI-P-4
The association of variations in COX1 and COX2 with type 2 diabetes and glucose metabolism in Chinese Han populations B. Xu 1,2,3 , C. Hu 1,2,3 and W. Jia 1,2,3 1 Department of Endocrinology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 2 Shanghai Diabetes Institute, 3 Shanghai Key Laboratory of Diabetes Mellitus AIM: Cyclooxygenases (COX) are vital enzymes in inflammation, proved to be associated with type 2 diabetes (T2D). We aimed to test whether polymorphisms in COX1 and COX2 are associated with T2D and glucose metabolism in Chinese. METHODS: We included 6822 individuals with OGTT data and genotyped tag SNPs of COX1 and COX2. RESULTS: rs3218625 of COX2 was correlated with T2D after adjusting for age, gender and BMI (OR = 0.74, 95% CI = 0.56 to 0.97, P = 0.031). Moreover, variants in both COX1 and COX2 displayed association with glucose related traits, including glucose level, insulin secretion and sensitivity (P = 0.002-0.049). CONCLUSION: Our study suggests that COX might play a part in the prevalence of T2D and glycometabolism in Chinese Han populations.

AI-P-5
Variations in KCNJ11 are associated with early-onset type 2 diabetes mellitus in Chinese population OBJECTIVES: To investigate if the E23K (G>A) or A190A (C>T) in KCNJ11 are associated with early-onset T2DM in Chinese. METHODS: A case-control study was conducted in 175 early-onset T2DM patients who receive insulin (ins+, n = 57) or not (ins-, n = 118), and 182 control subjects, who were genotyped by PCR-sequencing. RESULTS: Higher E23K-GA+AA and lower A190A-TT in T2DM, especially in the ins+ group; higher 2 h plasma glucose and lower 2 h insulin in E23K-AA (vs. E23K-GG) of control group were observed (all P < 0.05). A190A-TT had higher SBP than CC In ins+ group (P < 0.05). CONCLUSIONS: The E23K may increase the risk of early-onset T2DM which can be protected by A190A-TT. The A190A-TT or E23K-GG may elevate the risk of hypertension in the Chinese.

AI-P-6
Association of PAX4 polymorphism with diabetic retinopathy in Chinese population Y. Li, C. Hu and W. Jia Department of Endocrine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital OBJECTIVES: This study aims to investigate the association between rs10229583 near PAX4 and diabetic retinopathy (DR) susceptibility in a Chinese T2DM cohort. METHODS: We genotyped rs10229583 in T2D patients with DR, and without DR whose duration is over 5 years (n = 998 and 1,373, respectively). All patients underwent full ophthalmological examination. RESULTS: No evident significant association was found between DR and rs10229583 (P = 0.8031, OR = 1.021) in logistic analysis. Similar result was found (P = 0.7831, OR = 0.976) after adjusting for the diabetic duration, HbA1c, blood pressure and BMI. CONCLUSIONS: Rs100229583 near PAX4 had no significant effects on DR in Chinese population.

AI-P-7
Baseline level and change in serum albumin concentration and the risk of incident type 2 diabetes Baseline serum albumin concentration was associated with an unfavorable metabolic phenotype and partly with future risk of type 2 diabetes. However, change in serum albumin could better explain the association between serum albumin concentration and diabetes risk than did a single measurement; that is, an increase in serum albumin concentration was inversely and independently associated with a lower risk for incident T2D.

AI-P-8
Elevation in serum fibroblast growth factor 23 levels in normoglycemic first-degree relatives of patients with diabetes The goal of this study was to explore alteration in serum fibroblast growth factor 23 (FGF23) levels and its value for identifying subclinical atherosclerosis in normoglycemic subjects with a first-degree family history of diabetes (FHD). Serum FGF23 levels were higher in 312 subjects with a first-degree FHD than in 1407 subjects without a FHD. A first-degree FHD was positively and independently associated with serum FGF23 levels. In subjects with a first-degree FHD, only those with serum FGF23 levels in the upper quartile were more likely to have an increased C-IMT. As conclusion, the influence of a first-degree FHD on serum FGF23 levels should be considered to avoid overestimating the cardiovascular disease risk.

AI-P-9
Heavy alcohol drinking as a nonglycemic parameter to determine hemoglobin A1c in a Korean population Among the 24,594 participants who participated in the 2011-2013 Korea National Health and Nutrition Examination Survey (KNHANES), 12,923 participants were analyzed in this study. Logistic regression analyses showed that, when using the group that abstained as the control, the group that consumed alcohol ≥ 30 g/day was negatively associated with the risk of an HbA1c level of ≥5.7%, ≥6.1%, and ≥6.5% even after adjusting for confounding factors, including the FPG concentration, in this nationally representative sample of Korean adults. These results suggest that excessive drinking shifts the HbA1c level downward, which might complicate use of the HbA1c level for the diagnosis of diabetes or prediabetes.

AI-P-10
Comparisons between early onset and late onset patients of the glucose control in newly diagnosed T2DM in China X. Cai, X. Gao, X. Han and L. Ji Peking University People's Hospital To learn the baseline characteristics and the glucose control in early onset newly diagnosed type 2 diabetes patients, we performed an observational and prospective study in a cohort of newly diagnosed T2DM patients in China. A total of 5770 patients from 79 hospitals were included. The early onset patients were defined as patients with the diagnosed age no more than 40 years old. Totally 666 patients were early onset diabetes patients. Comparisons between early onset and late onset patients of the baseline characteristics indicated that in China, in newly diagnosed patients, early onset patients tended to be more males, more smokers, more obese, higher baseline HbA1c level, but decreased more after one year of treatment.

AI-P-12
The association of CYP2C9 and CYP2J2 variants with type 2 diabetes risk in Chinese populations CYP450-mediated epoxyeicosatrienoic acids exert favorable effects on glucose homeostasis. We aim to test the impacts of CYP2C9 and CYP2J2 variants on type 2 diabetes (T2D) risk in Chinese populations. We totally genotyped 22 tagging loci of CYP2C9 and CYP2J2 in 6822 participants and collected the clinical measurements. We found that CYP2C9 rs1819173 was associated with T2D (OR = 1.19, 95%CI 1.10-1.28, P = 6.93 9 10 -6 ) and it remained significant even adjusting for multiple testing (empirical P = 0.0003). Moreover, we observed that CYP2C9 rs1934967 and CYP2J2 rs10789082 and rs1593461 were associated with insulin secretion and sensitivity indies (P = 0.02-0.04). It is indicated that CYP2C9 and CYP2J2 may play an important role in glucose metabolism.

AI-P-13
Retrospective review on the outpatients with type 1 diabetes mellitus at Chiba University Hospital As the number of foreign visitors and residents in Japan increase, Japanese medical staff is increasingly likely to encounter foreign patients with diabetes. However, there are several factors complicating diabetes management for these patients. Diet therapy may be especially difficult to implement because eating habits are unique to each culture and ethnicity. Differences in language, cultural habits, healthcare insurance, and medications are other potential barriers. Based on our experiences, we analyzed these problems and concluded that to provide better diabetes care to foreign patients, efforts should be made to understand their cultural backgrounds, food habits, and the diabetes treatment guidelines used in their native countries.

AI-P-19
Usefulness of glucose parameters obtained from Oral glucose tolerance test (OGTT) in Korean subjects We evaluated OGTT results from 494 subjects who had performed an OGTT. We measured the OGTT parameters (G 0 , G 60 , G 120 ), insulin, C-pep, HbA1c during OGTT. OGTT parameters significantly correlated with HbA1c, insulin, and C-peptide in total subjects. OGTT parameters using the possibility of HbA1c ≥6.5% is the highest in G 0 and G 60 is the lowest. Using G 0 , G 60 and G 120 , the highest rate of HbA1c ≥6.5% when G 0 and G 120 were used together, and the lowest when using G 60 and G 120 . However, odds ratio of HbA1c ≥6.5% is the highest when G 60 and G 120 were used combined. From our results of OGTT, the useful result of diabetes diagnosis is G 0 and G 120 , so the measurement and usefulness of G 60 value in OGTT should be reviewed from large studies.

AI-P-20
Serum 1,5-anhydroglucitol levels slightly increase after a glucose load The goal of this study was to explore short-term changes in serum 1,5-anhydroglucitol (1,5-AG) levels after a glucose load. A total of 681 participants without a prior history of diabetes mellitus (DM) were enrolled. Serum 1,5-AG levels were slightly elevated after a glucose load, the difference between peak and baseline levels (D1,5-AG) was significantly lower in DM patients than in individuals without DM. D1,5-AG was positively associated with baseline 1,5-AG was also found. Although serum 1,5-AG levels have been shown to decline with long-term hyperglycemia, the present study showed that serum 1,5-AG levels slightly increased after a glucose load.

AI-P-21
Inverse association between fasting insulin levels and postprandial asprosin level changes in Type 2 diabetes patients Asprosin (ASP) has recently been discovered as a glucogenic hormone secreted from adipose tissue. While ASP promotes hepatic glucose release, the pathophysiological role of ASP in patients with glucose intolerance remains unclear. We aimed to evaluate the relationship between ASP postprandial concentration kinetics and other biomarkers. 8 healthy subjects and 23 type 2 diabetes mellitus (T2DM) underwent a 2-h meal tolerance test. In T2DM patients, postprandial reduction of ASP levels showed a significant negative association with fasting insulin levels (r = -0.41 P < 0.05). Fasting insulin levels were negatively correlated with postprandial ASP level reduction, suggesting that insulin could regulate postprandial ASP levels.

AI-P-22
Response to 75g glucose tolerance tests: comparison of type 2 diabetes and healthy control S. Tserensodnom 1 , B. Byambatsogt 1 and S. Sonomtseren 2 1 Endomed Clinic, 2 Mongolian National University of Medical Science OBJECTIVE: To compare response to 75 g-OGGT in patients with newly diagnosed T2DM and healthy volunteers. METHODS: Each facility was enrolled 15 patients with newly diagnosed T2DM and 15 healthy controls, totally 30. OGGT wasconducted and samples were collected at 0 and 120 min after glucose administration. RESULTS: Response to OGGT was abnormal for all T2DM patients. Among healthy controls IFG were 4 (26.7%), IGT were 3 (20.0%) and abnormal HbA1C were 2 (13.3%). CONCLUSIONS: Responses to 75 g glucose tolerance tests are abnormal for all T2DM patients andtwo third of their have a critical high level of blood glucose in last 3 months. Among healthy controls one third have an IFG and one fifth have an IGT.

AI-P-23
HbA1C and mean glucose derived from continuous glucose monitoring assessment do not correlate in patients with HbA1c >8% Y. Tagaya, E. Yamada, Y. Nakajima, A. Osaki, Y. Shimoda, R. Shibusawa, T. Saito, T. Sato, S. Okada and M. Yamada Department of Medicine and Molecular Science Gunma University Graduate School of Medicine AIMS: Optimum therapy for patients with diabetes depends on both acute and long-term changes in plasma glucose (PG) as assessed by HbA1c. We examined this relationship using continuous glucose monitoring (CGM). METHODS: 51 patients (70% women, 30% male) were examined. HbA1c were compared with PG determined by CGM. RESULTS: Changes of HbA1c up to 8.0% showed a statistically strong relationship (R = 0.6713, P < 0.0001) with mean PG measured by CGM. However, when HbA1c was more than 8.0% there was no statistically significant relationship (R = 0.0498, P = 0.8298). CONCLUSIONS: CGM data is a good clinical indicator of long-term glucose control, however cautions in interpreting CGM data should be taken when the HbA1c are above 8.0%.

AI-P-24
Comparison of markers between metabolically obese but normal weight and metabolically healthy but obese in Korean adults The aim of this study was to compare multiple markers between metabolically obese but normal weight (MONW, n = 412) and metabolically healthy but obese (MHO, n = 518) in total 2,917 adults Vitamin D was significantly decreased in subjects with MONW (15.7 -7.1 ng/mL) compared with MHO (16.9 -6.4 ng/ mL). However, fatty liver index (FLI) and cystatin C were significantly increased in subjects with MHO (FLI, 47.1 -21.1; cystatin C, 0.88 -0.16 mg/L) compared with MONW (FLI, 36.9 -20.8; cystatin C, 0.85 -0.16 mg/L). There were no significant differences in CRP, homocysteine, GGT, and HOMA-IR between two groups. Low vitamin D may be more related with metabolically obese, but high FLI and cystatin C may be more related with obesity itself.

AI-P-25
The association of TCF7L2 genetic variants with diabetic retinopathy in Chinese population X. Wang, R. Zhang, C. Hu and W. Jia Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital TCF7L2 has been confirmed to be associated with T2D and diabetic retinopathy (DR) in Caucasians. However, the effects of TCF7L2 variants on DR in Chinese is still unknown. We genotyped rs7903146 in T2D patients with and without DR (n = 998 and 1,373, respectively). No significant association was observed of rs7903146 and DR after adjusting duration, HbA1c, blood pressure and BMI in logistic analysis (OR = 0.869, P = 0.4011). In conclusion, TCF7L2 rs7903146 had no significant effects on DR in Chinese population.

AI-P-26
One Stop Service for survey of diabetic complications C. S. Chen and P.-S. Cheng Department of Endocriniology and Metabolism, Taipei City Hospital INTRODUCTION: Diabetes mellitus with poor glycemic control was notorious for its chronic complications. METHODS: One visit in our clinics, we will provide survey of urine albumin excretion rate (UAE), non-mydriatic retinal camera,and quantitative sensory testing (QST). Peripheral artery disease was also assessed by ankle brachial index (ABI). We will check the rate before and after the service. RESULTS: The percentage of four examinations were increased. Retinal examination from 67.6% to 72.8%, UAE from 63.5% to 79.8%, QST from 0% to 45%, and ABI from 89.9% to 95.1%. CONCLUSIONS: One stop survey complications of diabetes were a patient-center service. Integrated care of patient could increase the percentage of diagnosed complication.

AI-P-27
Association between FTO gene polymorphisms and osteoporosis in patients with T2DM BACKGROUND: In this study, We investigated the association of FTO polymorphisms with BMD and other parameters in patients with T2DM. METHODS: 120 patients were included in this study. The FTO polymorphisms rs1861868, rs1421085 and rs9939609 were determined. BMD Other clinical parameters were measured. RESULTS: Patients with osteoporosis were more likely to carry the rs9939609 A allele compared to normal patients with BMD. Two haplotypes (ACA and GCA) were associated with higher risk of osteoporosis. Patients without the ACA allele showed lower T-score at the femoral neck and an increased risk of osteoporosis. CONCLUSION: Genetic variations in FTO are associated with osteoporosis among patients with T2DM.

AI-P-28
Risk of diabetes in patients of chronic obstructive pulmonary disease: a retrospective cohort study We evaluated diabetes risk in chronic obstructive pulmonary disease (COPD) patients. We identified 14720 adults with COPD exacerbations (COPDe) and 29440 non-COPD controls in 2000-2008. Diabetes events in 2000-2013 were identified in the follow-up period. Adjusted HR of diabetes associated with COPD were calculated. The incidences of diabetes for patients with no COPD and COPDe were 2.4 and 5.2 per 1,000 person-years, respectively. Patients with COPDe (HR 2.53) had increased risk of diabetes. The association between COPDe and diabetes risk was significant in both sexes and every age group. We concluded that COPDe was associated with diabetes risk.

AI-P-29
Effect of sleep disorders in patients with diabetes mellitus We have attempted to evaluate the characteristics of sleep parameters and sleep quality of patients with diabetes mellitus using questionnaires on individual sleep quality. The participants asked the Pittsburgh Sleep Quality Index (PSQI). We analyzed 100 patients with diabetes mellitus. The average total score of PSQI was 4.39 -0.23. Patients with over 6 scores of PSQI are considered to have sleep disorder, and 32 patients out of 100 patients meet this definition and grouped as high PSQI. 68 patients were classified as low PSQI group. Systolic blood pressure in the group of the high PSQI was significantly higher than in low PSQI group. In addition average BMI of high PSQI was 23 -0, significantly lower than 26 -1 in the low PSQI group.

AI-P-30
Long term risk of stroke in type 2 diabetic patients with diabetic ketoacidosis We extracted claims data for 3572 type 2 diabetic patients with DKA and 7144 controls between 2000 and 2002. All patients were tracked until new stroke diagnosis, death, or end of 2011. Two hundred seventy of the 3572 patients with diabetic ketoacidosis and 404 of 7144 of the controls were diagnosed as having a new stroke, incidence rate ratio (IRR) 1.56 (95% CI 1.34-1.82; P < 0.0001). Patients with diabetic ketoacidosis had a higher risk of ischemic stroke than those without (IRR 1.62, 95% CI 1.34-1.96; P < 0.0001). After adjustment for patient characteristics and comorbidities, diabetic ketoacidosis patients were 1.55 times more likely to have a stroke than those without diabetic ketoacidosis (95% CI 1.332-1.813, P < 0.0001).

Division of Diabetes and Endocrinology, Masuko Memorial Hospital/Nagoya Daini Red Cross Hospital, 2 Division of Diabetes and Endocrinology, Nagoya Daini Red Cross Hospital
Endothelial dysfunction associates with postprandial hyperglycemia. We conducted observational study of concecutive 108 type 2 diabetic patients and examined thereafter for 30 months the composite endpoint, including major adverse cardiovascular event. This cohort patients' age, body mass index, HbA1c, eGFR, LDL cholesterol levels were 62.3 -13.6 years, 26.3 -4.9 kg/m2, 10.3 -2.0%, 69.9 -18.7 mL/min, 113 -36.2 mg/dL, respectively. Flow-mediated dilatation (FMD) and 1-h postprandial glucose levels (1hPPG) had negative correlation. Interestingly, eGFR, 1hPPG and FMD were all associated with the composite endpoint by logistic regression analysis. Postprandial hyperglycemia-mediated endothelial malfunction may predict future adverse events.

AI-P-32
The synergistic impact of apolipoprotein B/A-1 and lipoprotein (a) on coronary artery calcification K. Park, J. Kim, S. Lee, J. You, J. Park, J. Nam, C. Ahn, K. Kim and Y. Kim

Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine
We investigated the influence of Apo B/Apo A-1 and Lp(a) on coronary artery calcification (CAC) among healthy Korean adults. A total of 1081 participants were enrolled. Anthropometric profiles and Apo B, Apo A-1, and Lp(a) were measured. Multi-detector CT was used to measure coronary artery calcium score (CACS) and CACS>0 was defined as the presence of CAC. The prevalence of CAC significantly increased with Lp(a) and Apo B/Apo A-1 levels. In the logistic regression analysis adjusted for multiple risk factors, odds ratio for the prevalence of CAC comparing the lowest Lp(a) and Apo B/Apo A-1 group to the highest group was 2.554 (1.26-5.20) (P < 0.05). These results show that Apo B/Apo A-1 and Lp(a) have a synergistic impact on prevalence of CAC.

AI-P-33
Establishment of a zebrafish system for diabetes complications by novel acceleration plethysmogram and electrocardiogram We identified 49 circulating miRNAs specifically regulated with insulin resistance, and two of which were predicted as miRNAs targeting arteriosclerosis-related genes. So we developed an evaluating zebrafish system for arteriosclerosis and diabetic autonomic neuropathy by the acceleration plethysmogram and nonaggression type electrocardiogram. We demonstrated that high fat diet significantly increased SDPTGAI showing arteriosclerosis, and caused patterns of electrocardiogram indicating coronary ischemia, and furthermore, significantly decreased parasympathetic nerve activity in power spectrum.
Zebrafish was shown to be a useful model to quickly evaluate arteriosclerosis, heart ischemia and early stages of diabetic autonomic neuropathy.

AI-P-34
Fibroblast growth factor 23 strengthen the capacity of Framingham Risk Score to identify subclinical atherosclerosis The goal of this study was to investigate the relationship between serum fibroblast growth factor 23 (FGF23) levels and carotid intima-media thickness (C-IMT) in men with a low-to-moderate cardiovascular disease (CVD) risk assessed by Framingham Risk Score (FRS). This study enrolled 392 and 372 men with a low and moderate CVD risk, respectively. The results showed that men with high serum FGF23 levels, and no matter with a low or moderate CVD risk, were more likely to have an increased C-IMT than those with low serum FGF23 levels and a low CVD risk. Serum FGF23 levels were independently and positively associated with C-IMT. As a conclusion, serum FGF23 levels strengthen the capacity of the FRS for identifying subclinical atherosclerosis.

AI-P-35
Low-frequency and very low-intensity ultrasound decreases blood pressure in hypertensive subjects with type 2 diabetes K. Nonogaki

Department of Diabetes Technology, Tohoku University Graduate School of Biomedical Engineering
Despite lifestyle interventions and anti-hypertension agents, hypertension remains difficult to control in some patients with type 2 diabetes. Here we show the effects of low-frequency and low-intensity ultrasound applied to the forearm on blood pressure (BP) and pulse rate in Japanese subjects with type 2 diabetes and hypertension. Systolic and diastolic BP, pulse rate, pulse pressure in the ultrasound treatment group were significantly lower than the baseline values in hypertensive subjects with type 2 diabetes, and lower than those of placebo controls. Low-frequency and lowintensity ultrasound irradiation to the forearm might have potential usefulness as a therapeutic application for clinic hypertension in subjects with type 2 diabetes.

AI-P-36
Negative pressure wound therapy inhibit inflammation in diabetic patients with foot ulcerations Hypertension causes neuropathy in spontaneous hypertensive rats (SHR) (Nukada, et al., Muscle Nerve 2016). We studied the potential for the additive effects of diabetes upon existing hypertension for the development of neuropathy. Neonatal STZ-diabetes was induced in SHR and Wistar rats. SHR complicated with diabetes (SHR+DM), hypertension only (SHR), diabetes only (DM), and two controls; WKY and Wistar rats were studied. Nerve conduction velocity in SHR+DM was the slowest at age of 28 and 44 weeks. Morphological abnormalities, e.g. axonal atrophy of myelinated fibers, in SHR+DM was greater than in SHR or DM. These findings suggest that diabetes contributes to worsening of hypertensive neuropathy.

AI-P-38
Factors associated with post discharge follow up among hospitalized patients with diabetic foot This study aims to identify clinical and demographic factors associated with post-discharge follow up among patients admitted for one of the most common complications of diabetes, the diabetic foot.
Records of adults admitted for diabetic foot in the Philippine General Hospital from December 2011 to 2013 and subsequently discharged to the outpatient clinics were reviewed. Multiple logistic regression was done to derive associations between clinical, demographic variables and follow up.
Post-discharge follow up rate is 22%. Among all other variables, non-drinking status is associated with post-discharge follow-up (OR 1.98; CI 1.01 -3.88; P = .047). and may be a marker for good treatment-seeking behavior in this population.

AI-P-41
Screening for diabetic autonomic neuropathy using a blood pressure/ pulse wave measuring apparatus

N. Kato, M. Kato and H. Toshima Kato Clinic of Internal Medicine
We developed a simple test of autonomic function in diabetes. MATERIALS AND METHODS: In 77 patients with diabetes and 22 healthy volunteers, the cardio-ankle vascular index (CAVI, an index of peripheral vessel stiffness) was measured on a blood pressure/pulse wave apparatus (Vasera 1500, Fukuda Denshi). The standard deviation /mean ratio for the height of 5 consecutive pressure pulse waves (CVWH) was used to assess sympathetic function. RESULTS: CAVI was significantly higher and CVWH was significantly lower in the diabetes group (both P = 0.0001). In patients on oral agents, CVWH was positively correlated with vibratory sensation and negatively correlated with CAVI. SUMMARY: CVWH could be a screening test for diabetic autonomic neuropathy.

AI-P-42
Comparison of uric acid levels and mortality and risk of renalreplacement therapy in DM and non-DM CKD patients Hyperuricemia is associated with CVD, mortality, renal outcome and the metabolic syndrome. However, the cut-off value is unknown in DM and non-DM CKD patients.
We enrolled 1776 DM patients and 2605 non DM patients with CKD stage 3, 4, 5 from 2007 to 2014. The end-point was all cause mortality and receipt of renal-replacement therapy (RRT).
Results suggest that in patients with DM and CKD stage 3, 4, 5, the risk of all cause mortality is not increased when serum uric acid levels are <8.0 mg/dl. However, in patients without DM, all cause mortality is not increased when serum uric acid levels are <8.0 mg/dl. The risk of RRT was associated with increased serum uric acid levels. However, no difference in risks of RRT was noted in the two groups.

AI-P-43
Hemorheological approach for screening diabetic nephropathy in type 2 diabetes AI-P-45 Aberrant STRA6 and CRBP1 signal pathway is involved in development of diabetic nephropathy via mitochondrial dysfunction S. S. Jang 1,2 and C. C. Hung 1,2 Reduction of retinoids, CRBP1 in db/db mice kidney was found. Mitochondrial dysfunction ian initiator of DKD. We hypothesized aberrant STRA6 and CRBP1 contribute to DKD via mitochondrial dysfunction. We found that binding activity of RBP4 with STRA6, CRBP1, retinol, RA, RARa, and mitochondrial function (MnSOD, cytochrome c, ATP synthase, mitochondrial potential) decreased but caspase 3 and collagen 1 increased in DM kidney and HG-cultured HK-2 cells. CRBP1 transfection recovered STRA6 signal and renal injury in HG-cultured HK2 cells. ROS inhibitor and MnSOD transfection rescued aberrant STRA6 signal and renal injury. This study indicates that aberrant STRA6 and CRBP1signaling involve the process of DKD via mitochondrial dysfunction.

AI-P-46
The association of a genetic variant in SCAF8-CNKSR3 with diabetic microvascular disease in a Chinese population L. Jin, C. Hu and W. Jia

Shanghai Diabetes Institute
Genomewide association studies found rs955333 was associated with DKD in EA, AA and MA. We aimed to investigate the association between the variant rs955333 with DKD susceptibility in 1884 Chinese T2D patients. Associations of the variant rs955333 with DKD as well as DR susceptibility and related quantitative traits were evaluated. The variant rs955333 was not associated with DKD in our samples, while subjects with genotype GG were associated with DR (P = 0.047, OR = 0.5525[0.308, 0.9911]), and it also showed association with AER (P = 0.024, Beta = -0.1812 [-0.339, -0.024]). Our data suggests the variant rs955333 was not associated with DKD but showed association with DR in the Chinese T2D patients.

AI-P-47
Abnormal thermal thresholds of lower extremities is significantly associated with low TBI in type 2 diabetic patients We enrolled 725 type 2 diabetic patients with quantitative sensory test for thermal thresholds and ankle-brachial index/ toe-brachial index (TBI) examinations. Excluding patients with a history of apparent cardiovascular disease, arrhythmia, end-stage renal disease, malignancy, and diagnosed peripheral neuropathy. A total of 539 study subjects had impaired thermal thresholds. After adjusting for gender and age, abnormal thermal thresholds was significantly associated with low TBI and low eGFR (P < 0.05). Fasting plasma glucose and HbA1C remain independently associated with impaired thermal thresholds after adjusting for confounding factors. Abnormal sensory test of lower limbs is associated with peripheral artery disease in type 2 diabetes.

AII-P-1
Analysis of the Syndecan-4 gene expression control mechanism in MIN6 cell I. Takahashi, K. Yanase, S. Hatakeyama, T. Mizokami and K. Nata Department of Medical Biochemistry, School of Pharmacy, Iwate Medical University Heparan sulfate (HS) proteoglycans (PGs) comprise a core protein to which extracellular sugar chains are attached. We recently found that HS core protein Syndecan-4 (Sdc4) are necessary for maintaining insulin secretory mechanism. So analysis of the regulation of Sdc4 expression is important for better understanding diabetes. We analyzed the sequence, methylation in Sdc4 promoter, and executed the deletion reporter assay using Sdc4 expressing and non-expressing mouse pancreatic b-cell line, MIN6 cells. Sdc4 promoter sequences showed no change in both cells, while specific methylation on the promoter was detected in Sdc4 non-expressing cells only. Furthermore, deletion analysis revealed two important promoter regions for Sdc4 expression. We have developed a highly efficient step-wise protocol to direct insulin-producing cell from diabetes patient-specific iPS cell lines. For differentiation into insulin producing cells, we divided into 4 stages. We regulated the glucose concentration and used several small molecules in each steps and adenovirus for Pdx-1 overexpression in the stabilizing stage. In final step, we reaggregated the cells like an islet cluster. Interestingly, aggregated cells showed expression of insulin gene and insulin positive cells. To functional test, we performed glucose-stimulated insulin secretion. Interestingly, insulin secretion was a statistically significantly increased by high glucose treatment in differentiated cells.

AII-P-3
Generation of the beta-like cells differentiated into the Insulinproducing cells from Mouse small intestine. Aim of our study was to generate the insulin-producing cells form small intestine using an adenoviruses (Pdx-1, MafA, Beta2/NeuroD, (PMB)). Adenoviruses delivered from duodenum to colon in whole intestine, and identified infection with the Ad-Pdx1 and triple Ad-PMB respectively in mouse small intestine. At 4 weeks after injection, insulin mRNA expressed in small intestines. In additionally, insulin+ cells detected in a part of crypts and villus located of small intestine tissues by immunostaining. These data indicated that Pdx-1, MafA, Beta2/Neu-roD facilitate the differentiation into insulin producing cells of mouse intestinal cells. In conclusion, small intestine is an accessible and abundant source of insulin-producing cells.

AII-P-4
Chronic arsenic exposure impairs pancreatic b-cell function MIN6-K8 b-cell line, with no effects observed during acute (30 min) exposure. These studies raise the plausibility that arsenic exerts its diabetogenic effects through b-cells.

AII-P-5
Small molecules exert anti-apoptotic effect on ICAs B. Chandravanshi and R. Bhonde School of Regenerative Medicine, Manipal University Transplantation of pancreatic islets is the most reliable treatment for Type 1 diabetes. However cell death mediated by hypoxia is considered as one of the main difficulties hindering success in islet transplantation. The aim of our experiment was to investigate the role of small molecules in survival of Islet like cell aggregates (ICAs) engineered from umbilical cord matrix under oxygen deprived condition (<5%O2). ICAs were analyzed for cell death via fluorosceindiacetate/ propidium iodide (FDA/PI) staining, estimation of Caspase 3 and free radical release in presence and absence of small molecules. The samples were also analysed for the presence of hypoxia inducible factor 1a (HIF1a) at both transcriptional and translational level. The addition of small molecules showed profounddefensive effect on ICAs under hypoxicenvironment as evidenced by their viability and insulin secretion compared to untreated ICAs. The combinations of Eicosapentaenoic acid (EPA), Docosahexaenoicacid (DHA) and metformin and EPA,DHAandc amino butyric acid (GABA) acted as anti-apoptotic agentsforhuman ICAs when exposed to 1% O2 for 48 h. The combinations of the small molecules reduced the total reactive oxygen species and malonaldehyde (MDA) levels and enhanced the production of glutathione peroxidise (GPx) enzyme under hypoxic conditions. Finally the increase in HIF1a at both protein and gene level confirmed the defensive effect of the additives in hypoxia. These results suggest that the combination of small molecules maintained the viability and functionality of the ICAs in hypoxia by up-regulating HIF1a expression and down regulating the Caspase 3 activity.

AII-P-6
Whole exome sequencing identifies a novel INS mutation as a hot spot mutation of maturity-onset diabetes of the young We aimed to discover novel mutations leading to special types of diabetes which are misdiagnosed as type 2 diabetes in the Chinese population, and explore potential molecular mechanisms. We performed whole-exome sequencing in 31 'T2D' patients and found a novel mutation p.Ala2Thr in INS gene as a hot spot mutation of MODY. This variant was observed in 0.09% of cases in the replication study. Functional studies in INS1-E cells showed that this mutation impaired beta cell function through inducing endoplasmic reticulum stress.

AII-P-7
ATP and Ca 2+ dynamics in pancreatic b-cells: ex vivo analysis using ATP biosensor GO-ATeam1 ATP dynamics in b-cells was evaluated by ATP biosensor GO-ATeam1. The dissociated islet cells were subjected to fluorescent microscopy to evaluate ATP and Ca 2+ dynamics simultaneously during GIIS. The whole islets were subjected to two-photon excitation microscopy to evaluate ATP dynamics in individual bcells. ATP increased by 1.2-fold as early as~60s after glucose was increased; with Ca 2+ elevation~80s after the ratio increase. In the whole islets, the average ratio increased by 1.1-fold when glucose was increased. The ratio was increased iñ 30% of the cells examined. ATP rise proceed Ca 2+ elevation in b-cells during GIIS. Intracellular ATP did not elevate in some b-cells, suggesting heterogeneity of b-cells' response to glucose. AII-P-8 GPR120 mediated pdx1 signaling protects against palmitic acid-induced pancreatic beta-cell dysfunction Y. Wang and P. S. Leung School of Biomedical sciences, The Chinese University of HongKong As an unsaturated FFA sensor,GPR120 action in beta cells remains unexplored. Our results showed that GPR120 was expressed in beta cells and its stimulation with DHA or GSK restored palmitic acid (PA) downregulated the expression of insulin and pdx1 in MIN6 and islets. But the pdx1 level could not be rescued within GPR120 knockdown cells. In vivo,GPR120 KO mice displayed hyperglycemia and insulin resistance. We found that the pdx1 expression was decreased and the effects of GPR120 activation were not seen in GPR120 KO mouse islets compared with WT islets. The data suggests that GPR120 is protective against PA-induced beta-cell dysfunction via the mediation of pdx1.

AII-P-9
Sphingosine kinase 1-interacting protein (SKIP) is a novel regulator of glucose-stimulated insulin secretion We have reported that RNF34 is a bona fide E3 ubiquitin ligase for PGC-1a and negatively regulates brown fat cell metabolism, but whether RNF34 plays a role as an E3 ligase for PGC1-a in muscle is still unknown. Here, we show that tissue-specific knockdown of the Drosophila RNF34 in muscle increases mitochondrial DNA content, the mRNA levels of the dPGC-1 target genes and the negative geotaxis ability, reduces Drosophila dilp3 mRNA level and counteracts high-fat-diet induced high abdominal triglyceride amount. Knockdown of dPGC-1 suppresses the dRNF34 knockdown phenotypes, which suggests the effects of dRNF34 are mediated through dPGC-1. Our results might provide a new therapeutic approach for treatment of diabetes.

AII-P-15
Analysis of age-dependent leptin-signaling in the leptin receptor deficient mice Leptin is known to regulate energy homeostasis and food intake regulation in adult phase, but this function is scarcely investigated in neonatal period. We examined the age-dependent leptin-signaling dynamics in leptin receptor deficiency (db) mice. The body weight changes of db mice were occurring in adulthood. The impairment of db mice thermogenesis has shown in neonatal period and adulthood. The hypothalamus feeding neuron gene expression in neonatal db mice showed similar to wild type of mice. Leptin receptor is fully functional for energy homeostasis and food intake regulation in adulhood. However, the feeding regulation of leptin signaling shows age-dependent manner,in contrast to energy homeostasis regulation.

Institute for Cardiovascular Science of Soochow University
Diabetic cardiomyopathy is the leading cause contributing to morbidity and mortality in diabetic patients. We and others have demonstrated cardioprotection of follistatin-like 1 (FSTL1) under excessive nitric oxide, myocardial infarction, and pressure overload. Here, we report that high glucose markedly elevates FSTL1 expression in H9c2 cells. Furthermore, FSTL1 treatment significantly attenuates hyperglycemia-induced cardiac cell death. Our study provides novel insights into the therapeutic potential of FSTL1 in treatment of diabetic cardiomyopathy.

AII-P-37
Nurses' implementation and opinion of assessment of oral health behavior in patients with diabetes Diabetes Therapeutics and Research Center, Tokushima University AIMS: To describe (1) nurses' implementation of assessment of diabetes oral health behavior tool in patients with diabetes and (2) their opinion on the items of DiOHAT©, and discuss how to help the patients carry out oral health behavior. METHODS: Self-written questionnaire. RESULTS: Response rate 48%;15 female nurses; mean age 46 yr. The average rate of implementation of all items was 44% and individual factors were perceptions 56%, status 49%, behavior 39%, and information 37%. The proportion counting the patient's total number of teeth was 40%. CONCLUSION: The implications of assessing oral self-care and counting the number of teeth showed low rates. More research is needed to motivate nurses to carry out oral assessments.