Abstract
The influence of γ-ray irradiation on a cementum-impregnated gelatine membrane (CGM) was analyzed with emphasis on its function during periodontal regeneration. In brief, proteins were extracted kom γ-ray irradiated cementum (γC). With the γC protein, sample cells (gingival fibroblasts, periodontal ligament cells, and alveolar bone cells) were co-cultured, and cytological parameters (cell attachment, cell differentiation and alkaline phosphatase activity) were analyzed. Additionally, kinetics of some gene expression was analysed using reverse transcript RT-PCR, which included osteoproteogerin (OPG) / osteoclastogenesis inhibitory factor (OCIFT) mRNA BMP-2 and osteonectin were resistant to γ-rays, and other cytokines involved in regeneration were decreased. Thus, the attachment activity of osteoblasts to γC protein was higher than that of non-irradiated cementum (control C). The expression of OPG/OCIF mRNA was lower in co-cultured cells with γC protein than those with in control C protein. Together the results imply that some cytokine in intact cementum prevents the attachment (differentiation) of bone cells onto the root surface, which may explain why the introduction of CGM following gingival flap surgery induces new cementum, new ligament and new bone formation, but CGM irradiated with γ-rays for clinical use causes ankylosis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Lynch SE, Nixson JC, Colvin RB, et al.: Role of platlet-derived growth factors in wound healing: synergistic effects with other growth factors. Proc Natl Acad Sci USA. 84: 7696–7700, 1987.
Lynch SE, Williams RC, Polson AM: A combination of platlet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol. 16 545–548, 1989.
Somerman MJ, Sauk JJ, Foster RA, et al.: Cell attachment activity of cementum: bone sialoprotein II identified in cementum. J Periodontal Res. 26: 10–16, 1991.
Somennan MJ, Prince CW, Butler WT, et al.: Cell attachment activity of the 44 kilodalton bone phosphoprotein is not restricted to bone cells. Matrix. 9: 49–54. 1989.
Somerman MJ, Foster RA, Imm GM: Periodontal ligament cells and gingival fibroblasts respond differently to attachment factors in vitro. J Periodontol. 60 73–77, 1989.
D’Errico JA, Sank JJ, Prince CW, et al.: Osteopontin adhesion receptors on gingival fibroblasts. J Periodontal Res. 30: 34–41, 1995.
McAllister B, Narayanan AS, Miki Y, et al.: Isolation of fibroblast attachment protein from cementum. J Periodontal Res. 25: 99–105, 1991.
Wu D, Ikezawa K, Parker T, et al.: Characterization of a collagenous cementum-derived attachment protein. J Bone Miner Ŕes. 11: 686–692, 1996.
Liu HF, Yacobi R, Savion N, et al.: A collagenous cementum-derived attachment protein is a marker for progenitors of the mineralized tissue-forming cell lineage of the periodontal ligament. J Bone Miner Res. 12: 1691–1699, 1997.
Metzger Z, Weinstock B, Dotan M, et al.: Differential chemotactic effect of cementum attachment protein on periodontal cells. J Periodontal Res. 33: 126–129, 1998.
Nishimura K, Hayashi M, Shigeyama Y, et al.: The chemoattractive potency of periodontal ligament,cementum and dentin for human gingival fibroblasts. J Periodontal Res. 24: 146–148, 1989.
Nishimura K, Onodera Y, Takada K, et al.: Purification and partial characterization of bovine cementum-derived chemotactic factor. J Clin Periodontol. 27: 100, 2000 (abstr,).
Ogata Y, Niisato N, Moriwaki K, et al.: Cementum, root dentin and bone extracts stimulate chemotactic behavior in cells from periodontal tissue. Comp Biochem Physiol. 116: 359–365, 1997.
Miki Y, Narayanan AS, Page RC: Mitogenic activity of cementum components to gingival fibroblasts. J Dent Res. 66: 1399–1403, 1987.
Yonemura K, Narayanan SA, Page RC, et al.: Isolation and partial characterization of a growth factor from human cementum. Bone Miner. 18: 187–198, 1992.
Nishimura K, Kitamura H, Naito M, et al.: Cementum-impregnated gelatine membrane: Its effect on periodontal tissue regeneration. J Biomed Mater Res. 29: 227–232, 1995.
Nishimura K, Noguchi Y, Fukase Y, et al.: Effects of cementum-impregnated gelatine membrane on early healing following periodontal flap surgery. J Electron Microsc. 44: 91–99, 1995.
McCulloch CA, Nemeth E, Lowenberg B, et al.: Paravascular cells in endosteal spaces of alveolar bone contribute to periodontal ligament cell population. Anat Rec. 219: 233–242, 1987.
Somerman MJ, Archer SY, Imm GR, et al.: A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res. 67: 6670, 1988.
Falk W, Goodwin RH-J, Leonard EJ: A 48-well micro chemotaxis assembly for rapid and accurate measurment of leukocyte migration. J Immunol Methods. 33: 239–247, 1980.
Hou LT, Liu CM, Lei JY, et al.: Modulation of periodontal ligament fibroblasts by cementum and alveolar bone extracts. J Formos Med Assoc. 94: 401–405, 1995.
Cory AH, Owen TC, Barltrop JA, et al.: 1991 Use of an aqueous soluble tetrazolium/ formazan assay for cell growth assays in culture. Cancer Commun. 3: 207–212, 1991.
Cone JL, Brown DR, DeLarco JE: An improved method of purification of transforming growth factor, type β from platelets. Anal Biochem. 168: 71–74, 1988.
Lucas C, Bald LN, Fendly BM, et al.: The autocrine production of transforming growth factor-β 1 during lymphocyte activation. A study with a monoclonal antibody-based ELISA. J Immunol. 145: 1415–1422, 1990.
Bradfford MA: Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248–254, 1976.
Sakata M, Shiba H, Komatuzawa H, et al.: Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells. J Bone Miner Res. 14: 1486–1492, 1999.
Wada N, Maeda H, Tanabe K, et al.: Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function: the factor is osteoprotegerin/osteoclastogenesis inhibitory factor. J Periodontal Res. 36: 56–63, 2001.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nishimura, K., Tsuciya, H., Takada, K. et al. Effects of irradiation on cementum matrix cytokins function during periodontal regeneration. Hum Cell 16, 217–229 (2003). https://doi.org/10.1111/j.1749-0774.2003.tb00156.x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1111/j.1749-0774.2003.tb00156.x