2003 Volume 69 Issue 6 Pages 1194-1199
An enzyme biosensor system was developed for rapid and continuous determination of total cholesterol in fish plasma. The system was constructed with an immobilized enzyme membrane, Clark-type oxygen electrode, flow system and personal computer. The enzyme membrane was prepared from cholesterol esterase, cholesterol oxidase, photosensitive polymer and an ultra-thin dialysis membrane. The optimum assay conditions of the sensor system were established as follows: pH 6.8, temperature 25°C and flow rate 0.2 mL/min. The calibration curves were linear in the range of 15-80 mg/dL for yellowtail, 17-42 mg/dL for amberjack and 20-70 mg/dL for rainbow trout. One assay was completed within 5 min and it was possible to determine the sample continuously. The sensor system was applied to the determination of total cholesterol in yellowtail plasma. A good correlation was observed between the values determined by the sensor method and the conventional method (correlation coefficient was 0.90).
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