Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIα and IIβ genes of Japanese flounder Paralichthys olivaceus

PRAPANSAK SRISAPOOME; TSUYOSHI OHIRA; IKUO HIRONO; TAKASHI AOKI

doi:10.1111/j.1444-2906.2003.00800.x

Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIα and IIβ genes of Japanese flounder Paralichthys olivaceus

PRAPANSAK SRISAPOOME, TSUYOSHI OHIRA, IKUO HIRONO, TAKASHI AOKI

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Keywords: antigen presentation, evolution, fish immunity, Japanese flounder, major histocompatibility complex genes

JOURNAL FREE ACCESS

2004 Volume 70 Issue 2 Pages 264-276

DOI https://doi.org/10.1111/j.1444-2906.2003.00800.x

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Abstract

The nucleotide sequences of Japanese flounder Paralichthys olivaceus, major histocompatibility complex (MHC) cDNA, classical MHC class Iα, non-classical MHC class Iβ, MHC class IIα and IIβ, were determined. The domain structures and antigen binding motifs of vertebrate MHC are conserved in the Japanese flounder MHC. A phylogenetic analysis supports the classification of these genes into class I and class II MHC. Classical MHC class Iα was ubiquitously expressed, whereas the non-classical MHC class Iβ was expressed mainly in lymphoid organs, gills, intestine and stomach. The MHC classes IIα and IIβ were also ubiquitously expressed.

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