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Recording cerebellar neuron activities in swimming goldfish

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Abstract

Neuronal activities were investigated in the cerebellum of immobilized and swimming goldfish Carassius auratus. Extracellularly recorded neural activities of the cerebellum in immobilized goldfish were characterized and classified into five types. Based on the waveforms and recording depths, these five neural activity types were estimated to originate from three identified classes of cerebellar neurons: Purkinje cells eurydendroid cells, and granule cells. Chronic recording of cerebellar neuron activities in unrestrained goldfish was performed for more than 100 h. During the chronic recordings, a submersible amplifier attached to the goldfish head, and a multielectrode array developed for the present study were used. Neuronal activities in the cerebellum of free-swimming fish could also be classified into five types as in the immobilized condition. Firing patterns of two neurons identified as Purkinje cells and eurydendroid cells were analyzed during turning movements of the goldfish. The firing patterns of these neurons changed in relation to turning movements. Although some improvements are required, the chronic recording method developed in the present study can be applied to further investigations concerning the direct relationship between brain neural activities and certain behavior.

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References

  1. Rousche PJ, Normann RA. Chronic recording capability of Utah Intracortical Electrode Array in cat sensory cortex. J. Neurosci. Methods 1998; 82: 1–15.

    Article  PubMed  CAS  Google Scholar 

  2. Sakurai Y. Coding of auditory temporal and pitch information by hippocampal individual cells and cell assemblies in the rat. Neuroscience 2002; 115: 1153–1163.

    Article  PubMed  CAS  Google Scholar 

  3. Takahashi S, Sakurai Y. Real-time and automatic sorting of multi-neuronal activity for sub-millisecond interactions in vivo. Neuroscience 2005; 134: 301–315.

    Article  PubMed  CAS  Google Scholar 

  4. Enger PS. The electroencephalogram of the codfish (Gadus callarias). Acta Physiol. Scand. 1957; 39: 55–72.

    Article  PubMed  CAS  Google Scholar 

  5. Karmonova IG, Belich AI, Lazarev SA. An electrophysiological study of wakefulness and sleep-like states in fish and amphibians. In: Laming PR (ed.), Brain Mechanisms of Behaviour in Lower Vertebrates. Cambridge University Press, Cambridge, 1981; 181–202.

    Google Scholar 

  6. Laming PR. Electroencephalographic studies on arousal in the goldfish (Carassius auratus). J. Comp. Physiol. Psych. 1980; 94: 238–254.

    Article  CAS  Google Scholar 

  7. Karamian AI, Fanardjian VV, Kosareva AA. The functional and morphological evolution of the cerebellum and its role in behavior. In: Llinàs R (ed.), Neurobiology of Cerebellar Evolution and Development. American Medical Association, Chicago, IL, 1969; 639–673.

    Google Scholar 

  8. Roberts BL, van Rossem A, de Jager S. The influence of cerebellar lesions on the swimming performance of the trout. J. Exp. Biol. 1992; 167: 171–178.

    PubMed  CAS  Google Scholar 

  9. Tuge H. Study on cerebellar function in the teleost. I. Reactions resulting from cerebellar ablation. J. Comp. Neurol. 1934; 60: 201–224.

    Article  Google Scholar 

  10. Baba Y, Kake Y, Yoshida M, Uematsu K. Activities of mesencephalic nucleus neurons during fictive swimming of the carp Cryprinus carpio. Fish. Sci. 2003; 69: 581–588.

    Article  CAS  Google Scholar 

  11. Uematsu K, Ikeda T, Ohta K, Muromoto C. Fictive swimming induced by electrical and tactile stimulations in carp. Fish. Sci. 1994; 60: 533–535.

    CAS  Google Scholar 

  12. Yoshida M, Matsuura K, Uematsu K. Developmental changes in the swimming behavior and underlying motoneuron activity in the larval angelfish, Pterophyllum scalare. Zool. Sci. 1996; 13: 229–234.

    Article  Google Scholar 

  13. Peterson RH. Electrical responses of goldfish cerebellum, responses to parallel fibre and peduncle stimulation. Brain Res. 1972; 41: 67–79.

    Article  PubMed  CAS  Google Scholar 

  14. Ikenaga T, Yoshida M, Uematsu K. Cerebellar efferent neurons in teleost fish. Cerebellum 2006; 5: 268–274.

    Article  PubMed  CAS  Google Scholar 

  15. Humphrey DR, Schmidt EM. Extracellular single-unit recording methods. In: Boulton AA, Baker GB, Vanderwolf CH (eds). Neuromethods, Vol. 15. Humana Press. Clifton, NJ. 1990; 1–64.

    Google Scholar 

  16. Kotchabhakdi N. Functional circuitry of the goldfish cerebellum. J. Comp. Physiol. 1976; 112: 47–73.

    Article  Google Scholar 

  17. Welsh JP, Schwarz C. Multielectrode recording from the cerebellum. In: Nicolelis MAL (ed.). Methods for Neural Ensemble Recordings. CRC Press, Boca Raton, FL. 1998; 79–100.

    Google Scholar 

  18. Finger TH. Organization of the teleost cerebellum. In: Northcutt RG, Davis RE (eds). Fish Neurobiology. The University of Michigan Press. Ann Arbor, MI. 1989;261–284.

    Google Scholar 

  19. Meek J, Nieuwenhuys R. Holostean and teleosts. In: Nieuwenhuys R, ten Donkelaar HJ, Nicholson C (eds): The Central Nervous System of Vertebrates, Vol. 2. Springer-Verlag, Berlin. 1998; 759–935.

    Google Scholar 

  20. Lee LT, Bullock TH. Cerebellar units show several types of early responses to telencephalic stimulation in catfish. Brain Behav. Evol. 1990; 35: 278–290.

    Article  PubMed  CAS  Google Scholar 

  21. Han VZ, Bell CC. Physiology of cells in the central lobes of the mormyrid cerebellum. J. Neurosci. 2003; 23: 11147–11157.

    PubMed  CAS  Google Scholar 

  22. Laming PR. An introduction to the functional anatomy of the brains of fish and amphibians. In: Laming PR (ed.). Brain Mechanisms of Behaviour in Lower Vertebrates. Cambridge University Press, Cambridge. 1981; 7–32.

    Google Scholar 

  23. Ikenaga T, Yoshida M, Uematsu K. Efferent connections of the cerebellum of the goldfish. Carassius auratus. Brain Behav. Evol. 2002; 60: 36–51.

    Article  Google Scholar 

  24. Meek J, Nieuwenhuys R, Elsevier D. Afferent and efferent connections of cerebellar lobe C1 of the mormyrid fish Gnathonemus petersi: an HRP study. J. Comp. Neurol. 1986; 245: 319–341.

    Article  PubMed  CAS  Google Scholar 

  25. Meek J Nieuwenhuys R, Elsevier D. Afferent and efferent connections of cerebellar lobe C3 of the mormyrid fish Gnathonemus petersi: an HRP study. J. Comp. Neurol. 1986; 245: 342–358.

    Article  PubMed  CAS  Google Scholar 

  26. Wullimann MF, Northcutt RG. Connections of the corpus cerebelli in green sunfish and the common goldfish: a comparison of perciform and cyprimiform teleosts. Brain Behav. Evol. 1988; 32: 293–316.

    Article  PubMed  CAS  Google Scholar 

  27. Dave AS, Yu AC, Gilpin JJ, Margoliash D. Methods for chronic ensemble recordings in singing birds. In: Nicolelis MAL (ed.). Methods for Neural Ensemble Recordings. CRC Press, Boca Raton, FL. 1998; 101–120.

    Google Scholar 

  28. Yoshida T, Mashimo T, Akagi M, Iwata A, Yoshida M, Uematsu K. A design of neural signal sensing LSI with multi-input-channels. IEICE Trans. Fundamentals 2004; E87A: 376–383.

    Google Scholar 

  29. Yoshida T, Akagi M, Mashimo T, Iwata A, Yoshida M, Uematsu K. Design of a wireless neural-sensing LSI. IEICE Trans. Electron. 2004; E87C: 996–1002.

    Google Scholar 

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Correspondence to Masayuki Yoshida.

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Matsumoto, N., Yoshida, M. & Uematsu, K. Recording cerebellar neuron activities in swimming goldfish. Fish Sci 73, 512–521 (2007). https://doi.org/10.1111/j.1444-2906.2007.01363.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01363.x

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