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The dimeric outer 5S subunit of transcarboxylase has been expressed in three different forms and crystallized: native 5S, 5S-His6 and selenomethione-5S-His6. All the crystals have an orthorhombic space group, but while native 5S forms primitive orthorhombic crystals, 5S-­His6 crystals are either C-centered or primitive and SeMet-5S-His6 crystals are C-centered. Crystallization of native 5S requires the addition of lithium sulfate, whereas this salt prevented crystallization of 5S-His6. All 5S crystals diffract to ∼2.0 Å resolution with synchrotron radiation. Efforts are under way to solve the structure of SeMet-5S-His6 using MAD.

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