Figure 1

Imaging hydrated specimens in wet CDI. Cells suspended in buffer solution are preserved between two membranes. The pinhole aperture and Si guard slits are shown along with the sample holder, which are all contained in an acrylic enclosure under helium ambiance.Reuse & Permissions

Figure 2

(a) Speckle pattern from a fully hydrated, unstained yeast cell. The center of the diffraction pattern in dark blue shows the blocked portion by the x-ray beam stopper, recovered during the phase retrievals. (b) Reconstructed image. The high-density region is likely the nucleus ($N$) with implied substructures. The projected electron density map is displayed in color with the scale bar to the right.Reuse & Permissions

Figure 3

(a) Speckle pattern obtained from a hydrated cyanobacterium. (b) Reconstructed image with discernible internal structures labeled as carboxysomes (CB), gas vesicles ($G$), and thylakoid membranes ($T$). Projected electron densities are displayed in color with the scale bar to the right. (c) TEM image of sectioned, stained cyanobacetrium. (d) Line plot along the lines in (b) visualizing clear image contrast and 30 nm feature of the thylakoid membrane at better than 50 nm resolution.Reuse & Permissions

Figure 4

(a) Cross correlation function estimated for a series of short time exposed speckle patterns from a yeast (triangle) and cyanobacterium (circle). Speckle patterns remain consistent up to the total exposure of $\sim 1\times {10}^8\mathrm{Gy}$. (b) Power spectral density (PSD, left axis) and phase retrieval transfer function (PRTF, right axis) of the accumulated speckle patterns of the yeast (black) and cyanobacterium (blue). Both of PSD and PRTF manifest that signals from the sample extend a spatial resolution better than 40 nm, or 20 nm in half period, with reliable phase retrieval.Reuse & Permissions