Identification of a Sex-factor-affinity Site in E. coli as γδ

  1. M. S. Guyer*,,
  2. R. R. Reed,
  3. J. A. Steitz, and
  4. K. B. Low§
  1. *Laboratory of Molecular Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, Bethesda, Maryland 20205; Department of Molecular Biophysics and Biochemistry and § Radiobiology Laboratories, Yale University, New Haven, Connecticut 06510

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Excerpt

Many conjugative bacterial plasmids are capable of promoting the conjugal transfer of normally physically unlinked replicons, including the main bacterial chromosome (for review, see Holloway 1979). Such behavior has been termed mobilization (Novick et al. 1976) or conduction (Clark and Adelberg 1962; Clark and Warren 1979). One model proposed to describe at least some such interactions involves recombination between regions of extensive nucleotide sequence homology on both the conjugative and nonconjugative replicons (Clark and Warren 1979). Such recombination-mediated processes occur in the cases of some experimentally altered plasmids (Wilkins 1969; Kleckner et al. 1977; Barth 1979).

Insertion sequence (IS) elements (for review, see Starlinger 1980 and this volume) are resident in many bacterial replicons, including the main chromosome as well as plasmids (Saedler and Heiss 1973; Deonier et al. 1979). Much attention has been focused on the ability of these genetic elements to mediate specialized recombination processes in which nucleotide...

  • Present address: The Genex Corporation, Bethesda, Maryland 20205.

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