Purification and Characterization of the SV40 F-gene Protein
This extract was created in the absence of an abstract.
Excerpt
Cells transformed by SV40 consistently express two early genes of the virus. Genetic approaches have confirmed that both these genes are required for the complete expression of the transformed phenotype.
The A gene codes for large T antigen, or the A-gene protein (Rundell et al. 1977), and is required to initiate and maintain all aspects of the transformed state under appropriate circumstances (Brugge and Butel 1975; Martin and Chou 1975; Osborn and Weber 1975; Tegtmeyer 1975; Rassoulzadegan et al. 1978). In productive infection, the A-gene protein regulates the initiation of viral DNA replication and the initiation of early and late transcription (Tegtmeyer 1972; Tegtmeyer et al. 1975; Reed et al. 1976). The mechanism of action of the A-gene protein is incompletely understood, but specific binding of the protein to viral DNA at the origin of replication and inherent ATPase activity may be important in these regulatory roles (Tjian 1978; Tjian...
