Fluorescent Cell Staining Methods for Living Hypsibius exemplaris Embryos
- 1Biology Department, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599;
- 2Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599;
- 3Embryology Course, Marine Biological Laboratory, Woods Hole, Massachusetts 02543;
- 4Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
- ↵5Correspondence: bobg{at}unc.edu
Abstract
The tardigrade Hypsibius exemplaris was chosen as a model system in part because animals and embryos are optically clear at all stages, facilitating the visualization of events in living material. Here we report new methods for introducing fluorescent dyes into developing H. exemplaris embryos, including methods for fluorescently marking mitochondria, lysosomes, membranes, and nuclei. The development of these techniques suggests approaches for attempting to introduce other molecules into embryos.
Footnotes
-
From the Emerging Model Organisms collection.
