Protocol

Labeling of DNA Probes by Polymerase Chain Reaction

  1. Joseph Sambrook

Abstract

The polymerase chain reaction (PCR) can be used to produce both nonradiolabeled DNA probes and radiolabeled DNA probes with high specific activity. In this protocol, PCR is used to generate double-stranded probes. Related methods, including the generation of asymmetric probes by PCR, are also discussed.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. Published in Advance December 14, 2021, doi: 10.1101/pdb.prot100610 Cold Spring Harb Protoc 2022: pdb.prot100610- © 2022 Cold Spring Harbor Laboratory Press
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