Protocol

Detecting Alkaline Phosphatase–Labeled Cells

  1. Scott J. Rodig

Abstract

Different substrates are available for detection of cells labeled with alkaline phosphatase. Naphthol-AS-BI-phosphate (NABP)/New Fuchsin produces an intense red product that is insoluble in alcohols as well as aqueous solutions and is compatible with a range of histochemical stains. Bromochloroindoyl phosphate/nitro blue tetrazolium (BCIP/NBT) is the most commonly used of the chromogenic substrates for alkaline phosphatase. It is somewhat more sensitive than NABP/New Fuchsin but gives less contrast during microscopic observation.

Footnotes

  • From the Antibodies collection, edited by Edward A. Greenfield.

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  1. doi:10.1101/pdb.prot099721 Cold Spring Harb Protoc 2019: pdb.prot099721- © 2019 Cold Spring Harbor Laboratory Press
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