Measurement of Intracellular Ca²⁺ Release in Intact Cells Using ⁴⁵Ca²⁺

Abstract

This protocol describes a technique using ⁴⁵Ca²⁺ to measure the release of Ca²⁺ from the intracellular stores in monolayers of intact cells cultured in 12-well 4-cm² clusters. The ⁴⁵Ca²⁺-flux technique described here can only be applied to cell types that adhere to plastic. We describe the loading of the stores with ⁴⁵Ca²⁺, and the subsequent ⁴⁵Ca²⁺ efflux.