In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p

  1. Nicholas A. Kent1,
  2. Nickoletta Karabetsou,
  3. Panagiotis K. Politis, and
  4. Jane Mellor
  1. Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK

Abstract

Isw1p and Isw2p are budding yeast homologs of the DrosophilaISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.

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Footnotes

  • ↵1 Corresponding author.

  • E-MAIL nakent{at}molbiol.ox.ac.uk; FAX 44-1-865-275297.

  • Article and publication are at www.genesdev.org/cgi/doi/10.1101/gad.190301.

    • Received September 18, 2000.
    • Accepted December 13, 2000.
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This Article

  1. doi: 10.1101/gad.190301 Genes & Dev. 15: 619-626 Cold Spring Harbor Laboratory Press

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