Structure and Function of the Transforming Genes of Human Adenoviruses and SV40
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Excerpt
Transformation by human adenoviruses is a process in which only a small fraction of the viral genome is involved. This is most clearly shown by the observation that specific DNA fragments originating from the left-hand end of the genome are able to transform cells in vitro (Graham et al. 1975; van der Eb et al. 1977; van der Eb and Houweling 1977). This is further supported by the finding that rodent cells transformed by human adenovirus types 2 or 5 (Ad2 or Ad5) all contain viral DNA sequences homologous to the left-hand 14% of the genome, whereas some of the lines also contain sequences homologous to other parts of the viral DNA (Gallimore et al. 1974; Sharp et al. 1975; Flint et al. 1976). This indicates that transformation is basically a function of early region 1 (E1), which maps between 1% and 11% in the DNA of all human adenoviral...
