Viral and Cellular Control of the SV40-transformed Phenotype

  1. J. B. Hiscott and
  2. V. Defendi
  1. Department of Pathology, New York University Medical Center, New York, New York 10016

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Excerpt

Cellular transformation by SV40 requires the stable inheritance of viral gene sequences and the interaction with infected host cells of two early virus-specified gene products. Temperature-sensitive point mutations (tsA) or deletion mutations within the A-gene locus of SV40 DNA alter the synthesis, stability, and function of a 94K DNA-binding phosphoprotein, large T antigen (Tegtmeyer 1972; Scott et al. 1976; Tegtmeyer et al. 1977; Tjian 1978). In SV40-infected permissive cells, the A-gene product exhibits multifunctional properties, including control of initiation of viral DNA synthesis (Tegtmeyer 1972; Chou et al. 1974), autoregulation of early viral mRNA synthesis (Alwine et al. 1977; Khoury and May 1977), stimulation of host macromolecular synthesis (Weil 1978), and induction of resting cells into the cell cycle (Hiscott and Defendi 1979). In addition, functional A-gene expression is required for initiation of cellular transformation, since infection of rodent cells with tsA mutants at the nonpermissive temperature for A-gene expression...

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