Temperature-Sensitive Mutants of B. subtilis Defective in DNA Synthesis
This extract was created in the absence of an abstract.
Excerpt
The synthesis of DNA in vitro using deoxynucleoside triphosphates as substrates and native or denatured DNA as templates for DNA polymerase has been studied in much detail (see Englund et al., this volume). However, it is still uncertain what role this system plays in the in vivo replication of DNA. It is also unclear what mechanisms are responsible for the initiation of replication at a unique site on the bacterial chromosome (Yoshikawa and Sueoka, 1963a; 1963b), and for coordinating the initiation of replication with cell growth and division (Maaløe and Kjeldgaard 1966). The isolation of temperature-sensitive mutants defective in DNA synthesis should be of use in determining the number of distinct functions involved in DNA replication, the nature of these functions, and their relation to other cellular processes.
A variety of temperature-sensitive mutants defective in DNA synthesis (tsDNA mutants) have been isolated in E. coli (Kohiyama et al., 1966; Bonhoeffer...
