Modification of sRNA and Regulation of Protein Synthesis
This extract was created in the absence of an abstract.
Excerpt
Degeneracy of the code has been established now beyond a reasonable doubt (Nirenberg et al., 1963; Speyer et al., 1963; Söll et al., 1965). The corresponding degeneracy (heterogeneity) in sRNA has been reported in leucyl-sRNA of E. coli (Weisblum, Benzer, and Holley, 1962; Bennett, Goldstein and Lipmann, 1963; Yamane, Cheng, and Sueoka, 1963; Leder and Nirenberg, 1964; Söll et al., 1965), and also in other aminoacyl-sRNAs (Kellogg et al., 1966; Zachau et al., this volume). Thus, the involvement of sRNA as the adaptor between an RNA template and a polypeptide chain predicts that this class of molecules should have at least three functional sites, not necessarily independent of each other. One site confers specificity for recognition by the corresponding aminoacyl-sRNA synthetase, another serves as an anticodon for hydrogen bonding to specific codon triplets on the template, and a third functions as a site for binding to the ribosome. None of...
