Additions to the list of arthropods of Reunion Island

. − Contribution to the inventory of arthropods of Reunion Island. This work is a synthesis of new taxa for the Reunion Island identified between 1963 and 2022 and not published to date. The collections were carried out mainly by researchers and technicians from CIRAD or FDGDON. Sampling mainly targeted at crop pests and their natural enemies. The list includes 101 taxa new for Reunion. The Hymenoptera and Hemiptera are the most represented orders.

The list of Insecta Linnaeus, 1758 and Arachnida Cuvier, 1812 from Reunion Island (21.12° S, 55.5°E) in 2021 included 3155 and 216 taxa respectively in the French national taxonomic reference system TAXREF (Gargominy et al., 2021).Legros et al. (2020) estimated, by statistical methods, the expected number of taxa in Reunion (taxa already known and taxa not yet reported) at 7673 for Insecta and 824 for Arachnida.These authors estimate that 62% of the terrestrial arthropod fauna of Reunion remains to be discovered.
The work presented here is a contribution to the inventory of arthropods in Reunion.The list is a synthesis of taxa not published to date, collected between 1963 and 2022 mainly as part of crop pest and beneficial identification activities.

MATERIALS AND METHODS
Material examined.− The collections were carried out over a period extending from 1963 to 2022.The collections mainly targeted crop pests and beneficials.The specimens were kept dry (before 2015) or in ethanol 95° to -30°C (from 2015).The specimens are stored in the collections of the CBGP (Centre de Biologie pour la Gestion des Populations) in Montpellier (https://doi.org/10.15454/D6XAKL)or in the collection managed by the CIRAD (Centre de Coopération Internationale en Recherche Agronomique pour le Développement) at the Pôle de Protection des Plantes (3P) in Saint-Pierre in Reunion Island.The specimens were morphologically identified by the co-authors of this work or sent to specialists for morphological identification (see details in Results section).Five taxa were identified exclusively by barcoding (see below) using the BIN (Barcode Index Number) assignment tool of the BOLD database (Ratnasingham & Hebert, 2013).
Sequencing. − The specimens collected from 2015 onwards were characterised by barcoding.
DNA extraction from the specimens was performed non-destructively using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Courtaboeuf, France).DNA samples are stored at 3P.
We sequenced a fragment at position 5' of the mitochondrial gene for Cytochrome c oxidase I (COI-5P) (Hebert et al., 2003).However, in the case of Pseudococcidae, difficulties in PCR amplification led us to amplify instead a portion at the 3' end of the gene (COI-3P).The positions of the amplified gene portion and the primer pairs used are detailed in Table I, the PCR conditions are indicated by the associated bibliographic references.The sequencing of PCR products was subcontracted to a private company.

RESULTS
List of equipment examined.− Specimens with a code in XXXXnnnnn_nn format can be consulted on the Arthemis online database (INRAE-CBGP, 2022), with the exception of those whose code begins with 'FAUN'.Unless otherwise stated, the specimens collected are imagos.In the absence of any mention of the collector or the number of specimens collected, these are unknown.

Table II .
List of 101 taxa new for Reunion Island.