Temporin B Forms Hetero-Oligomers with Temporin L, Modifies Its Membrane Activity, and Increases the Cooperativity of Its Antibacterial Pharmacodynamic Profile

The pharmacodynamic profile of antimicrobial peptides (AMPs) and their in vivo synergy are two factors that are thought to restrict resistance evolution and ensure their conservation. The frog Rana temporaria secretes a family of closely related AMPs, temporins A–L, as an effective chemical dermal defense. The antibacterial potency of temporin L has been shown to increase synergistically in combination with both temporins B and A, but this is modest. Here we show that the less potent temporin B enhances the cooperativity of the in vitro antibacterial activity of the more potent temporin L against EMRSA-15 and that this may be associated with an altered interaction with the bacterial plasma membrane, a feature critical for the antibacterial activity of most AMPs. Addition of buforin II, a histone H2A fragment, can further increase the cooperativity. Molecular dynamics simulations indicate temporins B and L readily form hetero-oligomers in models of Gram-positive bacterial plasma membranes. Patch-clamp studies show transmembrane ion conductance is triggered with lower amounts of both peptides and more quickly when used in combination, but conductance is of a lower amplitude and pores are smaller. Temporin B may therefore act by forming temporin L/B hetero-oligomers that are more effective than temporin L homo-oligomers at bacterial killing and/or by reducing the probability of the latter forming until a threshold concentration is reached. Exploration of the mechanism of synergy between AMPs isolated from the same organism may therefore yield antibiotic combinations with advantageous pharmacodynamic properties.

H ost defense peptides (HDPs) are multifunctional molecules that are key components of the innate immune system and are found in all classes of life. There is interest in developing HDPs for therapeutic use as part of the response to the global increase in antimicrobial resistance, with some peptides able to combat infections by influencing the host immune response and antimicrobial peptides (AMPs) possessing highly potent bactericidal activity. 1 Because of this and because, unlike clinically relevant antibiotics, HDPs and AMPs are produced by metazoans to counter infections, there is also interest in understanding how they have remained effective throughout evolutionary history. 2 In laboratory conditions, serial passage of, e.g., Staphylococcus aureus in the presence of AMPs leads to reduced susceptibility to both clinically prescribed antibiotics and human HDPs, and this can be achieved with no detectable impact on fitness. 3 An alternative perspective however is provided by work that has shown that, while adaptation to AMPs is indeed readily achievable, the resulting resistance levels are generally far lower than obtained with antibiotics under the same conditions. 4 The modifications that arise as a result of bacterial adaptation to AMPs include changes in membrane surface charge, potential, permeability and fluidity, and the production of outer membrane vesicles, and these may lead to altered susceptibility to HDPs, a reduction in host colonization, and increased stimulation of host macrophages. 2 It remains possible therefore that, as has been shown for mcr-1, 5 the trade-offs required between fitness and resistance are such that the resistance that is achievable against AMPs has a ceiling below that observed for antibiotics, in particular in an in vivo setting.
It is further suggested that the pharmacodynamics of AMPs reduces the probability of resistance emerging. 6 Antimicrobial agents that have a more cooperative, dose dependent activity, as characterized by a steeper slope in a dose−response curve, benefit from a narrow mutant selection window which results from a smaller concentration range where efficacy is incomplete. AMPs have, in general, a more cooperative dose−response than antibiotics and hence a smaller window in which a selective pressure will be exerted. 7 The chemical composition of the infection setting may play an important role in limiting the ability of pathogenic bacteria to adapt to the innate immune response by ensuring that multiple AMPs, with differing mechanisms of action, are available. Cross-resistance between AMPs with differing mechanisms of action has been shown to be low, 8 while combining AMPs with differing mechanisms of action, from different organisms, has been shown to further enhance the cooperativity of the dose− response 7 and hence the pharmacodynamic properties of combinations of AMPs may further limit the risk of resistance emerging. The extent to which combinations of AMPs from the same organism act in synergy and how they might interact to produce a more cooperative dose−response is however yet to be fully explored.
The temporins comprise a very well-studied family of AMPs that now number more than 130 peptides 9 and whose members have been extensively evaluated and engineered to gain superior antibacterial activity. 10 Temporin L is a broadspectrum AMP, with potent bactericidal activity, identified, along with nine further temporins, in the European red frog Rana temporaria. 11 Synergy against Gram-negative species has been described between temporin L and each of temporin A and temporin B which, individually, have only weak activity. 12 Temporin L was shown to disrupt homo-oligomerization of both temporin A and temporin B, behavior that would enhance their translocation across the outer membrane and access the bacterial plasma membrane, the presumed site of their membrane disruptive activity. 12 Temporins A and L differ substantially in their molecular mechanisms of action, 13 and our previous work has identified fundamental differences in how temporins B and L insert into, and induce ion conductance in, models of the bacterial plasma membrane. 14,15 Here we use two time-resolved biophysical methods, molecular dynamics (MD) simulations and patch-clamp, to examine how a combination of temporin B and temporin L inserts into and disrupts models of the Gram-positive plasma membrane. We use this understanding to explain how the less potent temporin B can influence the cooperativity of the dose dependent bactericidal activity of temporin L against methicillin resistant S. aureus, which is in-turn compared with that of existing, clinically relevant antibiotics. Together, this provides a mechanistic perspective of how AMPs from the same organism may combine to enhance the pharmacodynamic profile and consequently reduce the risk of resistance to the innate immune response emerging.
Antibacterial Activity Assay. The antibacterial activity of the peptides was assessed through a modified 2-fold broth microdilution assay with modal MICs generated from at least three biological replicate experiments. 14−16 The method broadly followed EUCAST methodology, with noncation adjusted Mueller Hinton replacing cation-adjusted Mueller Hinton. Peptides and antibiotics were diluted in a 2-fold dilution in media down a sterile, polypropylene 96 well plate (Greiner Bio-One GmbH, Frickenhausen, Germany). Bacteria were then added, back-diluted from an overnight culture, at a starting concentration of 5 × 10 5 CFU/mL. Plates were incubated, static at 37°C, for 20 h, and the OD 600 was determined using a Clariostar plate reader (BMG Labtech). The MIC was defined as the lowest concentration where growth was <0.1 above the background absorbance. For temporin B/temporin L synergy screening experiments, MICs were performed as above, but with molar ratios of the two AMPs, i.e., 1:1, 3:1, and 1:3 for temporin L/temporin B. To test for synergy between temporin L, temporin B, and their combination with buforin II, checkerboard assays were conducted under the same conditions as the MICs but in Luria−Bertani (LB). Doubling dilutions of the two components, first temporin L vs temporin B and subsequently temporin B/temporin L vs buforin II, were performed on two 96-well plates, one horizontally and one vertically. These were combined and bacteria were added as for the MIC. FIC is calculated as (MIC of compound A in combination with B/ MIC of compound A alone) + (MIC of compound B in combination with A/MIC of compound B alone). MICs were determined on the same plates as the FICs to increase reproducibility. FIC values ≤0.5 would be considered strongly synergistic and, consistent with a recent re-evaluation of FIC which stresses the importance of also measuring the MIC in the same microarray plate, values of 0.5 to < 1 were weakly synergistic. 17 EMRSA-15 (NCTC 13616) and all other strains have been sequenced to allow linkage of resistance phenotypes to known genetic traits.
In Vitro Pharmacodynamic Assay. In vitro pharmacodynamic assays were performed with epidemic methicillin resistant S. aureus 15 (EMRSA-15) (NCTC 13616) cultured in Mueller Hinton Broth (MHB). Cation adjusted MHB (CA-MHB) was used when testing daptomycin due to its requirement for Ca 2+ ions for activity. Bacteria were cultured overnight in 10 mL of MHB or CA-MHB at 37°C and diluted just prior to plate inoculation to an OD 600 of 0.002. Stock solutions of temporin B, temporin L, pleurocidin, tobramycin, or gentamicin were prepared in sterile Milli-Q water at a concentration of 200× MIC. Daptomycin was prepared in methanol at a concentration of 2000× MIC and diluted with media to 200× MIC in the first well. A dilution series was made in the top row of a polypropylene 96-well plate from 200× MIC to 0.2× MIC in a volume of 100 μL, to which 100 μL of the bacterial suspension was added to have a total of 1 × 10 6 log-phase colony forming units (CFU) in 200 μL. The first t = 0 sample was taken <30 s after addition of bacteria to the plate with further samples taken at appropriate intervals thereafter. Peptide challenged bacteria were sampled every 20 min for 120 min due to rapid killing while tobramycin, gentamicin, and daptomycin challenged bacteria were sampled every hour for 6 h. A volume of 15 μL was removed from each well and diluted 1:1000 in phosphate buffered saline and plated onto MH agar or CA-MH agar plates. The plates were incubated at 37°C overnight for CFU counting. CFU data Biochemistry pubs.acs.org/biochemistry Article were log 10 transformed, and the bacterial net growth rate was determined from the increase or decrease in CFU during the time of exposure to the peptides or antibiotics as the coefficient of a linear regression of log 10 CFU as a function of time. The intercept of the regression was fixed by forcing the regression lines through the first CFU measurement (0 min) at a given antimicrobial concentration. The pharmacodynamic function according to Regoes et al. 18 describes the relationship between bacterial net growth rate ψ and the concentration of an antimicrobial (a): Fitting this function to the net bacterial growth rates in OriginPro 2020 (OriginLab Corporation, Northampton, MA) generates parameters ψ min and ψ max , respectively, the minimum and maximum growth rate, zMIC, the pharmacodynamic minimum inhibitory concentration, and κ, a measure of the cooperativity. Average parameters obtained from fits of three or more independently repeated experiments were compared by one-way ANOVA with a Tukey posthoc test. Since the CFU data is log 10 transformed, the net growth rates, are thereafter reported to three significant figures Molecular Dynamics Simulations. Peptide starting structures were copies of the same conformer obtained from previous NMR calculations of peptide prepared in SDS micelles. 14,15 Structural coordinates in the Protein Data Bank (www.rcsb.org) are accessed using codes 6GS5 and 6GIL for temporin L and temporin B, respectively. Simulations were carried out on either the ARCHER Cray XC30 supercomputer or Dell Precision quad core T3400 or T3500 workstations equipped with a 1 kW power supply (PSU) and two NVIDA PNY GeForce GTX570 or GTX580 graphics cards using Gromacs. 19 The CHARMM36 all-atom force field was used in all simulations. 20,21 The initial bilayer configuration was built using CHARMM-GUI. 22 All membranes in this project contained a total of 512 lipids, composed of 1-palmitoyl-2oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG) to reflect the lipid charge ratios of the plasma membrane of Grampositive bacteria. 23,24 Eight peptides were inserted at least 30 Å above the lipid bilayer in a random position and orientation at least 20 Å apart. The system was solvated with TIP3P water, and Na + ions were added to neutralize the total charge of the simulated system. Energy minimization was carried out using the steepest descent algorithm until the maximum force was less than 1000.0 kJ/mL/nm (∼3000−4000 steps). Equilibration was carried out using the NVT ensemble for 100 ps and then a semi-isotropic NPT ensemble for 1000 ps with position restraints on the peptides. Hydrogen-containing bond angles were constrained with the LINCS algorithm. The production simulations were run using a semi-isotropic NPT ensemble using 2 fs timesteps, with trajectories recorded every 2 ps. All simulations were performed at a temperature of 310 K, which was controlled with a Nose−Hoover thermostat, and at a pressure of 1 bar, which was controlled with a Parrinello− Rahman barostat. All production simulations were run for a total of 200 ns and duplicated, with peptides inserted at different positions and orientations, giving a total of approximately 1.2 μs of simulation. To investigate the aggregation of the AMPs, we have identified peptides that have come within 6 Å of each other at any given time step to be clustered. The connected components algorithm of NetworkX was used to find connectivity using graph theory. To quantify the conformation of the peptides, we measure torsion angles which are circular quantities, and the circular mean of psi or phi angles may be calculated as follows: Similarly, the associated circular variance for psi or phi angles is calculated as follows: Electrophysiology Experiments (Patch-Clamp). Giant unilamellar vesicles (GUVs) composed of DPhPG were prepared in the presence of 1 M sorbitol by the electroformation method in an indium−tin oxide (ITO) coated glass chamber connected to the Nanion Vesicle Prep Pro setup (Nanion Technologies GmbH, Munich, Germany) using a 3-V peak-to-peak AC voltage at a frequency of 5 Hz for 140 min at 37°C. 25−27 Bilayers were formed by adding the GUVs solution to a buffer containing 250 mM KCl, 50 mM MgCl 2 , and 10 mM Hepes (pH 7.00) onto an aperture in a borosilicate chip (Port-a-Patch; Nanion Technologies) and applying 70−90 mbar negative pressure resulting in a solvent-free membrane with a resistance in the GΩ range. Diphytanoyl chains are used here for practical reasons since, unlike lipids with mixed palmitoyl-oleoyl chains such as POPG, these lipids do not undergo the main, temperature dependent transition from disordered fluid into the all trans configuration and remain in the same phase between −120°C and +120°C, 28 while crucially, the membranes composed of these lipids are mechanically stable and have high specific resistance, 29 essential for electrophysiology experiments. After formation, a small amount of peptide stock solution (in water) was added to 50 μL of buffer solution in order to obtain its active concentration. All the experiments were carried on with a positive holding potential of 50 mV. The active concentration, the concentration at which the peptide first showed membrane activity, for each peptide was obtained through a titration performed in the same conditions. For all the experiments, a minimum of six concordant repeats were done. Current traces were recorded at a sampling rate of 50 kHz using an EPC-10 amplifier from HEKA Elektronik (Lambrecht, Germany). The system was computer controlled by the PatchControl software (Nanion) and GePulse (Michael Pusch, Genoa, Italy, http:// www.ge.cnr.it/ICB/conti_moran_pusch/programs-pusch/ software-mik.htm). The data were filtered using the built-in Bessel filter of the EPC-10 at a cutoff frequency of 10 kHz. The experiments were performed at room temperature. Data analysis was performed with the pClamp 10 software package (Axon Instruments). Estimation of pore radii was performed as previously. 30 ■ RESULTS Temporin B Does Not Substantially Increase the Antibacterial Potency of Temporin L. FICs for the Biochemistry pubs.acs.org/biochemistry Article combination of temporin B and temporin L have been shown previously to be in the range of 0.55−0.75 for four Grampositive strains and from 0.41 to 0.50 for four Gram-negative strains with a conservative value of ≤0.50 considered to represent synergy due to the inherent uncertainty in brothmicrodilution assays. 12 More recently, some researchers have suggested that values in the range 0.50−0.99 can also represent synergy, albeit modestly so, if care is taken to obtain MICs and FICs from the same plate. 17 Values between 1.00 and 1.99 represent no interaction. In our previous studies, temporin L was shown to be more potent than temporin B against all strains included in both the Gram-positive and Gram-negative bacteria panels and Candida albicans (Table 1). 14,15 Here, to facilitate a rapid and efficient screen of synergy in the whole panel, rather than employing checkerboard assays, three fixed ratios of temporin L and temporin B are tested to generate a range of FIC for three different stoichiometries (Table 1). In general, no evidence of strong synergy is found, with only small reductions of the amount of temporin L needed to inhibit bacterial growth when used in combination with temporin B. In some cases, a reduction in the amount of temporin L required is obtained with the addition of a small amount of temporin B and, considering its low potency when used alone, this produced FICs below 1.00. Overall, however, the present and previous studies agree that, at best, only modest synergistic  14 and temporin L. 15 The MICs for temporin B and temporin L are given when used alone or in three combinations with differing stoichiometric ratios. The FIC range is the range of FICs obtained across the three differing stoichiometric ratios.  Table 2, while one-way ANOVA with Tukey posthoc test multiple comparisons for kappa, highlighting the differences in cooperativity between the AMPs and antibiotics (B). ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.  (Figure 1) and present a comparison of the pharmacodynamic parameters obtained from challenges with AMPs, temporin L, a combination of temporin B and temporin L or, for comparison pleurocidin, 31 and existing clinically relevant antibiotics ( Table   2). The bacteria were not challenged with temporin B alone since this AMP lacks potency and the synergy screen data (Table 1) indicate that, where modest synergy in potency is observed, the activity is largely attributed to temporin L, which is never present at less than 1/2 its MIC. While a variety of antibiotics are used to treat Staphylococcus aureus infections, many strains are now multidrug resistant, only some antibiotics are bactericidal, and some may be restricted according to infection setting. EMRSA-15 is resistant to beta-lactams, second generation fluoroquinolones such as ciprofloxacin, and third generation cephalosporins such as ceftazidime. It is sensitive to aminoglycosides including tobramycin and Parameters are the average (3 s.f.) and standard error of values obtained from pharmacodynamic fits of three or more independently repeated experiments that generated log transformed CFU measurements. The standard error represents biological and technical variability between the independently repeated experiments (and not uncertainty). 60 . b Assay conducted in CA-MHB due to the requirement of daptomycin for Ca 2+ ions for activity. Values that differ significantly (one-way ANOVA with a Tukey posthoc test; p < 0.01) with respect to temporin L are shown in bold. Biochemistry pubs.acs.org/biochemistry Article gentamicin, glycopeptides such as telavancin and vancomycin and daptomycin. All these may be bactericidal, but vancomycin has been found to have only bacteriostatic activity against some MRSA 32,33 while use of daptomycin is more limited, e.g., since its inhibition by pulmonary surfactant ensured it failed to meet noninferiority criteria in clinical trials for communityacquired pneumonia.
Here linezolid, as expected, and vancomycin are found to be bacteriostatic against EMRSA-15 and are not considered further. The peptides and the clinically relevant daptomycin and aminoglycoside antibiotics tobramycin and gentamicin are bactericidal. However, pleurocidin, the combination of temporin L and temporin B, and temporin L alone all kill EMRSA-15 at a much faster rate than either aminoglycoside or daptomycin (p < 0.0001), as evidenced by ψ min , the minimum growth rate ( Table 2). The cooperativity of the dose dependent activity, as characterized by the steepness of the slope in a dose−response curve and the parameter kappa, reveals a potential benefit of challenging EMRSA-15 with a combination of temporin L and temporin B rather than temporin L alone. The cooperativity of the response to challenge with temporin L compares poorly with that to pleurocidin (p < 0.0001) (Table 2; Figure 1B). However, when used in combination with temporin B, kappa increases for the combination when compared with temporin L alone (p = 0.0315) ( Figure 1B, Table 2). Indeed, only the cooperativity of the response to challenge with the combination, but not temporin L alone, is greater than that achieved with either tobramycin (p = 0.0043) or gentamicin (p = 0.002).
The cooperativity of the dose dependent activity for both temporin L and the combination of temporin L/B is greater when the experiment is repeated in Luria−Bertani broth ( Figure S1). While the other parameters are similar in both media, in LB, kappa for the combination is nearly double that obtained in MHB. While in this media, the addition of temporin B to temporin L alone does not increase kappa (p = 0.9994), it does when buforin II is also present (p = 0.0023); LB is the only media in which we have found antibacterial activity with buforin II in broth microdilution assays, 34 and here we identified synergy between buforin II and both temporin L (FIC = 0.56) and the combination of temporin L and temporin B (FIC = 0.5) but not temporin B (FIC = 1) using checkerboard assays. In contrast, adding buforin II to temporin L alone does not lead to any increase in kappa (p = 0.2586) and may reduce it. The combination of all three peptides in LB produces a dose−response curve with a kappa value almost 6 times that for the corresponding experiment with temporin L alone in MHB indicating there is a substantial scope for the cooperativity of AMP bactericidal activity to vary according to the chemical environment.
Temporin L and Temporin B Form Hetero-Oligomers in Models of the Gram-Positive Plasma Membrane.
Since the in vitro pharmacodynamic study implies a possible interaction between temporin L and temporin B and since it is widely accepted that the main factor affecting the activity of AMPs is their interaction with the bacterial plasma cell membrane, we sought to identify whether either of these peptides modifies the membrane interaction of the other using   (Figures S2 and S3); binding and insertion ( Figure S4); peptide-lipid hydrogen bonding ( Figures S5 and S6); the formation of both homo-and heterooligomers in the bilayer ( Figure 2); and peptide induced lipid disordering (Figure 3). On binding to the POPG bilayer, temporin B does not adopt α-helix conformations, as the Ramachandran contour plots, representing phi and psi dihedral angles averaged over the duration of the simulation, indicates that the peptides adopt a type II β-turn conformation ( Figure S2A). In contrast, the majority of residues in temporin L (Trp4-Gly10) do adopt αhelix conformation. However, some residues may also adopt the type II β-turn ( Figure S2C). These residues are located at the N-and C-termini, and these regions also exhibit greater conformational flexibility, as measured by the circular variance of the psi dihedral angle ( Figure S3G). When temporin L is combined with temporin B, conformational flexibility in temporin L is reduced across the whole peptide ( Figure  S3C,D), in particular in the N-and C-termini ( Figure S3G,H), the α-helix conformation is extended (Gln3-Arg11) and evidence of type II β-turn diminishes ( Figure S2D). In contrast, temporin B experiences considerable conformational flexibility whether temporin L is present or not ( Figure  S3A,B,E,F) and there are no notable changes in conformation ( Figure S2B).
Since previous work has shown that temporin L acts to prevent oligomerization of either temporin A or temporin B in lipopolysaccharide (LPS), and we have separately shown that both temporin B and temporin L form oligomers when they insert into model bilayers, 14,15 we next assessed whether either peptide inhibited the membrane penetration ( Figure S3) and the interaction of the peptides with the bilayer (Figures S5 and  S6) and characterized any aggregates that formed ( Figure 2). As previously shown, both temporin B and temporin L penetrate the membrane via their N-termini and this is not substantially altered when the two different peptides are applied to the membrane in combination ( Figure S4).
The initial insertion is completed within 75 ns in all simulations, though penetration of temporin B is a little faster and deeper in the combination than when applied alone ( Figure S4A). Penetration of either temporin B or L is therefore not inhibited by the presence of the other temporin and, indeed, that of temporin B may be facilitated by temporin L. Consistent with this, neither the total number of peptidelipid hydrogen bonds ( Figure S5) nor the pattern of residue specific peptide-lipid hydrogen bonds ( Figure S6) is altered, for either temporin L or temporin B, when the membranes are challenged with the peptides in combination.
The effect of combining temporin B and temporin L is clearer following analysis of peptide−peptide oligomerization in the membrane ( Figure 2). As can be observed ( Figure 2B), and as was predominantly the case throughout the 200 ns duration of the simulation (Figure 2G), small heterooligomers, usually comprising two or three peptides, formed in POPG membranes. For temporin B, trimers (Figure 2A) and occasionally tetramers were frequently observed in analogous simulations, 14 and these are now shown to endure throughout the extended simulation ( Figure 2G), while temporin L forms trimers ( Figure 2C), tetramers, and in one simulation, a stable pentamer, 15 and these are retained as the simulation is extended to 200 ns ( Figure 2G). For the combination there are only four, as opposed to eight, of each temporin molecule in each simulation, and this may impact the probability of higher or lower order oligomerization and the ability to draw conclusions about the size distribution of resulting pores. However, the likely preference of each peptide for hetero-oligomerization over homo-oligomerization does provide support for a synergistic effect in the target membrane. This is revealed by analysis of the number of contacts between peptide monomers in each simulation ( Figure 2D−F). By chance, hetero-oligomeric contacts should predominate over homo-oligomeric contacts at a ratio of 4:3. Instead homooligomers of either temporin B or temporin L are very rare while hetero-oligomers are much more frequent (hetero-to homo-ratios 158:1 temporin B; 5.4:1 temporin L). With the exception of Arg11 in temporin L, the residues in each peptide involved in mediating assembly are hydrophobic, are located in the same positions in both temporin B and L, do not change substantially whether hetero-or homo-oligomers are being formed, and are not involved in hydrogen bonding with the lipid bilayer (Figure 2D−F; Figure S6).
While spontaneous pore formation in membranes is rarely observed in simulations when peptides start in the water phase, 35 the lipid disorder associated with their formation, in such studies, 36,37 is observed for some peptides irrespective of whether pores form or not. 14,38,39 The lipid disorder is greatest in those lipids associated with the peptide while order may increase for nonassociated lipids, 36,37 and in our previous work the same effect was observed for magainin 2, pleurocidin and its analogues, and temporin B. 14,31,38 Here, both temporin B ( Figure 3A) and temporin L ( Figure 3B) are observed to strongly disorder POPG lipids located within 4 Å of any peptide, with temporin L having a greater effect. In contrast, the disordering effect of temporin L on the whole bilayer is less noticeable compared with that of temporin B ( Figure 3C,D). When the peptides are applied in combination, the local disordering effect of both peptides is attenuated ( Figure 3A,B) while the impact on the whole bilayer is intermediate between that achieved with either peptide alone ( Figure 3C,D).
Temporin B Modulates Channel Activity Induced by Temporin L in Model Membranes. We made use of the port-a-patch automated patch-clamp system from Nanion Technologies (Munich, Germany) to determine whether the addition of temporin B modifies the ability of temporin L to disrupt DPhPG bilayers, mimicking Gram-positive bacteria cytoplasmic membranes (Figure 4). 23,24 Our experimental approach involves finding the lowest concentration of peptide that induces detectable conductance and then measuring the latency (the time taken for conductance to commence after addition of peptide) and recording whether the membrane is ultimately broken, and quantifying any characteristic channellike activity (well-defined events with discrete opening levels). Previously, we showed that temporin B does induce conductance in DPhPG bilayers but at a relatively high concentration of 35 μM. 14 It induces irregular conductance activity, and no evidence of regular channel formation was detected. Conductance activity does however appear relatively quickly after temporin B administration, and the membrane soon ruptures. In contrast, we have previously shown that Biochemistry pubs.acs.org/biochemistry Article temporin L does induce channel-like activity that endures, and this is achieved with less peptide (10 μM) than is required for temporin B. 15 Here, we find that combining temporin B and temporin L, in a 3.5:1 molar ratio reflecting their differing potency when used alone, substantially affects the ability to induce conductance. In combination, the concentrations of the peptides required to induce conductance are 12-fold lower than when each peptide is used alone. Channel-like activity is detected ( Figure 4A), and it appears more rapidly than when temporin L is applied alone ( Figure 4C). However, the amplitude, conductance, and estimated pore radii are much smaller than those observed for temporin L alone ( Figure  4A,B, Table 3). Patch-clamp therefore reveals the combination of temporin L and temporin B induces channel-like activity at much lower concentrations and faster, but the channels are much smaller than achieved with temporin L alone.

■ DISCUSSION
The discovery of the temporin peptides 11 in the skin secretion of Rana temporaria has precipitated a large body of work seeking to understand and develop those AMPs with the greatest antimicrobial activity into useful antibiotics. 9,10,39 Ten temporin peptides were initially described, and they share extensive sequence similarity, ranging from 76.9% to 100% relative to temporin B (Table 4). Though all were active against Bacillus megaterium, only temporins A, B, F, G, and L were active against Escherichia coli when tested individually, i.e., those carrying at least a +2 nominal charge and 13 residues in length. Subsequently, attention has been largely focused on temporins L, B, and A, 39−50 despite temporins F and G being produced at similar levels to temporins A and B, and temporin C being the most abundant of them all. 11 Temporin L has greater antibacterial potency against Gram-negative bacteria, binds lipopolysaccharide, and hence has antiendotoxin properties but is also relatively cytotoxic. 40−47 Temporins A and B are more active against Gram-positive bacteria though analogues of temporin B have been produced with a broader spectrum of activity. 49 Temporins A and B have also been shown to act in synergy with temporin L against Gram-negative bacteria and the mechanism for this synergy has been explored using biophysical methods. 12,50 However, since the other temporins have received less attention, it is unclear what the biological benefits are of producing such a set of closely related peptides nor what the relatively minor changes in amino acid sequence, at least between temporins A−K (Table 4), achieve. The previous studies of synergy between temporin L and either temporins A or B have focused on Gram-negative bacteria to understand gains in antibacterial potency. These reveal that temporin L affects the oligomerization of temporin B in lipopolysaccharide environments. 12,50 Here, though FIC data would indicate that there is no interaction between temporin L and temporin B save for possibly a weak synergistic effect in some strains (additive elsewhere), three separate pieces of evidence indicate there is an interaction between temporin L and temporin B that will also influence their combined activity against Gram-positive bacteria.
First, we show that the addition of temporin B to either temporin L in MHB or temporin L/buforin II in LB increases the cooperativity of the dose dependent rate of bacterial killing, and that this is greater than that observed for antibiotics. A limitation of our study is the absence of in vitro PD data for temporin B alone, and we cannot conclude whether the combination of temporin L and temporin B has greater cooperativity than both individual components or whether temporin B has higher cooperativity than temporin L and the combination then matches this. Instead, since the MIC of temporin L in combination with temporin B is never less than half of its MIC when applied alone, we have demonstrated that the effect of the combination is to retain the potency of The average time taken for conductance to begin after peptide addition (latency) shows conductance begins more rapidly for the combination than temporin L alone (C). One-way ANOVA with a Tukey posthoc test, *p < 0.05, ***p < 0.001. Biochemistry pubs.acs.org/biochemistry Article temporin L but with enhanced cooperativity derived from the addition of temporin B, well below its own MIC. The effects and mechanisms of adding buforin II, as a component of antimicrobial peptide combinations, warrant further investigation, not least because the effect of its addition in the present study is not clear-cut. The present observations are reported here for two reasons. First, buforin II is a 21 amino acid histone H2A fragment, initially identified in the Asian toad Bufo bufo garagrizans, 51 but its sequence is also found in the Rana temporaria (and mammalian) histone H2A, and hence there is potential for it interacting with a wide variety of AMPs in different organisms. Second, buforin II accumulates within bacteria, has high affinity for nucleic acids, and its antibacterial mechanism of action is independent of membrane lysis and hence completely different to that of either temporin L or temporin B. 51−53 Therefore, the increases in cooperativity, obtained by combining temporin B with temporin L alone (in MHB) or with buforin II (in LB), are two examples of AMPs with differing mechanisms from the same organism combining to produce bactericidal activity with greater cooperativity. This is consistent with previous work that has shown diverse AMPs, but from different organisms, display greater cooperativity than antibiotics when killing Escherichia coli MG1655 and that this is enhanced when these AMPs are used in three-way combinations. 7,54 The present data indicate that the possible impact of bacterial growth conditions, and other factors, on AMP pharmacodynamics should be explored in more depth but are sufficient to conclude that combining AMPs has potential in further distinguishing their in vitro pharmacodynamic properties from those of bactericidal antibiotics. By extension, future work may now test the theory that the more cooperative pharmacodynamic profile achieved with combinations of AMPs mitigates the risk of resistance developing and hence a rationale for the evolution of synergistic AMP families within individual species. DPhPG membranes were challenged with 10 μM temporin L alone or a combination of 0.84 μM temporin L and 2.92 μM temporin B. Temporin B alone does not induce channel-like activity. Level 1 is present in 5/6 traces acquired, Level 2 is present in 3/6 out of 6 traces, and Level 3 is present in 2/6 traces. Each parameter represents a range of events around each defined level, detected in between 2 and 5 traces, and the standard error reflects the variability in such events between traces. b Data previously reported. 15 Table 4. Alignment of Temporin Peptides Sequences and Their Physicochemical Characteristics a a Average hydrophobicity is given on the whole-residue hydrophobicity octanol-interface scale (ΔG woct − ΔG wif ) based on the free energy of transfer from water to palmitoyloleoylphosphatidylcholine and to n-octanol. 61 All peptides are considered amidated at the C-terminus, but this is not considered in the hydrophobicity calculation. In temporins A−G, Pro3, Gly6, Leu9, and Leu13 are absolutely conserved. Leu9 and Leu13 are also conserved in temporin L. Biochemistry pubs.acs.org/biochemistry Article Second, we show that temporin B and temporin L readily form hetero-oligomers in MD simulations of challenge of a model of the Gram-positive plasma membrane. Although there are many ways in which two different AMPs may influence the activity of each other, the formation of hetero-oligomers has been observed for other AMPs that are known to act in synergy; magainin 2 and PGLa, which are structurally related and from the same organism (Xenopus laevis), is a very wellstudied example. 55−59 In coarse-grain MD simulations, magainin 2 was shown to fix the membrane inserting state of PGLa, which otherwise continuously inserts and leaves the membrane, and aid recruitment of other peptides into heterodimers involved in the formation of transmembrane pores, 59 explaining the observed increase in membrane affinity of the mixture. 58 Here we use atomistic simulations to sample a shorter time scale, but while the membrane insertion of either temporin L or temporin B is largely unaffected by the presence of the other, the observation of hetero-oligomer formation, and concomitant restriction of temporin L homo-oligomer formation and lipid acyl-chain disordering can be expected to be manifested in altered disruptive effects of the peptides on the target plasma membrane.
Third, we use conductance measurements to show that the interaction between the temporin L/temporin B combination and model membranes fundamentally differs to that observed when either peptide is applied alone with conductance events observed more quickly and with much lower amounts of each peptide when applied in combination than when applied alone. The conductance manifests as regular channel-like events, similar to those produced with temporin L alone but of a much lower conductance and calculated size. To achieve greater cooperativity, the combination should suppress bactericidal activity at lower AMP concentrations but enhance it at higher concentrations. It is possible that the ability to induce conductance in model membranes with much less peptide, and faster, is a manifestation of enhanced bactericidal membrane activity of the temporin B/temporin L combination. However, unless other factors intervene to substantially reduce antibacterial activity overall, we would also expect to see a considerable increase in antibacterial potency for the combination. However, this is inconsistent with the modest synergy observed, as described by the FIC. Alternatively, the low conductance events observed for the combination may be insufficient for a bactericidal effect and this then would be consistent with temporin B preferentially forming heterooligomers with temporin L that are less effective than temporin L homo-oligomers. Only at higher relative concentrations of temporin L do high conductance channels form and hence cooperativity is enhanced. Therefore, the present biophysical data establishes high probability of an interaction between temporin L and temporin B in the target plasma membrane and provides clues as to how the greater cooperativity in bactericidal activity is achieved, a complete mechanistic understanding will require a future investigation of dosedependent effects in both patch-clamp studies and MD simulations.

■ CONCLUSION
Combining two or, potentially, more antimicrobial peptides from the same organism improves the in vitro pharmacodynamic properties of the bactericidal action. For temporin L and temporin B, this is likely achieved through modification of aggregates formed by the peptides in the target membrane.
The resulting ability of temporin L to induce channel-like conductance suggests an evolutionary benefit for generating a family of AMPs and a more important role for those AMPs that alone have low antibacterial potency.
Further in vitro pharmacodynamic data and analysis of the MD simulations (Figures S1−S6) (PDF)