Synthetic Defined (SD) Medium

2% glucose

6.7 g/L yeast nitrogen base without amino acids

Amino acids:

• 20 mg/L histidine

• 120 mg/L leucine

• 60 mg/L lysine

• 20 mg/L arginine

• 20 mg/L tryptophan

• 20 mg/L tyrosine

• 40 mg/L threonine

• 20 mg/L methionine

• 50 mg/L phenylalanine

20 mg/L uracil

20 mg/L adenine

20 g/L agar (omit for liquid medium)

Include additional amino acids that may be required for the growth of an auxotrophic strain. Prepare in H₂O and sterilize by autoclaving. For agar plates, fill sterile Petri dishes with ∼25 mL of autoclaved medium.

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