Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis
- Kevin P. Brady1,
- Lucy B. Rowe2,
- Helen Her1,
- Tamara J. Stevens3,
- Janan Eppig2,
- Daniel J. Sussman4,
- James Sikela3, and
- David R. Beier1,5
- 1Division of Genetics, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115; 2The Jackson Laboratory, Bar Harbor, Maine 04605; 3Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262; 4Division of Human Genetics, University of Maryland School of Medicine, Baltimore, Maryland 21201
Abstract
We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3′-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.
Footnotes
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↵5 Corresponding author.
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E-MAIL beier{at}rascal.med.harvard.edu; FAX (617) 232-4623.
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- Received July 23, 1997.
- Accepted October 6, 1997.
- Cold Spring Harbor Laboratory Press
