Site-specific Genetic Recombination Promoted by the FLP Protein of the Yeast 2-micron Plasmid In Vitro
This extract was created in the absence of an abstract.
Excerpt
The yeast 2-micron plasmid is a 6318-bp circular DNA molecule present at 50–100 copies per cell in most yeast strains (Broach 1981). The plasmid has been sequenced (Hartley and Donelson 1980) and has been utilized as a cloning vector in yeast (Botstein and Davis 1982). A notable feature of the sequence of this plasmid is a 599-bp inverted repeat, which divides the plasmid into two roughly equivalent segments. Recombination between these repeats inverts one segment of the plasmid relative to the other (Broach et al. 1982). This results in two forms of the plasmid, A and B, which occur in equal concentrations in plasmid DNA isolated from yeast cells. In addition, 10–20% of the 2-micron plasmid DNA isolated from yeast is present in dimeric or other multimeric forms (Royer and Hollenberg 1977). These multimers could arise in vivo due to intramolecular recombination within a θ-structure replication intermediate or could be...
