Yeast Chromosomal DNA: Size, Structure, and Replication
This extract was created in the absence of an abstract.
Excerpt
The yeast Saccharomyces cerevisiae is a eukaryotic organism with a very small DNA content. Chemical analysis indicates that there are between 0.84 and 1.2 × 1010 daltons of DNA in a haploid nucleus (Hartwell, 1970a). DNA renaturation experiments have shown that there is little redundant DNA (Britten and Kohne, 1968) and that most of the DNA has a kinetic complexity of 0.92 × 1010 daltons (Bicknell and Douglas, 1970). More striking perhaps is the DNA content of an average yeast chromosome. Since genetic analysis has shown that there are at least 17 chromosomes (Mortimer and Hawthorne, 1973), the average DNA content is no more than 5.4 × 108 daltons. The size of these chromosomes, as judged by recombination frequencies (Mortimer and Hawthorne, 1973), ranges from less than 50 map units to greater than 250 map units. In addition, the number of mapped genes ranges from 3 per chromosome to 18...
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↵* Present Address: Division of Microbiology, National Institutes for Medical Research, Mill Hill, London, N.W.7, England.
